IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ishibashi-2006-1 A higher plant has three different types of RPA heterotrimeric complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 ishibashi-2006-2 A higher plant has three different types of RPA heterotrimeric complex. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 ishibashi-2006-3 A higher plant has three different types of RPA heterotrimeric complex. orysa Oryza sativa (Rice) Rice anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 rfa3_orysj Replication protein A 14 kDa subunit RPA3 RPA14 Replication factor A protein 3 Replication protein A 3 OsJ_01130 P0705D01.4 LOC_Os01g14980 Os01g0253600 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MDTSGPAAFVNGEILKMFVGRRVRTVVQAQREEGGLLIGQSTDGHQLTIKGASGAPMSHYVEIIGIAEPNQAIRAEVCTDFGENFDPAPFNGLCKLANGQMKDLFL rfa2a_orysj Replication protein A 32 kDa subunit A RPA2A OsRPA32-1 RPA32A Replication factor A protein 2A Replication protein A 2A OJ1124_D06.4 OsJ_08977 LOC_Os02g58220 Os02g0829100 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MMSFSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQISEAQQSGITGEKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTKSQIGSPAKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTEVLNVFREPANVESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSAFNE rfa2b_orysj Replication protein A 32 kDa subunit B RPA2B OsRPA32-2 RPA32B Replication factor A protein 2B Replication protein A 2B OJ1643_A10.19 LOC_Os02g42230 Os02g0633400 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MYGVGVGGGGGGNYDGGGGNASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDASQTNDDKSNFAVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGDYVIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQVNANTATGTPNQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMNDDHGVGVDYVSRRLNLPEETVGKIIIDQVDLGHLYATIDDHHYKSTMNG rfa2c_orysj Replication protein A 32 kDa subunit C RPA2C OsRPA32-3 RPA32C Replication factor A protein 2C Replication protein A 2C P0550B04.15 LOC_Os06g47830 Os06g0693300 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MAAAASYFSGTALMPSQRSGAPAPEYSAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALAADGGGDPVFSIDGVETTNVRVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRDTSYIQEGVYVKVQVNLMGFQAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQRQLPPSVQTNEYTHVPSSGGVRDYQVHFTPQVNQGLPPAVQTNTSTYVPLLGGVRDHQAHFAQVNQGQFSPAVQANTSTHLPFSGGVGEHQIHFTPKVNQGQFPPSVQTNTSAHVPYSGGFREHQVHFTPPVNQGQFPPAVQTNLYNHAASSGGVREQVHLTQANQFSAYSSTGGLQHDPQRMVLEALQQPDILALEHGAHVDELVRRTGMPKANIMGVVKHLAAAGFVYWTIDDNHVKSMCNG rfa1c_orysj Replication protein A 70 kDa DNA-binding subunit C RPA1C RPA70C Replication factor A protein 1C Replication protein A 1C OsJ_16860 P0016H04.11 LOC_Os05g02040 Os05g0111000 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MEPQLTPGAVQAIAEHPDGTGTIQPVLQVVDVRPVTTKNAPPTPKPAERFRMMLSDGVNTQQSMLATALNPLVKDATLRPGTVVQLTDFMCNTIQGKRIIIVVKLDVLQNDCIVIGNPKHYEPKSLTKEQDPNLQASVAQTNNGTYSGGASMLGPSVAPRAEQAASNSSYGGPYNSAQGMLGSSIGRTVEPGPANVSAVGSYGAISAQNTTNANMMQPTSQLNIMNANTMQPTSQLNTMNANTMQPTSQLSSLNPNQNQRFAAPASGGVFGPPGNAYGQPSRPSYQQPPPVYMNRGPASRNDSATRIIPITALNPYQPKWTIKARVTAKSDIRHWSNARSSGTVFSFDLLDAQGGEIRAQCWKESADKFFGQIEVGRVYLISRGSLKPAQKKYNTLNHDYEITLDIGLSTVEVCSDDDNSIPRLQYNFRQISELENMANETIVDLLGVVTSVSPSATIMRKIGTETRKRSIQLKDLSGRSIEVTLWGNFCDAEGQQLQLQCDSGSNPIIAFKGARVGDFNGKSVSTIGSTQLIINPDFPEVERLRQWYMTEGKTAPCISLSREMLNMGRTDARKTIAQIKDENLGRLEKPDWITVKAAISHVTTESFCYPACPKLLPVGRQCNKKAINNGDGMWHCDRCDESFQNPEYRYMLRFQIQDHTGSTYASAFDEAGEQIFGRKAGELFSIRNVDQDDAQFAEIIEGVRWHLYLFKLKVKEETYNDEQSLKCTAVKVEKLDPSKESNVLLGAIDNLLLDPKGQSDLAPNAGFTDPVGGHGAPTSSNAYAMNTGGVNQFGQQASISAGMSTPLAATRNLQTCSICGANGHSAQICHVGADMDMQETSAGGSSMGNYNSIAGNGSSECYKCKQPGHYARDCPGQSTGGLECFKCKQPGHFSRDCPVQSTGGSECFKCKQPGHFARDCPGQSTGAQHQTYGNNVAASRGYNRQSFVGGY