UniProt UniProt http://www.uniprot.org blower-2005-1 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Cell (0092-8674) 2005 blower-2005-2 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. xenla-oocyte Xenopus laevis oocyte Oocyte coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Cell (0092-8674) 2005 blower-2005-3 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Cell (0092-8674) 2005 blower-2005-4 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. xenla-oocyte Xenopus laevis oocyte Oocyte coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Cell (0092-8674) 2005 q7zzy7_xenla Rae1 protein homolog rae1.S Gle2 Rae1 mRNA export factor mRNA-associated protein mrnp 41 XELAEV_18046216mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTLFSTPGGFGTGGGSMFGSTATDNHNPMKDIEVASPPDDSISCLSFSPQTLPGNFLIAGSWANDVRCWEVQDNGQTIPKAQQMHTGPVQDVCWSDDGTKVFTASCDKTAKMWDLNSNQSIQIAQHDAPIKTVHWVKAPNYSCIMTGSWDKTLKFWDTRSPNPLLSIQLPERGYCADVVYPMAVVATAERGLIVYQLENQPSEFRRIDSPLKHQHRCVGIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTAAPQDIYAVNDIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPITACCFNHNGNIFAYSSSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK imb_xenla Importin subunit beta p97 Importin-90 Karyopherin subunit beta kpnb1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELVTILEKTVSPDRNELEAAQKFLEQAAVENLPTFVVELSKVLANPANSQVARVAAGLQIKNPLTSRDPDVKAQYQQRWLAIDASARGEIKTYVLRTLGTESYRPSSASQCVAGIACAEITVNQWPQLIPQLVANVTDPNSTERMKESTLEAIGYICQDIDPEQLQHKSNEILTAIIQGMRKEEPSNNVRLAATNALLNSLEFTKANFDKESERHYIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITVEAMKNEIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPESCQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPSSYCLSSSFEVIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQVESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGAEFLKYMEAFKPFLTIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEMMQFLLENLGNENVHRSVKPQILSVFGDVALAIGGEFKKYLDVVLNTLQQASQAQVDKSDYDMVDYLNELREGCIEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDSVVACGAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKTKTLATWATKELRKLKNQA q6dcp4_xenla Nup98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MFNKTFGSPFGTGNGAFGATSTFGQTTGFGTTPAAAFGSAGFGTNTTTGGLFGNTQTKPGSLFGANTFNQPATSSASSGFGFGASTGTTNSLFGSTNTGSSLFSTPSNAFAQTKPASFGNFGTSTSTGGLFGNTNTANPFSGTSASLFGASTFSSAPTGTTIKFNPQSGTDTMAKGGVTTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNPVGAPAGTGLFGTSAATSSAPTGIFGSTAANSFSFAGNKTTFGAAGTGAFAANTGGLFGQPTNPPAASLFNKPFGNAATTQSTGFSFGNTSTLGQAQTSTMGLFGANQATQAGGLFGATTNTNAAGAFGAGSSLFGQPNPAPFGGGSTLFGNKPAGFGTTTTTAPAFGTTTGGLFGNKATLTLGTNTNTSNFGFGANAAGTSLFGNKPATGTIGPSLGTSFGTALNPGQTSLFGGNQPKLTGTLGTGAFGGTGFNSTSTGLGFGAPQPAMATLTDPNASAAQQMFLQQQYNALRYSPFPDSPLFRNPISDPKKKEELLKPTNPAAQKAVLTPTHYKLTPRPAARVRPKALQNSGAAKSQLFDALDDDEPVLSSGVFMPKKSIKKLDLKYLKNSSSLFAQESKDVNEVVPPTPETQDRSVENHHADEKKKK if23a_xenla Insulin-like growth factor 2 mRNA-binding protein 3-A KH domain-containing transcription factor B3-A Vg1 localization element binding