IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-1997-1 Protein-protein interactions among the Aux/IAA proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim J., Harter K., Theologis A. Table 1. Theo@Nature.Berkeley.Edu The lambda-ACT cDNA library used in this study was constructed from mRNA extracted from 3 day old etiolated arabidopsis seedlings. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 kim-1997-2 Protein-protein interactions among the Aux/IAA proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim J., Harter K., Theologis A. Directed yeast two hybrid assay. Table 1. Theo@Nature.Berkeley.Edu The lambda-ACT cDNA library used in this study was constructed from mRNA extracted from 3 day old etiolated arabidopsis seedlings. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 kim-1997-3 Protein-protein interactions among the Aux/IAA proteins. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Kim J., Harter K., Theologis A. Gel filtration followed by chemical cross linking was used in this study. Bi-sulfosuccinimidyl was used as a cross linking reagent in this study. Table 1. Theo@Nature.Berkeley.Edu The lambda-ACT cDNA library used in this study was constructed from mRNA extracted from 3 day old etiolated arabidopsis seedlings. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 iaa1_arath Auxin-responsive protein IAA1 Indoleacetic acid-induced protein 1 IAA1 At4g14560 dl3320w FCAALL.409 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEVTNGLNLKDTELRLGLPGAQEEQQLELSCVRSNNKRKNNDSTEESAPPPAKTQIVGWPPVRSNRKNNNNKNVSYVKVSMDGAPYLRKIDLKMYKNYPELLKALENMFKFTVGEYSEREGYKGSGFVPTYEDKDGDWMLVGDVPWDMFSSSCQKLRIMKGSEAPTAL iaa3_arath Auxin-responsive protein IAA3 IAA3 SHY2 At1g04240 F19P19.32 Indoleacetic acid-induced protein 3 Short hypocotyl Suppressor of HY2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEFVNLKETELRLGLPGTDNVCEAKERVSCCNNNNKRVLSTDTEKEIESSSRKTETSPPRKAQIVGWPPVRSYRKNNIQSKKNESEHEGQGIYVKVSMDGAPYLRKIDLSCYKGYSELLKALEVMFKFSVGEYFERDGYKGSDFVPTYEDKDGDWMLIGDVPWEMFICTCKRLRIMKGSEAKGLGCGV iaa4_arath Auxin-responsive protein IAA4 Indoleacetic acid-induced protein 4 Auxin-induced protein AUX2-11 IAA4 AUX2-11 At5g43700 MQD19.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKVDVYDELVNLKATELRLGLPGTEETVSCGKSNKRVLPEATEKEIESTGKTETASPPKAQIVGWPPVRSYRKNNVQTKKSESEGQGNYVKVSMDGAPYLRKIDLTMYKQYPELMKSLENMFKFSVGEYFEREGYKGSDFVPTYEDKDGDWMLVGDVPWEMFVSSCKRLRIMKGSEVKGLGCGGL q38826-1 Auxin-responsive protein IAA8 IAA8 Indoleacetic acid-induced protein 8 T9I22.11 At2g22670 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSYRLLSVDKDELVTSPCLKERNYLGLSDCSSVDSSTIPNVVGKSNLNFKATELRLGLPESQSPERETDFGLLSPRTPDEKLLFPLLPSKDNGSATTGHKNVVSGNKRGFADTWDEFSGVKGSVRPGGGINMMLSPKVKDVSKSIQEERSHAKGGLNNAPAAKAQVVGWPPIRSYRKNTMASSTSKNTDEVDGKPGLGVLFVKVSMDGAPYLRKVDLRTYTSYQQLSSALEKMFSCFTLGQCGLHGAQGRERMSEIKLKDLLHGSEFVLTYEDKDGDWMLVGDVPWEIFTETCQKLKIMKGSDSIGLAPGAVEKSKNKERV iaa9_arath Auxin-responsive protein IAA9 Indoleacetic acid-induced protein 9 IAA9 At5g65670 F6H11.210 MPA24.