IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ serra-2006-2 RORalpha-mediated Purkinje cell development determines disease severity in adult SCA1 mice. mouse-brain-cerebellum Mus musculus brain cerebellum brain_cerebellum [Cerebellar] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Serra HG., Duvick L., Zu T., Carlson K., Stevens S., Jorgensen N., Lysholm A., Burright E., Zoghbi HY., Clark HB., Andresen JM., Orr HT. A parasagittal cerebellar section from a 12 week old SCA1[30Q] heterozygous mouse was simultaneously stained with antibodies against ATXN1 and RORalpha. orrxx002@umn.edu Cell (0092-8674) 2006 Only protein-protein interactions 10 imex curation serra-2006-4 RORalpha-mediated Purkinje cell development determines disease severity in adult SCA1 mice. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Serra HG., Duvick L., Zu T., Carlson K., Stevens S., Jorgensen N., Lysholm A., Burright E., Zoghbi HY., Clark HB., Andresen JM., Orr HT. A parasagittal cerebellar section from a 12 week old SCA1[30Q] heterozygous mouse was simultaneously stained with antibodies against ATXN1 and RORalpha. orrxx002@umn.edu Cell (0092-8674) 2006 Only protein-protein interactions 10 imex curation serra-2006-1 RORalpha-mediated Purkinje cell development determines disease severity in adult SCA1 mice. mouse-purkinje cell Mus musculus brain-purkinje_cell Purkinje cell Purkinje cells can be found either as 1] Purkinje fibers in the inner ventricular walls of the heart, that conduct an electrical stimulus that enables the heart to contract in a coordinated fashion - or 2]Purkinje neurons, a class of GABAergic neurons located in the cerebellar cortex. confocal microscopy confocal microscopy immunostaining immunostaining Serra HG., Duvick L., Zu T., Carlson K., Stevens S., Jorgensen N., Lysholm A., Burright E., Zoghbi HY., Clark HB., Andresen JM., Orr HT. A parasagittal cerebellar section from a 12 week old SCA1[30Q] heterozygous mouse was simultaneously stained with antibodies against ATXN1 and RORalpha. orrxx002@umn.edu Cell (0092-8674) 2006 Only protein-protein interactions 10 imex curation atx1_mouse Ataxin-1 Spinocerebellar ataxia type 1 protein homolog Atxn1 Sca1 protein protein mouse Mus musculus Mouse MKSNQERSNECLPPKKREIPATSRPSEEKATALPSDNHCVEGVAWLPSTPGIRGHGGGRHGSAGTSGEHGLQGMGLHKALSAGLDYSPPSAPRSVPTANTLPTVYPPPQSGTPVSPVQYAHLSHTFQFIGSSQYSGPYAGFIPSQLISPSGNPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPPPQQHLSRAAGLVNPGSPPPPTQQNQYIHISSSPQSSGRATSPPPIPVHLHPHQTMIPHTLTLGPSSQVVVQYSDAGGHFVPRESTKKAESSRLQQAMQAKEVLNGEMEKSRRYGASSSVELSLGKASSKSVPHPYESRHVVVHPSPADYSSRDTSGVRGSVMVLPNSSTPSADLEAQQTTHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGGLPQHLVIPGNQPLLIPVGSPDMDTPGAASAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPTTASPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEESHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKQAKTDSLAGSRHRYAEQENGINQGSAQVLSENGELKFPEKIGLPAAPFLSKIEPSKPTATRKRRWSAPETRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK rora_mouse Nuclear receptor ROR-alpha Retinoid-related orphan receptor-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 Rora Nr1f1 Rzra RAR-related orphan receptor A protein protein mouse Mus musculus Mouse MESAPAAPDPAASEPGSSGSEAAAGSRETPLTQDTGRKSEAPGAGRRQSYASSSRGISVTKKTHTSQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSTYMDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG kat5_mouse Histone acetyltransferase KAT5 Kat5 Htatip Tip60 Lysine acetyltransferase 5 Histone acetyltransferase HTATIP 60 kDa Tat-interactive protein protein protein mouse Mus musculus Mouse MAEVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGSARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW atx1_human Ataxin-1 ATXN1 ATX1 SCA1 Spinocerebellar ataxia type 1 protein protein protein human Homo sapiens Human MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK kat5_human Histone acetyltransferase KAT5 KAT5 TIP60 HTATIP Lysine acetyltransferase 5 cPLA(2)-interacting protein Histone acetyltransferase HTATIP 60 kDa Tat-interactive protein protein protein human Homo sapiens Human MAEVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW rora-atxn1 ATXN1 coimmunoprecipitated with ROR-alpha 570272 N/A 570275 unspecified role unspecified role prey prey N/A 570276 unspecified role unspecified role bait bait physical association physical association false false false 5 A This interaction did not take place in the Sca1-/- mice. atxn1-rora-2 ROR-alpha coimmunoprecipitated with ATXN1 570272 N/A 570276 unspecified role unspecified role prey prey N/A 570275 unspecified role unspecified role bait bait physical association physical association false false false 5 A This interaction did not take place in the Sca1-/- mice. htatip-atxn1-1 ATXN1 coimmunoprecipitated with Tip60 570272 N/A 570277 unspecified role unspecified role bait bait N/A 570275 unspecified role unspecified role prey prey physical association physical association false false false 5 C atxn1-htatip Tip60 coimmunoprecipitated with ATXN1 570272 N/A 570275 unspecified role unspecified role bait bait N/A 570277 unspecified role unspecified role prey prey physical association physical association false false false 5 C atxn1-htatip-2 GST-ATXN1 pulls down Tip60 570293 N/A 570278 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) N/A 570277 unspecified role unspecified role prey prey mouse-brain-cerebellum Mus musculus brain cerebellum brain_cerebellum [Cerebellar] physical association physical association false false false 5 D The amount of Tip60 pulled down increased as the length of the polyglutamine tract increased from 30 to 53 to 82. atxn1-htatip-3 GST-ATXN1pulls down in vitro translated Tip60 570293 N/A 570279 unspecified role unspecified role prey prey in vitro In vitro N/A 570278 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 5 F The increasing of the polyglutamine tract length in ATXN1 did not affect the amount of Tip60 pulled down by ATXN1. htatip-atxn1 ATXN1 coimmunoprecipitated with Tip60 570273 N/A 570279 unspecified role unspecified role bait bait N/A 570278 unspecified role unspecified role prey prey physical association physical association false false false 5 E More ATXN1-82Q was coimmunoprecipitated with Tip60 than was ATXN1-30Q, further suggesting an effect of the polyglutamine sequence on the interaction of ATXN1 with Tip60. atxn1-htatip-1 ATXN1 coimmunoprecipitated with Tip60 570273 N/A 570278 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 570279 unspecified role unspecified role prey prey physical association physical association false false false 6 Of the ATXN1 fragments the C-terminal fragment (568-816) and the N-terminal fragment (1-360) interacted to a lesser extent with Tip60 than the two fragments containing aa 425-547 (fragments 250-547 and 425-689). The N-terminal fragment (1-360) of ATXN1 with the wild-type 30Q polyglutamine stretch did not interact strongly with Tip60 but the same fragment with the 82Q mutant polyglutamine stretch did. The two fragments of ATXN1 containing amino acids 425-547, fragment III and IV, interacted with Tip60 at least as well as intact ATXN1[30Q] did. In contrast, fragments lacking this portion of ATXN1 interacted to a lesser extent with Tip60 than did full-length ATXN1[30Q]. Notably, these include the N-terminal 360 amino-acid fragment containing the polyglutamine stretch and the C-terminal fragment spanning amino acids 568-816 containing the AXH domain AXH domain atxn1-rora Confocal microscopy reveals that ATXN1 and ROR-alpha colocalize 570274 N/A 570275 unspecified role unspecified role neutral component neutral component N/A 570276 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S2