rfa1a_orysj Replication protein A 70 kDa DNA-binding subunit A RPA1A RPA70A Replication factor A protein 1A Replication protein A 1A OJ1534_E09.2 OSJNBb0013K01.36 OsJ_08582 LOC_Os02g53680 Os02g0776800 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MAMARLTPNGVAAALAGDTNLKPVLQIVELRGVQVNGAGVTRGERFRAVVSDGTAASSALFAAQLSDHARSGALRRGSIVQLSEYVINEVGPRRIIVILNLEVLVSECEIIGNPTALSETGSPIPNPTRVEQFNGAPQYGLMAGNSSNTTTKPSDNVPLFQNSMAGNSSNFATRPSDKVPVFQPTVQPSYRPAPNYKNHGAIMKNEAPARIIPISALNPYQGRWAIKARVTAKGDIRRYHNAKGDGKVFSFDLLDSDGGEIRVTCFNALLDRFYEVVEVGKVYVVSRGNLRPAQKNYNHLNNEWEILLENGSTVDLCPDENSSIPTQRFDFRPINEIEDAQNNAILDIIGVVTSVNPCTTIQRKNGMETQKRTMNLKDMSGRSVEVTMWGDFCNREGSQLQGMVERGIFPVLAVKAGKVSDFSGKSVGTISSTQLFINPDSAEAHSLRQWFDSGGRDASTQSISRDITPGASRNEIRKTVAQIKDEGLGMGDKPDWITVKATVIFFKNESFFYTACPNMIGDRQCNKKVTKSTNGNWTCDKCDREFEECDYRYLLQFQIQDHSGTAWVTAFQEAGQELLGCSATELNALKEREDPRFADTMLNCLFQEYLLRLKVKEESYGDERKVKNTAVKVEKVDPSGESKFLLDLISKSSALH osrpa32-osrpa14 Directed yeast two hybrid analysis reveals interaction between the OsRPA32 protein and OsRPA14 protein 328651 N/A 328654 unspecified role unspecified role prey prey N/A 328655 unspecified role unspecified role bait bait physical association physical association false false false q6h7j5-osrpa14 Directed yeast two hybrid analysis reveals interaction between the Q6H7J5 protein and OsRPA14 protein 328651 N/A 328654 unspecified role unspecified role prey prey N/A 328656 unspecified role unspecified role bait bait physical association physical association false false false q5z8l1-osrpa14 Directed yeast two hybrid analysis reveals interaction between the q5z8l1 protein and OsRPA14 protein 328651 N/A 328654 unspecified role unspecified role prey prey N/A 328657 unspecified role unspecified role bait bait physical association physical association false false false q5z8l1-q65xv7 Directed yeast two hybrid analysis reveals interaction between the q5z8l1 protein and Q65XV7 protein 328651 N/A 328657 unspecified role unspecified role bait bait N/A 328658 unspecified role unspecified role prey prey physical association physical association false false false Very weak interactions between OsRAP32-3 and OsRAP70a and OsRAP70b were detected. q9avm2-osrpa14 Directed yeast two hybrid analysis reveals interaction between the OsRPA32 protein and OsRPA14 protein 328651 N/A 328654 unspecified role unspecified role prey prey N/A 328655 unspecified role unspecified role bait bait physical association physical association false false false Weak interactions between OsRAP32-1 and OsRAP70b or OsRAP70c were detected. q6h7j5-osrpa70a Directed yeast two hybrid analysis reveals interaction between the Q6H7J5 protein and OsRPA70a protein 328651 N/A 328659 unspecified role unspecified role prey prey N/A 328656 unspecified role unspecified role bait bait physical association physical association false false false Weak