IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gutsche-2006-1 Biochemical analysis of alginate biosynthesis protein AlgX from Pseudomonas aeruginosa: purification of an AlgX-MucD (AlgY) protein complex. ictbu Ictiobus bubalus (Smallmouth buffalo fish) Catostomus bubalus affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Gutsche J., Remminghorst U., Rehm BH. The cytoplasmic membrane with the non-ionic detergent Triton X-100. Figure 1. B.Rehm@massey.ac.nz Biochimie (0300-9084) 2006 q51415_pseai algY protein protein pseai Pseudomonas aeruginosa MTSPFKVVAVSGGTYRPSRTLVLTQALIAELGQSLPIDSRVIELTDIAAPLGATLARNQAPAELQAVLDEIESADLLLVASPVYRGSYPGLLKHLFDLIDLNALIDTPVLLAATGNTSATRGPRPPVAPAVQLLPGDHPAHRGLRQRGRLRQLPDRQRAAEGAHPPRRRARRAAVRRTLRTVEDRLRCADERVLVPPDPRRWPLPRHQPGRAAGLAVLPEAGGTGGRQPWLSRRTDPHPTLLRRLLGGGLGLAPLTERLRFLVAIRPGIVSPTVSARMTATLDPVRWAPADQRGHRRRSRREPRRRHPFSATPSATRSPTSSSGLAARPAGRGGGLPRQAHPRGERQGALSAAATAVSAAVLRRFLGGRPRTGRRTGGRLPDLGRAVGGGGGENRRRAPASGSPWAYGEVRHPPACDRPRNRRRSLARRGQADRTHLRRNHRRRAAVLRPFRSGRPATHGRAARRPPRSPGDPAEPLGRGRPGARRRRHRAGRRSAAGGGAHRRIRRARHRQLHLLRLSAPGRGLPLRRTGLPAAVRTLRQPGRSWDDQPDRVVRRNDRQRRAAGSRRRLSSRAPEEKPPVNAKTRPEAQTPLQIVRRLAADFAENAAERDVAGGTPKAERDALRRSGLLSLIIPREYGGLGASWSETLQTVRELARVDSSIAHVYGFQHLMLATVRLFSRPEQ algx_pseae Alginate biosynthesis protein AlgX algX Probable alginate O-acetyltransferase AlgX PA3546 protein protein pseae Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) MKTRTSRLFRLSALAAGLCLAQAALAADPGAAPSYQALPAGNLCPAAAYDSRYNTKYLGFFTHLVQAQDDWLFRTTYDLRTDFGTSAEGWRELRALRDELKRKGIELVVVYQPTRGLVNREKLSPAEKAGFDYELAKKNYLATIARFRQAGIWTPDFSPLFDEKEEHAYYFKGDHHWTPHGARRSAKIVAETLKQVPGFEEIPKKQFESKRVGLLSKLGTFHKAAAQLCGNSYATQYVDRFETEPVGASDSGDLFGDGGNPQIALVGTSNSGPAYNFAGFLEEFSGADILNNAVSGGGFDSSLLAYMTSEEFHKNPPKILIWEFATHYDMAQKSFYRQAMPLVDNGCSGRKTVLSRKVKLRQGRNEVLLNSAALPIRSGSYVADVTYSDPSVHELKNTIWYMNGRREQLKIEQSKAVDTGGRYVFQLRNDSDWADQQFLSLEIEAPEDMPQGLEVQASICQAAPAKASQSVAGR algx-algy Affinity chromatograhy assay reveals AlgX interacting proteins. 336447 N/A 336448 unspecified role unspecified role prey prey N/A 336449 unspecified role unspecified role bait bait c terminal Strep-tag II tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false