UniProt UniProt http://www.uniprot.org measday-2002-1 Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Measday V., Hailey DW., Pot I., Givan SA., Hyland KM., Cagney G., Fields S., Davis TN., Hieter P. hieter@cmmt.ubc.ca Genes Dev. (0890-9369) 2002 Only protein-protein interactions imex curation measday-2002-2 Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Measday V., Hailey DW., Pot I., Givan SA., Hyland KM., Cagney G., Fields S., Davis TN., Hieter P. hieter@cmmt.ubc.ca Genes Dev. (0890-9369) 2002 Only protein-protein interactions imex curation cenpk_yeast Inner kinetochore subunit MCM22 YJR135C J2122 MCM22 Minichromosome maintenance protein 22 CENP-K homolog Constitutive centromere-associated network protein MCM22 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDVEKDVLDVYIKNLENQIGNKRYFLKQAQGAIDEITKRSLDTEGKPVNSEVFTELLRKPMFFSERADPIGFSLTSNFLSLRAQSSSEWLSLMNDQSVDQKAMLLLQNNINSDLKELLRKLQHQMTIMDSKKQDHAHIRTRKARNKELWDSLADFLKGYLVPNLDDNDESIDSLTNEVMLLMKRLIEHDLNLTLNDFSSKTIPIYRLLLRANIITVIEGSTNPGTKYIKLIDFNETSLT cenpi_yeast Inner kinetochore subunit CTF3 Chromosome transmission fidelity protein 3 Chromosome loss protein 3 CTF3 CHL3 CENP-I homolog Constitutive centromere-associated network protein CTF3 YLR381W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLILDDIILSLTNANERTPPQALKTTLSLLYEKSKQYGLSSPQLQALVRLLCETSIIDTVTKVYIVENCFLPDGYLTKELLLEIINHLGTPTVFSRYRIQTPPVLQSALCKWLVHVYFLFPVHSEREHNISSSIWLHLWQFSFLQKWITPLVIWQATTPVDVKPWKLSIIKRCAMHPGYRDAPGSATLILQRFQCLVGASSQITESIITINCNRKTLKSHRNLKLDAHFLSILKRILSRAHPANFPADTVQNTIDMYLSEIHQLGADSIYPLRLQSLPEYVPSDSTVSLWDVTSLEQLAQNWPQLHIPNDVDYMMKPSLNSNVLLPRKVMSRDSLKHLYSSIILIKNSRDESSSPYEWCIWQLKRCFAHQIETPQEVIPIIISVSSMDNKLSSRIIQTFCNLKYLKLDELTLKKVCGGILPLWKPELISGTREFFVKFMASIFMWSTRDGHDNNCTFSETCFYVLQMITNWVLDDKLIALGLTLLHDMQSLLTLDKIFNNATSNRFSTMAFISSLDILTQLSKQTKSDYAIQYLIVGPDIMNKVFSSDDPLLLSAACRYLVATKNKLMQYPSTNKFVRMQNQYIMDLTNYLYRNKVLSSKSLFGVSPDFFKQILENLYIPTADFKNAKFFTITGIPALSYICIIILRRLETAENTKIKFTSGIINEETFNNFFRVHHDEIGQHGWIKGVNNIHDLRVKILMHLSNTANPYRDIAAFLFTYLKSLSKYSVQNS cenph_yeast Inner kinetochore subunit MCM16 Minichromosome maintenance protein 16 MCM16 YPR046W CENP-H homolog Constitutive centromere-associated network protein MCM16 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTNSSEKQWERIQQLEKEHVEVYRELLITLDRLYLIRKHNHAVILSHTQQRLLEIRHQLQINLEKTALLIRLLEKPDNTNVLFTKLQNLLEESNSLDYELLQSLGAQSSLHKQLIESRAERDELMSKLIELSSKFPKPTIPPDDSDTAGKQVEVEKENETIQELMIALQIHSGYTNISYTI cenpp_yeast Inner kinetochore subunit CTF19 CTF19 YPL018W LPB13W MCM18 Chromosome transmission fidelity protein 19 Minichromosome maintenance protein 18 CENP-P homolog Constitutive centromere-associated network protein CTF19 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDFTSDTTNSHDTSNSHLSLEDAVGTHHAGEADVNIDGDEKQQLSLLDDDQVRALKLQEEKDALLTRRNTLLQEIQTYQNILMKENNSKTKNGDILQNDITQDFLNLISISSSNPNSAISDRKRVERINGLTNLQKELVTKYDTLPLLNMNLRLSYLRDHTYPHLQVSVQSRDRVHNDGIEVLVVNYKFCRNTMNPFEIQFKMFYKFEDSTLLKWEILRISTNVRLKAKQLLATRNFQKCLLSLYEFDKIKSKKTGIFQNLINLLKRKTRCYLMNNSDSLIVERVIREGRLTTIKLQINFIITMPGERGKPRNCFLPMSKISIALWKGGERFNQIDLDEICYGLIKEYGVKTGLKEICNVCLFPDMYAR 