IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gruhler-2005-1 Stable isotope labeling of Arabidopsis thaliana cells and quantitative proteomics by mass spectrometry. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress pull down pull down ms participant Identification by mass spectrometry ms participant Gruhler A., Schulze WX., Matthiesen R., Mann M., Jensen ON. figure 4 jenseno@bmb.sdu.dk Mol. Cell Proteomics (1535-9476) 2005 14331_arath 14-3-3-like protein GF14 chi General regulatory factor 1 At4g09000 GRF1 F23J3.30 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATPGASSARDEFVYMAKLAEQAERYEEMVEFMEKVAKAVDKDELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDDHVSLIRDYRSKIETELSDICDGILKLLDTILVPAAASGDSKVFYLKMKGDYHRYLAEFKSGQERKDAAEHTLTAYKAAQDIANSELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDDVADDIKEAAPAAAKPADEQQS nia_spiol Nitrate reductase [NADH] NIA protein protein spiol Spinacia oleracea (Spinach) Spinach MAASVDRQYHPAPMSGVVRTPFSNHHRSDSPVRNGYTFSNPPSSNGVVKPGEKIKLVDNNSNSNNGSNNNNNRYDSDSEEDDDENEMNVWNEMIKKGNSELEPSSVDSRDEGTADQWIERNPSMIRLTGKHPFNSEPPLTRLMHHGFLTPVPLHYVRNHGPVPNAKWEDWTVEVTGLVKRPIRFTMDQLVNDFQSREFPVTLVCAGNRRKEQNMTKQSIGFNWGSAAVSTSVWRGVPLRDVLKRCGVMSSLKGALNVCFEGAEDLPGGGGSKYGTSVKREFAMDPARDIILAYMQNGEKLSPDHGYPVRMIIPGFIGGRMVKWLKRIIVTTTESDNYYHYKDNRVLPSHVDAELANSEAWWYKQEYIINELNVNSVITSPCHEEILPINAWTTQRPYTMRGYAYSGGGRKVTRVEVTMDGGDTWDICELDHQERGSKYGKFWCWCFWSLEVEVLDLLGAKEIGVRAWDESLNTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNKSGGWMARERHLEISDSGPTLKRTASTPFMNTTSKMYSMSEVKKHNTADSAWIVVHGNVYNATRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKRLLEDFRIGELISTGYTSDSSSPGNSVHGGSVYSGLAGLAPITEAVPLRNVALNPRVKIPCKLIEKVSLSHDVRRFRFGLPSEDQVLGLPVGKHIFLCANVDDKLCMRAYTPSSTIDVVGYFDLVVKVYFKDVHPRFPNGGVMSQHLDSLSLGSIVDVKGPLGHIEYLGKGNFTVHGKPKFAKKLAMISGGTGITPIYQVMQAILKDPEDKTEMHVVYANRTEEDILLREELDKWADEFRDRVKVWYVVEKAEEGWKYDTGFISEKILRDHVPAVGDDVLALTCGPPPMIQFAVQPNLDKMGFDIKEQLLIF spsa_spiol Sucrose-phosphate synthase UDP-glucose-fructose-phosphate glucosyltransferase SPS1 protein protein spiol Spinacia oleracea (Spinach) Spinach MAGNDWINSYLEAILDVGGQGIDASTGKTSTAPPSLLLRERGHFSPSRYFVEEVISGFDETDLHRSWVRAASTRSPQERNTRLENLCWRIWNLARKKKQIEGEEAQRLAKRHVERERGRREATADMSEDLSEGERGDTVADMLFASESTKGRMRRISSVEMMDNWANTFKEKKLYVVLISLHGLIRGENMELGRDSDTGGQVKYVVELARALGSMPGVYRVDLLTRQVSAPGVDWSYGEPTEMLSSRNSENSTEQLGESSGAYIIRIPFGPKDKYVAKELLWPYIPEFVDGALSHIKQMSKVLGEQIGGGLPVWPASVHGHYADAGDSAALLSGALNVPMVFTGHSLGRDKLDQLLKQGRLSREEVDATYKIMRRIEAEELCLDASEIVITSTRQEIEEQWQLYHGFDLVLERKLRARMRRGVSCHGRFMPRMAKIPPGMEFNHIAPEDADMDTDIDGHKESNANPDPVIWSEIMRFFSNGRKPMILALARPDPKKNLTTLVKAFGECRPLRELANLTLIIGNRDDIDEMSTTSSSVLISILKLIDKYDLYGQVAYPKHHKQSDVPDIYRLAAKTKGVFINPAFIEPFGLTLIEAAAYGLPIVATKNGGPVDIIGVLDNGLLIDPHDQKSIADALLKLVADKHLWTKCRQNGLKNIHLFSWPEHCKNYLSRIASCKPRQPNWQRIDEGSENSDTDSAGDSLRDIQDISLNLKLSLDAERTEGGNSFDDSLDSEEANAKRKIENAVAKLSKSMDKAQVDVGNLKFPAIRRRKCIFVIALDCDVTSDLLQVIKTVISIVGEQRPTGSIGFILSTSMTLSEVDSLLDSGGLRPADFDAFICNSGSELYYPSTDYSESPFVLDQDYYSHIDYRWGGEGLWKTLVKWAASVNEKKGENAPNIVIADETSSTTHCYAFKVNDFTLAPPAKELRKMMRIQALRCHAIYCQNGTRLNVIPVLASRSQALRYLFMRWGVELSNFVVFVGESGDTDYEGLLGGVHKTVILKGIGSNTSNFHATRAYPMEHVMPVDSPNMFQTGGCNIDDISDALSKIGCLKAQKSL nia1-grf1 336797 N/A 336798 unspecified role unspecified role prey prey N/A 336799 unspecified role unspecified role bait bait region synthetic phosphopeptide of this sequence was used for pull-down experiment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region phosphorylation required for binding opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Sequence coverage of 14-3-3 protein not sufficient to distinguish between different isoforms sps1-grf1 336797 N/A 336798 unspecified role unspecified role prey prey N/A 336800 unspecified role unspecified role bait bait region synthetic phosphopeptide used for pull-down sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region phosphorylation required for binding opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Sequence coverage of 14-3-3 protein not sufficient to distinguish between different isoforms