UniProt UniProt http://www.uniprot.org park-2002-1 Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid partial dna sequence hybrid partial dna sequence identification by hybridization partial dna sequence hybrid Park YU., Hwang O., Kim J. jmkim@cnu.ac.kr Biochem. Biophys. Res. Commun. (0006-291X) 2002 Only protein-protein interactions imex curation park-2002-2 Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Park YU., Hwang O., Kim J. jmkim@cnu.ac.kr Biochem. Biophys. Res. Commun. (0006-291X) 2002 Only protein-protein interactions imex curation osh3_yeast Oxysterol-binding protein homolog 3 YHR073W OSH3 protein protein yeast Saccharomyces cerevisiae Baker's yeast METIDIQNRSFVVRWVKCGRGDVINYQIKPLKKSIEVGIYKKLKSSVDDHASAVHIAPDTKTLLDYTTKSLLHKGSSSNIEEHHRRSSQHSHSSSNGSDNKRKERSYSSLSISGIQQQSQEIPLREKLSASGFTLVKRVGNVSGNTMVQGDLEVKDTDYYYAFILDNSSSKNAKKKILFNASVINGDNQSMISTRSTPPARPTALSRTSTQQDMLFRVGQGRYLQGYLLKKRRKRLQGFKKRFFTLDFRYGTLSYYLNDHNQTCRGEIVISLSSVSANKKDKIIIIDSGMEVWVLKATTKENWQSWVDALQTCFDDQFEDKDTSTLEENPDILDDDKEVINKSSPQDHDHLTPTATTKSALSHRQHTQKDMDDIYVPLPSESYATFSMNLRLIQQRLEQCKKDSLSYKPTTLHQRSEGLNGTHSSSSVFTNNRVSSFNHSSSGMTSSDSLASEEVPSNKTYIEHALYNQLADLEVFVSRFVTQGEVLFKDHQILCKKAKDTRVSLTSYLSENDEFFDAEEEISRGVIILPDTEDDINNIVEETPLLGKSDQNEFTKEVQLSGSEQIASSSVESYTTNDENHSRKHLKNRHKNRRRGHPHHQKTKSAQSSTETFTSKDLFALSYPKSVTRRNDIPEAAASPPSLLSFLRKNVGKDLSSIAMPVTSNEPISILQLISETFEYAPLLTKATQRPDPITFVSAFAISFLSIYRDKTRTLRKPFNPLLAETFELIREDMGFRLISEKVSHRPPVFAFFAEHLDWECSYTVTPSQKFWGKSIELNNEGILRLKFKTTGELFEWTQPTTILKNLIAGERYMEPVNEFEVHSSKGDKSHILFDKAGMFSGRSEGFKVSIIPPPSSNRKKETLAGKWTQSLANETTHETIWEVGDLVSNPKKKYGFTKFTANLNEITEIEKGNLPPTDSRLRPDIRAYEEGNVDKAEEWKLKLEQLQRERRNKGQDVEPKYFEKVSKNEWKYITGPKSYWERRKKHDWSDISQLW B1AD1210EFFB9AFE rok1_yeast ATP-dependent RNA helicase ROK1 ROK1 YGL171W G1651 Rescuer of KEM1 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQIVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRKSYKGNVSGIDIPLPIGSFEDLISRFSFDKRLLNNLIENGFTEPTPIQCECIPVALNNRDVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYKIFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKHLIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVIIGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHELMYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVPGSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKMTRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK CACB950B8A15D5A2 fal1_yeast ATP-dependent RNA helicase FAL1 FAL1 YDR021W YD9335.07 PZC399 Translation initiation factor four A-like protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFDREEDQKLKFKTSKKLKVSSTFESMNLKDDLLRGIYSYGFEAPSSIQSRAITQIISGKDVIAQAQSGTGKTATFTIGLLQAIDLRKKDLQALILSPTRELASQIGQVVKNLGDYMNVNAFAITGGKTLKDDLKKMQKHGCQAVSGTPGRVLDMIKKQMLQTRNVQMLVLDEADELLSETLGFKQQIYDIFAKLPKNCQVVVVSATMNKDILEVTRKFMNDPVKILVKRDEISLEGIKQYVVNVDKEEWKFDTLCDIYDSLTITQCVIFCNTKKKVDWLSQRLIQSNFAVVSMHGDMKQEERDKVMNDFRTGHSRVLISTDVWARGIDVQQVSLVINYDLPEIIENYIHRIGRSGRFGRKGVAINFITKADLAKLREIEKFYSIKINPMPANFAELS 76A3441728D21253 rok1-osh3 interaction detected by Y2H 495077 N/A 495079 unspecified role unspecified role prey prey N/A 495080 unspecified role unspecified role bait bait c-terminal C-terminal region binding to OSH3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 rok1-fal1 interaction detected by Y2H 495077 N/A 495080 unspecified role unspecified role bait bait n-terminal N-terminal region binding to FAL1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 495081 unspecified role unspecified role prey prey physical association physical association false false false 1 rok1-osh3-2 interaction detected by Y2H 495077 N/A 495080 unspecified role unspecified role bait bait N/A 495079 unspecified role unspecified role prey prey physical association physical association false false false 1 rok1-osh3-1 interaction detected by CoIP 495078 N/A 495080 unspecified role unspecified role prey prey N/A 495079 unspecified role unspecified role bait bait c-terminus C-terminus HA tagged protein OSH3 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2B