MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint gruenhei-2001-1 Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Gruenheid S., DeVinney R., Bladt F., Goosney D., Gelkop S., Gish GD., Pawson T., Finlay BB. bfinlay@interchange.ubc.ca Nat. Cell Biol. (1465-7392) 2001 gruenheid-2001-1 Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy predetermined predetermined participant predetermined Gruenheid S., DeVinney R., Bladt F., Goosney D., Gelkop S., Gish GD., Pawson T., Finlay BB. bfinlay@interchange.ubc.ca Nat. Cell Biol. (1465-7392) 2001 gruenhei-2001-2 Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. in vitro In vitro experimental interac experimental interaction detection experimental interac predetermined predetermined participant predetermined Gruenheid S., DeVinney R., Bladt F., Goosney D., Gelkop S., Gish GD., Pawson T., Finlay BB. bfinlay@interchange.ubc.ca Nat. Cell Biol. (1465-7392) 2001 gruenhei-2001-3 Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gruenheid S., DeVinney R., Bladt F., Goosney D., Gelkop S., Gish GD., Pawson T., Finlay BB. bfinlay@interchange.ubc.ca Nat. Cell Biol. (1465-7392) 2001 q5wmc9_ecolx Secreted effector protein Tir Translocated intimin receptor Tir tir protein protein ecolx Escherichia coli MPIGNLGNNVNGNHLIPPAPPLPSQTDGAARGGTGHLISSTGALGSRSLFSPLRNSVADSVDSRDILGLPANPSRLAAATSETCFLGGFEVLHDKGPLDTLNQQIGPSAFRVEVQADGTHAAIGEKNGLEVSVTLSPQELQSLQSIDIEGKNRFVFTGGRGGSGHPMVTTASDIAEARTKILAKLAPDNHGGRQPKDVDTRSVGVGSASGIDDGVVSETHTSTTNSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAANAAESATKDQLTQEAFKNPENQKVNIDANGNAIPSGELRDDVVAQIAEQAKEAGEVARQQAVESNAQAQQRYDEQHARRQEELQLSSGIGYGLSSALIVGGGIGAGVTTALHRRNQPAEQTTTTTTHTVVQQQTGGNTPAQGGTDATRAEDASLNRRDSLGSVALTHWSDSSSEVVNPYAEVGGARNSLSAHQPEEHIYDEVAADPGYSVIQHFSGSGPVTGRLIGTPRQGIQSTYALLANSGGLRLGMGGLTSGGESAVSSVNAAPTPGPARFV mint nck1_human Cytoplasmic protein NCK1 NCK1 NCK NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha protein protein human Homo sapiens Human MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS nck1-tir 174731 q5wmc9_ecolx tir 174735 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylated phosphorylated residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 nck1_human NCK1 174736 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 direct interaction direct interaction false false false F2D 313 nanomolar mint nck1-tir-1 174732 q5wmc9_ecolx tir 174735 experimental particp experimental participant identification experimental particp unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed phosphorylated phosphorylated residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 nck1_human NCK1 174736 experimental particp experimental participant identification experimental particp unspecified role unspecified role unspecified role unspecified role physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 colocalization colocalization false false false F1 mint tir-nck1 174733 q5wmc9_ecolx tir 174735 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false phosphorylated phosphorylated residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 nck1_human NCK1 174736 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 association association false false false F2C experimental=gel overlay mint tir-nck1-1 174734 q5wmc9_ecolx tir 174735 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified phosphorylated phosphorylated residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 nck1_human NCK1 174736 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association false false false F2B Phosphorylation of Tir is required for the interaction mint