23B4CBD6AE26E793 spc34_yeast DASH complex subunit SPC34 YKR037C SPC34 34 kDa spindle pole component protein Outer kinetochore protein SPC34 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGESLDRCIDDINRAVDSMSTLYFKPPGIFHNAILQGASNKASIRKDITRLIKDCNHDEAYLLFKVNPEKQSVSRRDGKEGVFDYVIKRDTDMKRNRRLGRPGEKPIIHVPKEVYLNKDRLDLNNKRRRTATTSGGGLNGFIFDTDLIGSSVISNSSSGTFKALSAVFKDDPQIQRLLYALENGSVLMEEESNNQRRKTIFVEDFPTDLILKVMAEVTDLWPLTEFKQDYDQLYHNYEQLSSKLRFIKKEVLLQDDRLKTMSQYHPSSSHDVAKIIRKEKDEIRRLEMEIANLQE C1061B49F6D4D308 ctf3-mcm22 Interaction detected by coIP 494403 N/A 494405 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged protein MCM22 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag monoclonal western monoclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false N/A 494406 unspecified role unspecified role bait bait c-terminus C-terminus MYC tagged protein CTF3 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2A ctf3-mcm16 Interaction detected by coIP 494403 N/A 494406 unspecified role unspecified role bait bait c-terminus C-terminus MYC tagged protein CTF3 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 494407 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged protein MCM16 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2C mcm22-mcm16 Interaction detected by coIP 494403 N/A 494405 unspecified role unspecified role bait bait c-terminus C-terminus MYC tagged protein MCM22 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 494407 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged protein MCM16 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2B mcm22-ctf9 Interaction detected by coIP 494403 N/A 494405 unspecified role unspecified role bait bait c-terminus C-terminus MYC tagged protein MCM22 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 494408 unspecified role unspecified role prey prey physical association physical association false false false 2D ctf3-ctf9 Interaction detected by coIP 494403 N/A 494406 unspecified role unspecified role bait bait c-terminus C-terminus Myc tagged protein CTF3 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 494408 unspecified role unspecified role prey prey physical association physical association false false false 2D mcm16-ctf9 Interaction detected by coIP 494403 N/A 494407 unspecified role unspecified role bait bait c-terminus C-terminus MYC tagged protein MCM16 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 494408 unspecified role unspecified role prey prey physical association physical association false false false 2D ctf3-mcm22-1 Interaction detected by Y2H 494404 N/A 494405 unspecified role unspecified role prey prey N/A 494406 unspecified role unspecified role bait bait physical association physical association false false false None mcm22-spc34 Interaction detected by Y2H 494404 N/A 494405 unspecified role unspecified role bait bait N/A 494409 unspecified role unspecified role prey prey physical association physical association false false false None