UniProt UniProt http://www.uniprot.org mccleland-2003-1 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Genes Dev. (0890-9369) 2003 mccleland-2003-2 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. xenla-oocyte Xenopus laevis oocyte Oocyte cosedimentation cosedimentation polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Genes Dev. (0890-9369) 2003 mccleland-2003-3 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. xenla-oocyte Xenopus laevis oocyte Oocyte molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. A superose 6 column was used. pts7h@virginia.edu Genes Dev. (0890-9369) 2003 mccleland-2003-4 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. xenla Xenopus laevis (African clawed frog) African clawed frog fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Genes Dev. (0890-9369) 2003 ndc80_xenla Kinetochore protein NDC80 homolog kntc2 Kinetochore protein Hec1 Kinetochore-associated protein 2 ndc80 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRRSSVTNRQSLLPLRVQDANRMGLTTPQNKDRQGFGKLSMSKPHSGTSEKKTSFFGKRASNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQALTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSENPVFEDPPMGEQSENGIDFNQLFLDYTVRCYDQFMEGRDTFEEYDTDVCIRLKEAYHVDESNLEALQQESRRLMEEIERLEKEKENEPDRLASMRKLKASLQADIQKYQNYLTEIESHSTLLDQRVSSISEELEATELESRAIQQENLRLKNILDNQKYSVADIERIKYEETELQQTIAKLTKDLDEDKQQLWHEELKYAKMKESVETQLSEFHKIGRKVRLIPPTAEFANGYDFQIQCNLDSEQSSLMHYRNKINVPLVEILSQSEGHIASSTNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKLEEIYQQKVEESEEHEKRWISEIESLEKHRQLLESGVNKSLDEAMKDLQKEQQELQLVEHRTEEEMRQVENKLVRVVHAVANHVAVIEKHLEEKRLKVEREYEEFMKEDLLLDLRELLEKYKEKARVLDSL nuf2a_xenla Kinetochore protein Nuf2-A Cell division cycle-associated protein 1-A nuf2-a cdca1-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDKLTFPIFPAADLVNFFRQNILTGTEAKNFNKNDIFPNPKPEMVQKLYMRILQQVFNYGVEQFYMVPMDLDIQYPHLVEGFAPVANILKLMARFLPMCRVYDFHPSDVLNPKGKRTLHSLSGIVNFLHFSATRKEVYFEYCSSYKSALENVRQLQKANQEAEIKIEKLTTVPPEQQAEFKALSSEIHDLQQIISQEYRAKDVAFQEKIAQRKTEFAEKNKRLNEQKLAIATMKEEQERMKSQIVESPEQRKSKTERMKETVHRLKQARQETNDKCDYYRDRVAFACMWQTDVQGYLKKLQGIDTNLEIHRKIREEIRHSEEQVVNLNLELKSLSNEDAQLKRIILVKKEKLAKVDIKNKKKQEDFNQQKQEILEVCSRIQEKRQVVHGRVAQVLQEIQQTIGKKEQLLETTEAGKKKCQEVITDFRAALEKYHDSLQKASERSADRRREKIAELNRRLSRR kntc2-cdca1-7 coIP: bait=xNdc80, prey=xNuf2 507681 N/A 507685 unspecified role unspecified role bait bait N/A 507686 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B cdca1-kntc2-2 coIP: bait=xNuf2, prey=xNdc80 507681 N/A 507686 unspecified role unspecified role bait bait N/A 507685 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B kntc2-cdca1-8 cosedimentation of xNdc80 and xNuf2 during sucrose gradient centrifugation 507682 N/A 507685 unspecified role unspecified role neutral component neutral component N/A 507686 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1C kntc2-cdca1-9 comigration of xNdc80 and xNuf2 during superose 6 gel filtration 507683 N/A 507686 unspecified role unspecified role neutral component neutral component N/A 507685 unspecified role unspecified role neutral component neutral component physical association physical association false false false Supplementary Fig. 2A kntc2-cdca1-10 colocalization of xNdc80 and xNuf2 507684 N/A 507685 unspecified role unspecified role neutral component neutral component N/A 507686 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supplementary Fig. 2D