interactions between Q6H7J5 and Q7Y069 or Q65XV7. q6h7j5-q9avl8-1 Pulldown analysis reveals interaction between the Q6H7J5 protein and Q9AVl8 protein 328652 N/A 328656 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 328659 unspecified role unspecified role prey prey physical association physical association false false false A weak interaction between OsRAP32-2 and OsRAP70c was detected. q5z8l1-q65xv7-1 Pulldown analysis reveals interaction between the q5z8l1 protein and Q65XV7 protein 328652 N/A 328658 unspecified role unspecified role prey prey N/A 328657 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false q9avl8-q6h7j5 Pulldown analysis reveals interaction between the Q9AVl8 protein and Q6H7J5 protein 328652 N/A 328659 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 328656 unspecified role unspecified role prey prey physical association physical association false false false q9avm2-q7y069-1 Pulldown analysis reveals interaction between the OsRPA32 protein and Q7Y069 protein 328652 N/A 328655 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 328654 unspecified role unspecified role prey prey physical association physical association false false false A weak interaction between OsRAP32-1 and OsRAP70c was detected. q65xv7-q5z8l1 Pulldown analysis reveals interaction between the Q65XV7 protein and q5z8l1 protein 328652 N/A 328658 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 328657 unspecified role unspecified role prey prey physical association physical association false false false q7y069-q9avm2 Pulldown analysis reveals interaction between the Q7Y069 protein and OsRPA32 protein 328652 N/A 328655 unspecified role unspecified role prey prey N/A 328654 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false A weak interaction between OsRAP70b and OsRAP32-2 was detected. q9avm2-p0705d01_4-1 Co-immunoprecipitation analysis reveals interaction between the OsRPA32 protein and P0705D01.4 protein 328653 N/A 328655 unspecified role unspecified role prey prey N/A 328654 unspecified role unspecified role bait bait physical association physical association false false false q6h7j5-p0705d01_4-2 Co-immunoprecipitation analysis reveals interaction between the Q6H7J5 protein and P0705D01.4 protein 328653 N/A 328654 unspecified role unspecified role bait bait N/A 328656 unspecified role unspecified role prey prey physical association physical association false false false q5z8l1-p0705d01_4-3 Co-immunoprecipitation analysis reveals interaction between the q5z8l1 protein and P0705D01.4 protein 328653 N/A 328657 unspecified role unspecified role prey prey N/A 328654 unspecified role unspecified role bait bait physical association physical association false false false q6h7j5-q9avl8-2 Co-immunoprecipitation analysis reveals interaction between the Q6H7J5 protein and Q9AVl8 protein 328653 N/A 328656 unspecified role unspecified role prey prey N/A 328659 unspecified role unspecified role bait bait physical association physical association false false false q5z8l1-q65xv7-2 Co-immunoprecipitation analysis reveals interaction between the q5z8l1 protein and Q65XV7 protein 328653 N/A 328657 unspecified role unspecified role prey prey N/A 328658 unspecified role unspecified role bait bait physical association physical association false false false