<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2005-09-02+01:00">
            <names>
                <shortLabel>IntAct</shortLabel>
                <fullName>European Bioinformatics Institute</fullName>
                <alias typeAc="MI:1041" type="synonym">IntAct</alias>
                <alias typeAc="MI:1041" type="synonym">intact</alias>
            </names>
            <bibref>
                <xref>
                    <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
            </bibref>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="22121220" refType="method reference" refTypeAc="MI:0357"/>
            </xref>
            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
                <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
                <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
                <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
                <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
                <attribute name="url">http://www.ebi.ac.uk/</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="207617">
                <names>
                    <shortLabel>mcnally-2003-1</shortLabel>
                    <fullName>Structural analysis of UBL5, a novel ubiquitin-like modifier.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="12824502" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-633398" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="4932">
                        <names>
                            <shortLabel>yeasx</shortLabel>
                            <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
                            <alias typeAc="MI:1041" type="synonym">Baker's yeast</alias>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>2 hybrid</shortLabel>
                        <fullName>two hybrid</fullName>
                        <alias typeAc="MI:0303" type="go synonym">2H</alias>
                        <alias typeAc="MI:0303" type="go synonym">2h</alias>
                        <alias typeAc="MI:0303" type="go synonym">Gal4 transcription regeneration</alias>
                        <alias typeAc="MI:0303" type="go synonym">two-hybrid</alias>
                        <alias typeAc="MI:0303" type="go synonym">yeast two hybrid</alias>
                        <alias typeAc="MI:0303" type="go synonym">2-hybrid</alias>
                        <alias typeAc="MI:0303" type="go synonym">classical two hybrid</alias>
                        <alias typeAc="MI:1041" type="synonym">classical two hybrid</alias>
                        <alias typeAc="MI:1041" type="synonym">2H</alias>
                        <alias typeAc="MI:1041" type="synonym">two-hybrid</alias>
                        <alias typeAc="MI:1041" type="synonym">2-hybrid</alias>
                        <alias typeAc="MI:1041" type="synonym">Gal4 transcription regeneration</alias>
                        <alias typeAc="MI:1041" type="synonym">2h</alias>
                        <alias typeAc="MI:1041" type="synonym">yeast two hybrid</alias>
                        <alias typeAc="MI:1041" type="synonym">Y2H</alias>
                        <alias typeAc="MI:1041" type="synonym">Y-2H</alias>
                        <alias typeAc="MI:1041" type="synonym">2 hybrid</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0018" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-94" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="12634794" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="1946372" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="10967325" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>predetermined</shortLabel>
                        <fullName>predetermined participant</fullName>
                        <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">McNally T., Huang Q., Janis RS., Liu Z., Olejniczak ET., Reilly RM.</attribute>
                    <attribute name="contact-email" nameAc="MI:0634">Edward.olejniczak@abbott.com</attribute>
                    <attribute name="journal" nameAc="MI:0885">Protein Sci. (0961-8368)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2003</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="207618">
                <names>
                    <shortLabel>ubl5_human</shortLabel>
                    <fullName>Ubiquitin-like protein 5</fullName>
                    <alias typeAc="MI:0301" type="gene name">UBL5</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9BZL1" version="SP_40" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000466910" version="SP_147" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:1903955" secondary="positive regulation of protein targeting to mitochondrion" version="SP_147"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001041706.1"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000586895" version="SP_147" refType="transcript"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000468656" version="SP_147" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031386" secondary="protein tag" version="SP_147"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6AHD" version="SP_147"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000626" version="SP_147"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7ABG" version="SP_147"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0000398" secondary="mRNA splicing, via spliceosome" version="SP_147"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" secondary="nucleus" version="SP_147"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000358666" version="SP_147" refType="transcript"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7AAV" version="SP_147"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_077268.1"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-607755" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039732" version="SP_147"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1P0R" version="SP_147"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q2NL89" version="SP_40" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7ABF" version="SP_147"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR029071" version="SP_147"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" secondary="cytoplasm" version="SP_147"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7ABI" version="SP_147"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000590068" version="SP_147" refType="transcript"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000198258" version="SP_147" refType="gene"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000351492" version="SP_147" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0006464" secondary="cellular protein modification process" version="SP_147"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4PYU" version="SP_147"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="9606">
                    <names>
                        <shortLabel>human</shortLabel>
                        <fullName>Homo sapiens</fullName>
                        <alias typeAc="MI:1041" type="synonym">Human</alias>
                    </names>
                </organism>
                <sequence>MIEVVCNDRLGKKVRVKCNTDDTIGDLKKLIAAQTGTRWNKIVLKKWYTIFKDHVSLGDYEIHDGMNLELYYQ</sequence>
            </interactor>
            <interactor id="207619">
                <names>
                    <shortLabel>clk4_human</shortLabel>
                    <fullName>Dual specificity protein kinase CLK4</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">CDC-like kinase 4</alias>
                    <alias typeAc="MI:0301" type="gene name">CLK4</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9HAZ1" version="SP_50" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" secondary="nucleus" version="SP_164"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008271" version="SP_164"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-633400" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005524" secondary="ATP binding" version="SP_164"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0106311" secondary="protein threonine kinase activity" version="SP_164"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011009" version="SP_164"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000316308" version="SP_164" refType="transcript"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0004712" secondary="protein serine/threonine/tyrosine kinase activity" version="SP_164"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000719" version="SP_164"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017441" version="SP_164"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6FYV" version="SP_164"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000316948" version="SP_164" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0004714" secondary="transmembrane receptor protein tyrosine kinase activity" version="SP_164"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0046777" secondary="protein autophosphorylation" version="SP_164"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0106310" secondary="protein serine kinase activity" version="SP_164"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000113240" version="SP_164" refType="gene"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0043484" secondary="regulation of RNA splicing" version="SP_164"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_065717.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0004674" secondary="protein serine/threonine kinase activity" version="SP_164"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="9606">
                    <names>
                        <shortLabel>human</shortLabel>
                        <fullName>Homo sapiens</fullName>
                        <alias typeAc="MI:1041" type="synonym">Human</alias>
                    </names>
                </organism>
                <sequence>MRHSKRTHCPDWDSRESWGHESYRGSHKRKRRSHSSTQENRHCKPHHQFKESDCHYLEARSLNERDYRDRRYVDEYRNDYCEGYVPRHYHRDIESGYRIHCSKSSVRSRRSSPKRKRNRHCSSHQSRSKSHRRKRSRSIEDDEEGHLICQSGDVLRARYEIVDTLGEGAFGKVVECIDHGMDGMHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIVFELLGLSTYDFIKENSFLPFQIDHIRQMAYQICQSINFLHHNKLTHTDLKPENILFVKSDYVVKYNSKMKRDERTLKNTDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMMERILGPIPQHMIQKTRKRKYFHHNQLDWDEHSSAGRYVRRRCKPLKEFMLCHDEEHEKLFDLVRRMLEYDPTQRITLDEALQHPFFDLLKKK</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction id="207620">
                <names>
                    <shortLabel>clk4-ubl5-1</shortLabel>
                    <fullName>Interaction between UBL5 and CLK4 identified by Y2H</fullName>
                </names>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-633407" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>207617</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="207621">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-633408" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>207618</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>bait</shortLabel>
                                    <fullName>bait</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                    <participant id="207622">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-633409" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>207619</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>prey</shortLabel>
                                    <fullName>prey</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="isoform-comment" nameAc="MI:0637">Interaction is between UBL5 and full length CLK4. A C-terminally truncated splice variant does not interact with UBL5</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
