IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ guo-2003-1 Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Guo DF., Chenier I., Tardif V., Orlov SN., Inagami T. Figures 1A and 1B. guod@magellan.umontreal.ca 10.5 day mouse embryo cDNA library used. Biochem. Biophys. Res. Commun. (0006-291X) 2003 guo-2003-2 Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Guo DF., Chenier I., Tardif V., Orlov SN., Inagami T. Figures 1A and 1B. guod@magellan.umontreal.ca 10.5 day mouse embryo cDNA library used. Biochem. Biophys. Res. Commun. (0006-291X) 2003 guo-2003-3 Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Guo DF., Chenier I., Tardif V., Orlov SN., Inagami T. transient transfection of constructs Figures 1A and 1B. guod@magellan.umontreal.ca 10.5 day mouse embryo cDNA library used. Biochem. Biophys. Res. Commun. (0006-291X) 2003 guo-2003-4 Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging sequence tag sequence tag identification sequence tag Guo DF., Chenier I., Tardif V., Orlov SN., Inagami T. Cells were co-transfected with Flag epitope-tagged AT-1A and GFP-ARAP1 fusion protein and treated with 100nM Angiotensin II for 1, 15 and 45 mins, localization was studied by immunocytochemical analysis with confocal microscopy. Figures 1A and 1B. guod@magellan.umontreal.ca 10.5 day mouse embryo cDNA library used. Biochem. Biophys. Res. Commun. (0006-291X) 2003 arap1_mouse Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 Arap1 Centd2 Kiaa0782 Centaurin-delta-2 protein protein mouse Mus musculus Mouse MAEGYDAALSVAEWLRALHLEQYTALFEQHGLVWATECQGLSDAGLLDMGMHLPGHRRRILAGLHRAHAPPVPLPRPAPRPVPMKRHIFRSPPVPVTPPEPPPTAGEDEGLPAAPPIPPRRSCLPPACFTPTSTAAPDPVLPPLPAKRHLVEPSVPPVPPRTGPPYPQASLLAKEELLLPSVSPRSQPEPAETPSTLLPAFPQGPLQPPSPPPCPPVIPPKPPRLLPEFDDSDYDDVPEEGPGAPASVMTKEEPLPSRVPRAVRVASLLSEGEELSGDDSEDDDDHAYEGIPNGGWPTSGLNPPLRSLIPDLPLHPMDELPGGPTPITPVIKAGWLDKNPPQGSYIYQKRWVRLDADYLRYFDSNKDAYSKRFVPVACICRVAPIGDQKFEVITNNRTFAFRAESDVERNEWMQALQQAVVEHRARFRLSSASVLGVRGSEQPDRAGSLELRGFKNKLYVAVTGDKVQLYKNLEEFHLGIGITFIDMNVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAAQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTEALIQLFLHLGNGPGNHFWAANVPPSEALEPSSSPGARRYHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGVSCFSGDPAAPTPLALAEQAGQTLQMEFLRNNQSTEVPRLDSVKPLEKHYSVTLPTVSHSGFLYKTASAGKPLQDRRAREEFSRRWCVLSDGVLSYYENERAVTPNGEIRASEIVCLAVSPLDTHGFEHTFEVYTEGERLYLFGLENAELAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPCKAGLSLQQAQEGWFALTGSELRAVFPEGPWEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMAWLGVIQKAAASLGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLDSLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLAWLEASEIEDEEEKISRYRELLVHLPPVNRATVKALISHLYCVQCFSDTNQMNTHNLAIVFGPTLFQTDGQDYKAGKVVEDLINHYVVVFSVDEEELRKQREEVTAIVKMRVAGTASGTQHAGDFICTVYLEEKKVETEQHVKIPASMTAEELTLEILDRRNVSIREKDYWTCFEVNEKEEAERPLHFAEKVLPIVHGLGIDSHLVVKKYQSMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQRPWSGAPETSHRPEKEWPVKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLSVQHDGLVWPSEPSRVSRAVPEVRMGSVSLIPLRGSENEMRRSVAAFTADPLSLLRHV agtra_rat Type-1A angiotensin II receptor Angiotensin II type-1A receptor Agtr1 Agtr1a At1a protein protein rat Rattus norvegicus (Rat) Rat MALNSSAEDGIKRIQDDCPKAGRHSYIFVMIPTLYSIIFVVGIFGNSLVVIVIYFYMKLKTVASVFLLNLALADLCFLLTLPLWAVYTAMEYRWPFGNHLCKIASASVSFNLYASVFLLTCLSIDRYLAIVHPMKSRLRRTMLVAKVTCIIIWLMAGLASLPAVIHRNVYFIENTNITVCAFHYESRNSTLPIGLGLTKNILGFLFPFLIILTSYTLIWKALKKAYEIQKNKPRNDDIFRIIMAIVLFFFFSWVPHQIFTFLDVLIQLGVIHDCKISDIVDTAMPITICIAYFNNCLNPLFYGFLGKKFKKYFLQLLKYIPPKAKSHSSLSTKMSTLSYRPSDNMSSSAKKPASCFEVE q5i0h0_rat Centd2 protein protein rat Rattus norvegicus (Rat) Rat IEDEEEKISRYRELLVHLPPVNRATVKALISHLYCVQCFSDTNQMNTHNLAIVFGPTLFQTDGQDYKAGKVVEDLISHYVVVFSVDEEELRKQREEVTAIVKMRVAGTASGTQHAGDFICTVYLEEKKVETEQHVKIPASMTAEELTLEILDRRNVSIREKDYWTCFEVNEKEEAERPLHFAEKVLPIVHGLGMDSHLVVKKYPSMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPVKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLSVQHDGLVWPSEPSRVSRAVPEVRMGSVSLIPLRGSENEMRRSVAAFTADPLSLLRHV centd2-agtr1 Interaction of AT-1A recpetor and ARAP1, detected by yeast two hybrid. 257023 N/A 257027 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 257028 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false centd2-agtr1-2 Interaction of AT-1A receptor and ARAP1 detected by yeast two hybrid. 257024 N/A 257027 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 257028 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false The truncated fragment of AT-1A (P25095) of 295-318AA did not interact with ARAP1 (Q4LDD4)(shown in Figure 1C). agtr1-cendt2-3 Interaction of rat ARAP1 and AT-1A, detected by co-immunoprecipitation. 257025 N/A 257028 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 257029 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false A truncated mutant of AT-1A (P25095) containing 1-318AA did not interact with ARAP1, indicating that the carboxyl terminal region between residues 319-359 of the receptor is important for the interaction. The presence of Angiotensin II promoted the association beween the AT-1A receptor and rat ARAP1 (Q510H0), with maximum effect after 30-45 mins of exposure to Ang II. cendt2-agtr1-4 ARAP1 is co-localized with the AT-1A receptor in the plasma membrane 45 min after stimulation with Ang II. 257026 N/A 257028 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 257029 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. colocalization colocalization false false false