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSPEEELQSNVSVASSSPTSNCISRNTLGGLKEHNYLGLSDCSSVGSSTLSPLAEDDKATISLKATELTLGLPGSQSPARDTELNLLSPAKLDEKPFFPLLPSKDEICSSSQKNNASGNKRGFSDTMDQFAEAKSSVYTEKNWMFPEAAATQSVTKKDVPQNIPKGQSSTTNNSSSPPAAKAQIVGWPPVRSYRKNTLATTCKNSDEVDGRPGSGALFVKVSMDGAPYLRKVDLRSYTNYGELSSALEKMFTTFTLGQCGSNGAAGKDMLSETKLKDLLNGKDYVLTYEDKDGDWMLVGDVPWEMFIDVCKKLKIMKGCDAIGLAAAPRAMEKSKMRA iaa16_arath Auxin-responsive protein IAA16 Indoleacetic acid-induced protein 16 IAA16 At3g04730 F7O18.22 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MINFEATELRLGLPGGNHGGEMAGKNNGKRGFSETVDLKLNLSSTAMDSVSKVDLENMKEKVVKPPAKAQVVGWPPVRSFRKNVMSGQKPTTGDATEGNDKTSGSSGATSSASACATVAYVKVSMDGAPYLRKIDLKLYKTYQDLSNALSKMFSSFTIGNYGPQGMKDFMNESKLIDLLNGSDYVPTYEDKDGDWMLVGDVPWEMFVDSCKRIRIMKGSEAIGLAPRALEKCKNRS iaa17_arath Auxin-responsive protein IAA17 Indoleacetic acid-induced protein 17 Auxin response 3 IAA17 AXR3 At1g04250 F19P19.31 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEKCKSRA iaa19_arath Auxin-responsive protein IAA19 Indoleacetic acid-induced protein 19 Protein MASSUGU 2 IAA19 MSG2 At3g15540 MJK13.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKEGLGLEITELRLGLPGRDVAEKMMKKRAFTEMNMTSSGSNSDQCESGVVSSGGDAEKVNDSPAAKSQVVGWPPVCSYRKKNSCKEASTTKVGLGYVKVSMDGVPYLRKMDLGSSQGYDDLAFALDKLFGFRGIGVALKDGDNCEYVTIYEDKDGDWMLAGDVPWGMFLESCKRLRIMKRSDATGFGLQPRGVDE iaa20_arath Auxin-responsive protein IAA20 Indoleacetic acid-induced protein 20 IAA20 At2g46990 F14M4.18 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRGRSSSSSSIESSSKSNPFGASSSTRNLSTDLRLGLSFGTSSGTQYFNGGYGYSVAAPAVEDAEYVAAVEEEEENECNSVGSFYVKVNMEGVPIGRKIDLMSLNGYRDLIRTLDFMFNASILWAEEEDMCNEKSHVLTYADKEGDWMMVGDVPWEMFLSTVRRLKISRANYHY arfg_arath Auxin response factor 7 Auxin-responsive protein IAA21/IAA23/IAA25 TIR5 Protein BIPOSTO ARF7 BIP IAA21 IAA23 IAA25 NPH4 At5g20730 T1M15.130 Protein NON-PHOTOTROPIC HYPOCOTYL 4 Protein TRANSPORT INHIBITOR RESPONSE 5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKAPSSNGVSPNPVEGERRNINSELWHACAGPLISLPPAGSLVVYFPQGHSEQVAASMQKQTDFIPSYPNLPSKLICMLHNVTLNADPETDEVYAQMTLQPVNKYDRDALLASDMGLKLNRQPNEFFCKTLTASDTSTHGGFSVPRRAAEKIFPALDFSMQPPCQELVAKDIHDNTWTFRHIYRGQPKRHLLTTGWSVFVSTKRLFAGDSVLFIRDGKAQLLLGIRRANRQQPALSSSVISSDSMHIGVLAAAAHANANNSPFTIFYNPRAAPAEFVVPLAKYTKAMYAQVSLGMRFRMIFETEECGVRRYMGTVTGISDLDPVRWKNSQWRNLQIGWDESAAGDRPSRVSVWDIEPVLTPFYICPPPFFRPRFSGQPGMPDDETDMESALKRAMPWLDNSLEMKDPSSTIFPGLSLVQWMNMQQQNGQLPSAAAQPGFFPSMLSPTAALHNNLGGTDDPSKLLSFQTPHGGISSSNLQFNKQNQQAPMSQLPQPPTTLSQQQQLQQLLHSSLNHQQQQSQSQQQQQQQQLLQQQQQLQSQQHSNNNQSQSQQQQQLLQQQQQQQLQQQHQQPLQQQTQQQQLRTQPLQSHSHPQPQQLQQHKLQQLQVPQNQLYNGQQAAQQHQSQQASTHHLQPQLVSGSMASSVITPPSSSLNQSFQQQQQQSKQLQQAHHHLGASTSQSSVIETSKSSSNLMSAPPQETQFSRQVEQQQPPGLNGQNQQTLLQQKAHQAQAQQIFQQSLLEQPHIQFQLLQRLQQQQQQQFLSPQSQLPHHQLQSQQLQQLPTLSQGHQFPSSCTNNGLSTLQPPQMLVSRPQEKQNPPVGGGVKAYSGITDGGDAPSSSTSPSTNNCQISSSGFLNRSQSGPAILIPDAAIDMSGNLVQDLYSKSDMRLKQELVGQQKSKASLTDHQLEASASGTSYGLDGGENNRQQNFLAPTFGLDGDSRNSLLGGANVDNGFVPDTLLSRGYDSQKDLQNMLSNYGGVTNDIGTEMSTSAVRTQSFGVPNVPAISNDLAVNDAGVLGGGLWPAQTQRMRTYTKVQKRGSVGRSIDVNRYRGYDELRHDLARMFGIEGQLEDPQTSDWKLVYVDHENDILLVGDDPWEEFVNCVQSIKILSSAEVQQMSLDGNFAGVPVTNQACSGGDSGNAWRGHYDDNSATSFNR arfs_arath Auxin response factor 19 Auxin-responsive protein IAA22 ARF19 IAA22 At1g19220 T29M8.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKAPSNGFLPSSNEGEKKPINSQLWHACAGPLVSLPPVGSLVVYFPQGHSEQVAASMQKQTDFIPNYPNLPSKLICLLHSVTLHADTETDEVYAQMTLQPVNKYDREALLASDMGLKLNRQPTEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFSMQPPAQEIVAKDLHDTTWTFRHIYRGQPKRHLLTTGWSVFVSTKRLFAGDSVLFVRDEKSQLMLGIRRANRQTPTLSSSVISSDSMHIGILAAAAHANANSSPFTIFFNPRASPSEFVVPLAKYNKALYAQVSLGMRFRMMFETEDCGVRRYMGTVTGISDLDPVRWKGSQWRNLQVGWDESTAGDRPSRVSIWEIEPVITPFYICPPPFFRPKYPRQPGMPDDELDMENAFKRAMPWMGEDFGMKDAQSSMFPGLSLVQWMSMQQNNPLSGSATPQLPSALSSFNLPNNFASNDPSKLLNFQSPNLSSANSQFNKPNTVNHISQQMQAQPAMVKSQQQQQQQQQQHQHQQQQLQQQQQLQMSQQQVQQQGIYNNGTIAVANQVSCQSPNQPTGFSQSQLQQQSMLPTGAKMTHQNINSMGNKGLSQMTSFAQEMQFQQQLEMHNSSQLLRNQQEQSSLHSLQQNLSQNPQQLQMQQQSSKPSPSQQLQLQLLQKLQQQQQQQSIPPVSSSLQPQLSALQQTQSHQLQQLLSSQNQQPLAHGNNSFPASTFMQPPQIQVSPQQQGQMSNKNLVAAGRSHSGHTDGEAPSCSTSPSANNTGHDNVSPTNFLSRNQQQGQAASVSASDSVFERASNPVQELYTKTESRISQGMMNMKSAGEHFRFKSAVTDQIDVSTAGTTYCPDVVGPVQQQQTFPLPSFGFDGDCQSHHPRNNLAFPGNLEAVTSDPLYSQKDFQNLVPNYGNTPRDIETELSSAAISSQSFGIPSIPFKPGCSNEVGGINDSGIMNGGGLWPNQTQRMRTYTKVQKRGSVGRSIDVTRYSGYDELRHDLARMFGIEGQLEDPLTSDWKLVYTDHENDILLVGDDPWEEFVNCVQNIKILSSVEVQQMSLDGDLAAIPTTNQACSETDSGNAWKVHYEDTSAAASFNR arfe_arath Auxin response factor 5 Transcription factor MONOPTEROS Auxin-responsive protein IAA24 ARF5 IAA24 MP At1g19850 F6F9.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMASLSCVEDKMKTSCLVNGGGTITTTTSQSTLLEEMKLLKDQSGTRKPVINSELWHACAGPLVCLPQVGSLVYYFSQGHSEQVAVSTRRSATTQVPNYPNLPSQLMCQVHNVTLHADKDSDEIYAQMSLQPVHSERDVFPVPDFGMLRGSKHPTEFFCKTLTASDTSTHGGFSVPRRAAEKLFPPLDYSAQPPTQELVVRDLHENTWTFRHIYRGQPKRHLLTTGWSLFVGSKRLRAGDSVLFIRDEKSQLMVGVRRANRQQTALPSSVLSADSMHIGVLAAAAHATANRTPFLIFYNPRACPAEFVIPLAKYRKAICGSQLSVGMRFGMMFETEDSGKRRYMGTIVGISDLDPLRWPGSKWRNLQVEWDEPGCNDKPTRVSPWDIETPESLFIFPSLTSGLKRQLHPSYFAGETEWGSLIKRPLIRVPDSANGIMPYASFPSMASEQLMKMMMRPHNNQNVPSFMSEMQQNIVMGNGGLLGDMKMQQPLMMNQKSEMVQPQNKLTVNPSASNTSGQEQNLSQSMSAPAKPENSTLSGCSSGRVQHGLEQSMEQASQVTTSTVCNEEKVNQLLQKPGASSPVQADQCLDITHQIYQPQSDPINGFSFLETDELTSQVSSFQSLAGSYKQPFILSSQDSSAVVLPDSTNSPLFHDVWDTQLNGLKFDQFSPLMQQDLYASQNICMSNSTTSNILDPPLSNTVLDDFCAIKDTDFQNHPSGCLVGNNNTSFAQDVQSQITSASFADSQAFSRQDFPDNSGGTGTSSSNVDFDDCSLRQNSKGSSWQKIATPRVRTYTKVQKTGSVGRSIDVTSFKDYEELKSAIECMFGLEGLLTHPQSSGWKLVYVDYESDVLLVGDDPWEEFVGCVRCIRILSPTEVQQMSEEGMKLLNSAGINDLKTSVS iaa2_arath Auxin-responsive protein IAA2 Indoleacetic acid-induced protein 2 IAA2 At3g23030 MXC7.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAYEKVNELNLKDTELCLGLPGRTEKIKEEQEVSCVKSNNKRLFEETRDEEESTPPTKTQIVGWPPVRSSRKNNNSVSYVKVSMDGAPYLRKIDLKTYKNYPELLKALENMFKVMIGEYCEREGYKGSGFVPTYEDKDGDWMLVGDVPWDMFSSSCKRLRIMKGSDAPALDSSL iaa4_pea Auxin-induced protein IAA4 IAA4/5 protein protein pea Pisum sativum (Garden pea) Garden pea MEFKATELRLGLPGITEEEEKKIIHGSSVVKNNNKRQLPQTSEESVSISKVTNDEHIVESSSAAPPAKAKIVGWPPIRSYRKNSLHEADVGGIFVKVSMDGAPYLRKIDLRVYGGYSELLKALETMFKLTIGEYSEREGYKGSEYAPTYEDKDGDWMLVGDVPWDMFVTSCKRLRIMKGTEAKGLGCGV iaa1-iaa3 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA3 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207155 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa4 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA4 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207156 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa8 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA8 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207157 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa9 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA9 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207158 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa16 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA16 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207159 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa17 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA17 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207160 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa19 