IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nakajima-2006-1 Identification and characterization of Arabidopsis gibberellin receptors. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakajima M., Shimada A., Takashi Y., Kim YC., Park SH., Ueguchi-Tanaka M., Suzuki H., Katoh E., Iuchi S., Kobayashi M., Maeda T., Matsuoka M., Yamaguchi I. All yeast two hybrid assays were in the presence of 10x-5 M Giberrellin. nkjm@pgr1.ch.a.u-tokyo.ac.jp Plant J. (0960-7412) 2006 Only protein-protein interactions imex curation gai_arath DELLA protein GAI GAI RGA2 At1g14920 F10B6.34 Gibberellic acid-insensitive mutant protein Restoration of growth on ammonia protein 2 GRAS family protein 3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRDHHHHHHQDKKTMMMNEEDDGNGMDELLAVLGYKVRSSEMADVAQKLEQLEVMMSNVQEDDLSQLATETVHYNPAELYTWLDSMLTDLNPPSSNAEYDLKAIPGDAILNQFAIDSASSSNQGGGGDTYTTNKRLKCSNGVVETTTATAESTRHVVLVDSQENGVRLVHALLACAEAVQKENLTVAEALVKQIGFLAVSQIGAMRKVATYFAEALARRIYRLSPSQSPIDHSLSDTLQMHFYETCPYLKFAHFTANQAILEAFQGKKRVHVIDFSMSQGLQWPALMQALALRPGGPPVFRLTGIGPPAPDNFDYLHEVGCKLAHLAEAIHVEFEYRGFVANTLADLDASMLELRPSEIESVAVNSVFELHKLLGRPGAIDKVLGVVNQIKPEIFTVVEQESNHNSPIFLDRFTESLHYYSTLFDSLEGVPSGQDKVMSEVYLGKQICNVVACDGPDRVERHETLSQWRNRFGSAGFAAAHIGSNAFKQASMLLALFNGGEGYRVEESDGCLMLGWHTRPLIATSAWKLSTN gid1a_arath Gibberellin receptor GID1A GID1A At3g05120 T12H1.8 GID1-like protein 1 CXE10 GID1L1 AtCXE10 Carboxylesterase 10 Protein GA INSENSITIVE DWARF 1A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGESGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC rga_arath DELLA protein RGA RGA GRS RGA1 At2g01570 F2I9.19 Repressor on the ga1-3 mutant GAI-related sequence Restoration of growth on ammonia protein 1 GRAS family protein 10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRDHHQFQGRLSNHGTSSSSSSISKDKMMMVKKEEDGGGNMDDELLAVLGYKVRSSEMAEVALKLEQLETMMSNVQEDGLSHLATDTVHYNPSELYSWLDNMLSELNPPPLPASSNGLDPVLPSPEICGFPASDYDLKVIPGNAIYQFPAIDSSSSSNNQNKRLKSCSSPDSMVTSTSTGTQIGGVIGTTVTTTTTTTTAAGESTRSVILVDSQENGVRLVHALMACAEAIQQNNLTLAEALVKQIGCLAVSQAGAMRKVATYFAEALARRIYRLSPPQNQIDHCLSDTLQMHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGLQWPALMQALALREGGPPTFRLTGIGPPAPDNSDHLHEVGCKLAQLAEAIHVEFEYRGFVANSLADLDASMLELRPSDTEAVAVNSVFELHKLLGRPGGIEKVLGVVKQIKPVIFTVVEQESNHNGPVFLDRFTESLHYYSTLFDSLEGVPNSQDKVMSEVYLGKQICNLVACEGPDRVERHETLSQWGNRFGSSGLAPAHLGSNAFKQASMLLSVFNSGQGYRVEESNGCLMLGWHTRPLITTSAWKLSTAAY rgl1_arath DELLA protein RGL1 RGL1 RGAL At1g66350 T27F4.10 RGA-like protein 1 GRAS family protein 9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKREHNHRESSAGEGGSSSMTTVIKEEAAGVDELLVVLGYKVRSSDMADVAHKLEQLEMVLGDGISNLSDETVHYNPSDLSGWVESMLSDLDPTRIQEKPDSEYDLRAIPGSAVYPRDEHVTRRSKRTRIESELSSTRSVVVLDSQETGVRLVHALLACAEAVQQNNLKLADALVKHVGLLASSQAGAMRKVATYFAEGLARRIYRIYPRDDVALSSFSDTLQIHFYESCPYLKFAHFTANQAILEVFATAEKVHVIDLGLNHGLQWPALIQALALRPNGPPDFRLTGIGYSLTDIQEVGWKLGQLASTIGVNFEFKSIALNNLSDLKPEMLDIRPGLESVAVNSVFELHRLLAHPGSIDKFLSTIKSIRPDIMTVVEQEANHNGTVFLDRFTESLHYYSSLFDSLEGPPSQDRVMSELFLGRQILNLVACEGEDRVERHETLNQWRNRFGLGGFKPVSIGSNAYKQASMLLALYAGADGYNVEENEGCLLLGWQTRPLIATSAWRINRVE rgl2_arath DELLA protein RGL2 RGL2 SCL19 At3g03450 T21P5.13 RGA-like protein 2 Scarecrow-like protein 19 GRAS family protein 15 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRGYGETWDPPPKPLPASRSGEGPSMADKKKADDDNNNSNMDDELLAVLGYKVRSSEMAEVAQKLEQLEMVLSNDDVGSTVLNDSVHYNPSDLSNWVESMLSELNNPASSDLDTTRSCVDRSEYDLRAIPGLSAFPKEEEVFDEEASSKRIRLGSWCESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGAMGKVATYFAQALARRIYRDYTAETDVCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPSFRLTGIGPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESETLVVNSVFELHRLLARSGSIEKLLNTVKAIKPSIVTVVEQEANHNGIVFLDRFNEALHYYSSLFDSLEDSYSLPSQDRVMSEVYLGRQILNVVAAEGSDRVERHETAAQWRIRMKSAGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMIGWQTRPLITTSAWKLA gid2_arath F-box protein GID2 Protein SLEEPY 1 GID2 SLY1 T22A6.40 At4g24210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRSTTDSDLAGDAHNETNKKMKSTEEEEIGFSNLDENLVYEVLKHVDAKTLAMSSCVSKIWHKTAQDERLWELICTRHWTNIGCGQNQLRSVVLALGGFRRLHSLYLWPLSKPNPRARFGKDELKLTLSLLSIRYYEKMSFTKRPLPESK gid1b_arath Gibberellin receptor GID1B GID1B At3g63010 T20O10.110 GID1-like protein 2 CXE14 GID1L2 AtCXE14 Carboxylesterase 14 Protein GA INSENSITIVE DWARF 1B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGGNEVNLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHVDSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATNEGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSIEDSQSKSSPVLLTP gid1c_arath Gibberellin receptor GID1C GID1C At5g27320 F21A20.30 GID1-like protein 3 CXE19 GID1L3 AtCXE19 Carboxylesterase 19 Protein GA INSENSITIVE DWARF 1C protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVESRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFVNAECQ gid1-gia Directed Yeast 2 hybrid analysis reveals interaction between the GID1 protein and GIA protein 493659 N/A 493660 unspecified role unspecified role prey prey N/A 493661 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A. gid1-rga Directed Yeast 2 hybrid analysis reveals interaction between the GID1 protein and RGA protein 493659 N/A 493662 unspecified role unspecified role prey prey N/A 493661 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A and B. The interaction of the proteins could only be detected when the concetration of gibberellin was 10x-7 M. gid1-rgl1 Directed Yeast 2 hybrid analysis reveals interaction between the GID1 protein and RGL1 protein 493659 N/A 493663 unspecified role unspecified role prey prey N/A 493661 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid1-rgl2 Directed Yeast 2 hybrid analysis reveals interaction between the GID1 protein and RGL2 protein 493659 N/A 493664 unspecified role unspecified role prey prey N/A 493661 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid1-rgl3 Directed Yeast 2 hybrid analysis reveals interaction between the GID1 protein and RGL3 protein 493659 N/A 493661 unspecified role unspecified role bait bait N/A 493665 unspecified role unspecified role prey prey physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid2-rgl3-1 Directed Yeast 2 hybrid analysis reveals interaction between the GID2 protein and RGL3 protein 493659 N/A 493665 unspecified role unspecified role prey prey N/A 493666 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid2-gia Directed Yeast 2 hybrid analysis reveals interaction between the GID2 protein and GIA protein 493659 N/A 493666 unspecified role unspecified role bait bait N/A 493660 unspecified role unspecified role prey prey physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A. gid2-rga-1 Directed Yeast 2 hybrid analysis reveals interaction between the GID2 protein and RGA protein 493659 N/A 493662 unspecified role unspecified role prey prey N/A 493666 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A and B. The interaction of the proteins could only be detected when the concetration of gibberellin was 10x-8 M. gid2-rgl1 Directed Yeast 2 hybrid analysis reveals interaction between the GID2 protein and RGL1 protein 493659 N/A 493663 unspecified role unspecified role prey prey N/A 493666 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid2-rgl2 Directed Yeast 2 hybrid analysis reveals interaction between the GID2 protein and RGL2 protein 493659 N/A 493664 unspecified role unspecified role prey prey N/A 493666 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid3-rgl3-1 Directed Yeast 2 hybrid analysis reveals interaction between the GID3 protein and RGL3 protein 493659 N/A 493666 unspecified role unspecified role bait bait N/A 493665 unspecified role unspecified role prey prey physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid3-rga Directed Yeast 2 hybrid analysis reveals interaction between the GID3 protein and RGA protein 493659 N/A 493662 unspecified role unspecified role prey prey N/A 493667 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A and B. The interaction of the proteins could only be detected when the concetration of gibberellin was 10x-7 M. gid3-gia Directed Yeast 2 hybrid analysis reveals interaction between the GID3 protein and GIA protein 493659 N/A 493660 unspecified role unspecified role prey prey N/A 493667 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A. gid3-rgl1 Directed Yeast 2 hybrid analysis reveals interaction between the GID3 protein and RGL1 protein 493659 N/A 493663 unspecified role unspecified role prey prey N/A 493667 unspecified role unspecified role bait bait physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A . gid3-rgl2-1 Directed Yeast 2 hybrid analysis reveals interaction between the GID3 protein and RGL2 protein 493659 N/A 493667 unspecified role unspecified role bait bait N/A 493664 unspecified role unspecified role prey prey physical association physical association false false false Giberrellin (Ga) stimulates interaction. Figure 3 A .