IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ olson-2006-1 Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. mouse-ghft_1 Mus musculus immortalised somatotropic progenitor cells - SV40 transformed. ghft_1 mouse immortalized somatotropic progenitor cell Somatotropic progenitor cell in which GHF-1 transcription is first activated were immortalized by using the GHF-1 regulatory region to target SV40 T-antigen (Tag) tumorigenesis in transgenic mice anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu Cell (0092-8674) 2006 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 imex curation olson-2006-2 Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. Immobilized GST-fusion proteins were mixed with 293T cell lysates containing overexpressed, tagged beta-catenin protein. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu Cell (0092-8674) 2006 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 imex curation prop1_mouse Homeobox protein prophet of Pit-1 Prop1 Prop-1 Pituitary-specific homeodomain factor protein protein mouse Mus musculus Mouse MEAQRRSHQEKQTKGHACGRSLPEPRVASGTLISTVDRSSEAYRRLSGTELGRPKLCPQRGRPHSRRRHRTTFNPAQLEQLESAFGRNQYPDIWAREGLAQDTGLSEARIQVWFQNRRAKQRKQERSLLQPIAHLSTATFSGFLPESSAYPYTYGTPPPPAPCFPHPYSHSLPSQPSTAASLALPPQPEDWYPTLHPAPTGHLPCPPPPPMFPLSLETPKSWN ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL prop1-catenine 372275 N/A 372277 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 372278 unspecified role unspecified role prey prey physical association physical association false false false 4D prop1-catenine-1 372276 N/A 372277 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false homeodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 372278 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false lef1 region Arm repeats 5-9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4C The authors have described Prop1 protein with 225 AA while the swissprot entry points to an 223 AA sequence. An TrEMBL enty Q8CJ18 corresponding to 224 AA does exist. The sequences are most distinct in their C-terminal end which is the interacting region. Homeodomain of Prop1 shows weaker interaction than C-terminal. Q02248 overexpressed