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA19 protein 207151 N/A 207161 unspecified role unspecified role prey prey N/A 207154 unspecified role unspecified role bait bait physical association physical association false false false iaa1-iaa20 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA20 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207162 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa21 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA21 protein 207151 N/A 207154 unspecified role unspecified role bait bait N/A 207163 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa22 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA22 protein 207151 N/A 207164 unspecified role unspecified role prey prey N/A 207154 unspecified role unspecified role bait bait physical association physical association false false false iaa1-iaa24 Yeast 2 hybrid analysis reveals interaction between IAA1 protein and the IAA24 protein 207151 N/A 207165 unspecified role unspecified role prey prey N/A 207154 unspecified role unspecified role bait bait physical association physical association false false false iaa1-iaa1 Directed yeast-2-hybrid reveals homotypic interaction of the IAA1 protein. 207152 N/A 207154 unspecified role unspecified role prey prey N/A 207154 unspecified role unspecified role bait bait physical association physical association false false false iaa2-iaa2 Directed yeast-2-hybrid reveals homotypic interaction of the IAA2 protein. 207152 N/A 207166 unspecified role unspecified role prey prey N/A 207166 unspecified role unspecified role bait bait physical association physical association false false false iaa4-iaa4 Directed yeast-2-hybrid reveals homotypic interaction of the IAA4 protein. 207152 N/A 207167 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 207167 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false iaa1-iaa2 Directed yeast-2-hybrid reveals interaction between IAA1 protein and the IAA2 protein. 207152 N/A 207154 unspecified role unspecified role bait bait N/A 207166 unspecified role unspecified role prey prey physical association physical association false false false iaa2-iaa1 Directed yeast-2-hybrid reveals interaction between IAA2 protein and the IAA1 protein. 207152 N/A 207154 unspecified role unspecified role prey prey N/A 207166 unspecified role unspecified role bait bait physical association physical association false false false iaa1-iaa4-1 Directed yeast-2-hybrid reveals interaction between IAA1 protein and the IAA4 protein. 207152 N/A 207154 unspecified role unspecified role bait bait N/A 207167 unspecified role unspecified role prey prey physical association physical association false false false iaa4-iaa1 Directed yeast-2-hybrid reveals interaction between IAA4 protein and the IAA1 protein. 207152 N/A 207167 unspecified role unspecified role bait bait N/A 207154 unspecified role unspecified role prey prey physical association physical association false false false iaa1-iaa4-2 Chemical crosslinking analysis reveals interaction between IAA1 protein and the IAA4 protein 207153 N/A 207154 unspecified role unspecified role neutral component neutral component his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 207167 unspecified role unspecified role neutral component neutral component his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false