protein A Vg1 RNA-binding protein A RNA-binding protein Vera-A VLE-binding protein A VgLE-binding and ER association protein A 69 kDa RNA-binding protein A Trans-acting factor B3-A VICKZ family member 3-A IGF-II mRNA-binding protein 3-A igf2bp3-a vera-a vickz3-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MNKLYIGNLSENVSPTDLESLFKESKIPFTGQFLVKSGYAFVDCPDETWAMKAIDTLSGKVELHGKVIEVEHSVPKRQRSRKLQIRNIPPHLQWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYANKEHARQGLEKLNGYQLENYSLKVTYIPDEMATPQAPSQQLQQQPQQQHPQGRRGFGQRGPARQGSPGAAARPKPQTEVPLRMLVPTQFVGAIIGKEGATIRNITKQTQSKIDIHRKENAGAAEKPITIHSTPEGCSAACKIIMEIMQKEAQDTKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQDLTLYNPERTITVKGSIEPCAKAEEEIMKKIRESYENDIAAMNLQAHLIPGLNLNALGLFPSSSSGMPPPSVGVPSPTSSTSYPPFGQQPESETVHLFIPALAVGAIIGKQGQHIKQLSRFAGASIKIAPAEGPDAKLRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIKVPSYAAGRVIGKGGKTVNELQNLTSAEVVVPRDQTPDENDEVVVKITGHFYASQLAQRKIQEILAQVRRQQQQQQKTVQSGQPQPRRK q8ax85_xenla khsrp.S Khsrp khsrp protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSDYPGAQSGNRKDAFADAVQRARQIAAKIGGDVTGGVNNTPEFNFGGQKRQLEDGDMFGFPSPDQPECKKLATQPESMPPQLAPVHPPRSSSMTEEYRVPDGMVGLIIGRGGEQINKIQQESGCKVQISPDSGGMPERIVSLTGNPDAVQKAKMLLDDIVLRGRGGPPSQFHDSSNGQNGSLQEIMIPAGKAGLIIGKGGETIKQLQERAGVKMILIQDGSQNTNMDKPLRIVGEPFKVQQACEMVMDLLKERDQPNFDRNEYGTRGGGGGGGGGGGIDVPVPRHSVGVVIGRSGDMIKKIQNDAGVRIQFKQDDGTGPDKIAHIMGPPDRCEHAASIISDLLQSLRTGPPGPPGPGMPPGGRGRGRGQGPWGPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQPPPNGDPNFKMFIIRGNPQQIDHAKQLIEEKIEGPLCPIGPGPPGPGPAGPMGPYNPGPYQPGPPGGPPHQGPPPPAAHQYPPQGWGSTYPQWGQPPAAHDPTKPPAPTDPSAAWAAYYSHYYQQPSAPVPGQPPAVPAPPPQGEPPQQPPSANQPDYTKAWEEYYKKMGQQTPQPAGQPDYTKAWEEYYKKQAATPGAPAAAVAAAAATAAPPAAQPDYSAAWAEYYRQQAAYYGQAPGAPPTQPPSAQPGPQAQ l14aa_xenla Protein LSM14 homolog A-A lsm14a-a lsm14a rap55a-a RNA-associated protein 55A-A protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIPPRDEIFEYIIFRGSDIKDLTVCEPPKPQCSLPQDPAIVQSSLGSSSASSFQSVSSYGPFGRMPAYSQFNTGPLVGPQFGAVGVGSSLTSFGAETTSSTSLPPSSAVGTSFTQEARTLKTQSSQGQSSSPLDSLRKSPNIEQAVQTAAAPHAPSTATVGRRSPVLSRPVPSSIQKTAESPEQRKGELHKMQRPDIDQLKNDKNDPSKRQPVLSALQPRRGRGGNRGGRGRFGVRRDGPMKFEKDFDFESANAQFNKEEIDREFHNKLKIKDDKPEKPVNGEDKTDSVVDTQNSEGNAEEEEVLAGGVCYYDKTKSFFDNISCDDNRDRRQTWAEERRINVETFGLPLRSNRGRGGFRGRGGGMGFRGGRGRGGERRGAPGGGGFGPARGFRGGFRGGRGGREFADYEYRKDNKVAA ilf3a_xenla Interleukin enhancer-binding factor 3-A ilf3-a ilf3 ubp3 Double-stranded RNA-binding protein 4F.1 CCAAT box transcription factor subunit protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRPMRIFLNDDRHVMAKHSVVYPTQEELEAVQNMVSHTERALKAVSDWIDQQEKDCSGEQEQPMAEETETTEEGKDSEMKTGENPTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCRDKPTISLLKRVADNLVLQFETVSEDKYEVIQNIREALIVVKSTKEPPLTLNIRLTSPLVREEMEKLSAGETLTVSDPPDVLDRHKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWEPLRGWPLELLCEKAIGTANRPMGAGEALRRVLECLSSGILMPDGSGLYDPCEKDASDALEHLERQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKTKVETPVIDYTVQIPPSTTYAMPPLKRPIEEDGDDKSPSKKKKKIQKKDEKSEPPQVMNALMRLNQLKPGLQYKLISQTGPVHAPVFTMSVEVDDKTFEASGPSKKTAKLHVAVKVLQDMGLPTGMEEKEEGTDESEQKPVVQTPAQPDDSAEVDSAALDQAESAKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGVKFQGSGSNKKVAKAYAALSALEKLFPDYTMYTEAPKKKRPPMMPRGGPKFAGKHNQGFGMMYSEVPPPQAMRGRGRGGMNRGRGRGRGGFGGGYMNSGGYGGGYGGNYNYQTSATAGYSQFYSNGGASGNAGGGGAGTGGYSSYYQGDSYSAPTPPKPFVNKKPPPPQQQQQQQPPPQHASNPPKPSYNQGYQGHQGGQQQQQQQQQQQTYNQNQYSNYGPPQKQKGGYNQGAQGAGSGGSYNYSNSYTGGTAPLGTAVERVQEGEAAALTQRQALVTTQAHTPAMVEPAVLRRTKVTRSLITIKVPPDRTTVALQIITSSLREELGVTAGIQNTT tacc3_xenla Transforming acidic coiled-coil-containing protein 3 Cytoplasmic polyadenylation element-binding protein-associated factor Maskin tacc3 maskin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSLQIINDENAGNDITAEKFDLLLNPQTTGRPSILRPSQKDNLPPKPALKSTKVTFQTPMRDPQTLRIMTPSVANKPENVFLLEDCTQALEQLHLSLPSSCGPNPVEINVSVSNNQPDSEELPVRTTGAYSIDFDNFDDINPFKSKTQMLNSPIKADLPSLTENIETTTPVVPADEASKQMVSLNLSAANLDSPVTVQFSSESGSIGVSEKTALDDTLPLSESGIKDLQGLNASSNNIEEPVPLDKDICSNNEKDDATMADSTCEGTSDAHTSSNNIEEPVTLDKDICSSNEKDNAAVADSTCEGTSDAQSPLPIPKSSYSFDPDQFDMMNPFKTGGSKLQNSPAGKKQTPPSADLNTAKTEPVKLEFNFGDGDVSERKPPPKKLGKRPLLKTAAKKPSPKPEIASEKQEQQTAKPSEDEAIVPKASYKFDWEKFDDPNFNPFGCGGSKISSSPKGQKIANEQPSACTQGSKPEAECTASDMAPAENADEKDHGEIEPSQDSGAAEDRSQAEDQSVALSKVEVPHEQTTDCSPVENETQPEVSLINEEPSQKEVEHTSSDMTPPEINGTDSEFKLATEADFLLAADMDFKPASEIFSEGFRQPVEIDYLENFGTNSFKESVLRKQSLYLKFDPLLRESPKKSAAGINLLPSVPLKCSSDLFGAIPEANFPLIPSIENEEKPKGLDLLGTFTVADTALLIVDAPSSVAVPNPFLSTSDAIVEMLKYSQKDMDAAIEAVRLEVQEKDLEVLEWKTKHEKLYLEYVEMGKIIAEFEGTITQILEDSQRQKETAKLELNKVLQEKQQVQVDLNSMETSFSELFKRLEKQKEALEGYRKNEEALKKCVEDYLVRIKKEEQRYQALKAHAEEKLNRANEEIAHVRSKAKSEATALQATLRKEQMKIQSLERSLEQKSKENDELTKICDDFILKMEKI kpnb1-rae1-1 201024 N/A 201028 unspecified role unspecified role prey prey xenla-oocyte Xenopus laevis oocyte Oocyte N/A 201029 unspecified role unspecified role bait bait n-terminal s-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-teminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false By RanQ69L-GTP. rae1-kpnb1-1 201025 N/A 201028 unspecified role unspecified role bait bait N/A 201029 unspecified role unspecified role prey prey physical association physical association false false false By RanQ69L-GTP. kpnb1-rae1-2 201026 N/A 201029 unspecified role unspecified role bait bait n-terminal s-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 201028 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false By GST-Nup98. rae1-kpnb1-2 201027 N/A 201030 unspecified role unspecified role prey prey N/A 201031 unspecified role unspecified role prey prey N/A 201032 unspecified role unspecified role prey prey N/A 201033 unspecified role unspecified role prey prey N/A 201034 unspecified role unspecified role prey prey N/A 201029 unspecified role unspecified role prey prey N/A 201028 unspecified role unspecified role bait bait N/A 201035 unspecified role unspecified role prey prey association association false false false RNAse treatment of this complex abrogates mitotic spindle organizing activity. Other uncharacterized subunits may exist.