IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2004-1 SNF-6 is an acetylcholine transporter interacting with the dystrophin complex in Caenorhabditis elegans. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim H., Rogers MJ., Richmond JE., McIntire SL. Nature (0028-0836) 2004 snf6_caeel Sodium-dependent acetylcholine transporter Sodium:neurotransmitter symporter family protein 6 snf-6 M01G5.5 protein protein caeel Caenorhabditis elegans MSVSSNDPEQRNGRGMASGNNVDMSLYPPFIKQLDAKLPDYTREGDIEYPFEEITGVGDENRIRGNWSNKSDYLLAVIGFTAGVGSFWKFPFLVFQHGGAAFLVPYLCMLCLASLPMFFMEMVLGQFSSSAAISVWKVVPLFKGIGFAQVTISGFFAVFFNIISAWTLFYLINSFSFSIPWSNCANSWSGENCTLGTRIQCKEMNGTLLVNGSCIVEHASSNETTVIPLHDLGSIPSLKYFQNDVLMLSKGVDDFGTLNWYLGLCVLACWIAVFLCLFQGVKSSGKVVYVAVIVPFIILTVLLTRLLTLDGSLAAVFYFLTPKWEILMDLHVWGEAAVQAFYSVSCCSGGLFTIASYSRFHNNIYKDIWLVLIVDVIVSLVGCLLTFSAIGFTCYEFAISLDKFHIRDGFHLVFVFLAEALAGVSVAPLYAGLFFIMILLVVHATQMFVVETIVSSICDEYPERLRRNRRHVLTTVCALFILLSIPFCLSSGLFWMELLTQFVLTWPLVVIAFLECMAINWVYGVDNMLDNAKWIVGYWPPCYIFWKILFKFICPMVYLAILCFLWLDWNSIQYESYQFPYWSILTAWCIASFPLILIPIVGIWQFCIAKGTITQKWWRVLYPDDAWGPAMAIHRAEKFPLQIPEARRLLLPPEVEIASSRGVLQEEMPMSYDYNTSSAADVRSNRSTGHGATDVRSVAATNNTIPKFERETAI snt1_caeel Syntrophin-1 F30A10.8 stn-1 protein protein caeel Caenorhabditis elegans MAAVRSGLVDIFVQGQWHRVLATLDPTAITLQTMEQNEAEAEKRTVRVVKYDGNGLGISIKGGRDNNMPIVISKIFKGMAADQAGELFLDDVIISVNGENLLDASHEEAVRALKRAGRVVDLQVQYRREDMMHRENIVENVEWDDDIRERVRTIGLKLAYVARAGIDADAEGRILEMRSPSGRYSLAMRCSSSEEADGWFEALHACTTCLLTQALAQVNIMLGNNPQVRHMGWIAEQVSENGISMWKPKFMTLTNSEILFYEAVPQLKAEWAEPRLVRPLVATRVVQTSSRTAPVIKGLTDVISFRMRTGTQQGVRTHTIRVETHAELARWVRAVVIGGYEACLSTSQVSAPCLWRGESCELIVNLDNGISLLSSTGEVLWQHSFETIRATGDDGGRFLWVDFGPPHGEQELDLLNSAKPVVFILHSFLATKVYRLGLYA snf6-stn1-1 stn1 interaction with full length snf6 and snf6 lacking three c-terminal amino acids. 145935 N/A 145936 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 145937 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false The snf-6 and stn-1 interaction was detected using an anti-FLAG antibody. Reciprocal interaction was observed with a combination of stn-1 and snf-6 but not with stn-1 and the snf-6 lacking three c-terminal amino acids (712-714), suggesting that these are important for the binding to stn-1. snf6-stn1-2 stn1 interaction with full length snf6 and snf6 lacking three c-terminal amino acids. 145935 N/A 145937 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 145936 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false The snf-6 and stn-1 interaction was detected using an anti-HA antibody. Reciprocal interaction was observed with a combination of stn-1 and snf-6 but not with stn-1 and the snf-6 lacking three c-terminal amino acids (712-714), suggesting that these are important for the binding to stn-1.