MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint nagano-2006-1 PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation nagano-2006-2 PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation ml12b_mouse Myosin regulatory light chain 12B Mrlc2 Myosin regulatory light chain 2-B, smooth muscle isoform Myl12b Mylc2b Myosin regulatory light chain MRLC2 Myosin regulatory light chain 20 kDa protein protein mouse Mus musculus Mouse MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG mylip_mouse E3 ubiquitin-protein ligase MYLIP Mylip Inducible degrader of the LDL-receptor Myosin regulatory light chain-interacting protein RING-type E3 ubiquitin transferase MYLIP protein protein mouse Mus musculus Mouse MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKESLLAGHLQCSPEQAVELSALLAQTKFGDYNQNTAQYSYEDLCEKELSSSTLNSIVAKHKELEGISQASAEYQVLQIVSAMENYGIEWHAVRDSEGQKLLIGVGPEGISICKEDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRSDQSPPSSPLKSSDSSMSCSSCEGLSCQQTRVLQEKLRKLKEAMLCMACCEEEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI mint E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Stable or transient overexpression of MIR induces proteasomal degradation of MRLC inhibiting neurite outgrowth in presence of NGF. MIR counteracts the stabilization of MRLC by MIR-interacting saposin-like protein (MSAP/TMEM4) and reduces MSAP-stimulated neurite outgrowth myl12b-rps27a 372051 ml12b_mouse 372053 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 ubiq_mouse 372054 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association false false false F3B F3C mint mylip-myl12b 372052 mylip_mouse 372055 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 ml12b_mouse 372053 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association false false false F4C homomint mint