IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhong-2003-1 A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhong J., Zhang H., Stanyon CA., Tromp G., Finley RL. A modification of the pooling method and array method was used in which a small pool of AD constructs (96) is mated with an array of BD constructs and then the BD which shows interaction is mated with an array of the AD constructs to identify the interacting partners. Genome Res. (1088-9051) 2003 q9gtq8_drome CycD CG9096 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADIMDLLCSEIIVYDSDPSLYRLNKRQQQMSSPAIIRDQSSICSSLGDAESDSHPDAQNNAVVCNMKELVYIYASVDSAAKNPEQLEPPPPPPPPPPPPPTATQSIQSYPRYISQEPPTSHCQRLDERLTTTADPPATDNVNTAIGDPTLYSDRCLENFLKVEEKHHKIPDTYFSIQKDITPPMRKIVAEWMMEVCAEENCQEEVVLLALNYMDRFLSSKSVRKTQLQILAAACLLLASKLREPSCRALSVDLLVVYTDNSIYKDDLIKWELYVLSRLGWDLSSVTPLDFLELLMMRLPIGSKNFPDINIGKVRGHAQAFISLAAKEHKFAKFSASTIAASSIAASMNGLKWHLRSGHNLHFLLSLMTDLTSVEQAQVRDCMLHMEDIFKEHSRNLEPFLVNIDPKEMSTLYYKRRFQIHQSQHLSQISIRPLPLPKAPAECVEQHQQQHNFGSAAPHRTHTCKMQAQAQAQNEIQDVTF q8ms31_drome mute CG15610 CG33455 CG33456 Dmel\CG34415 Mute CG34415 Dmel_CG34415 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFCSNTNSKTAAQPKRSRSDDEAAKLRRANRQEEMLRQMLQPDTTEERNRKDAIFAMNFYEKVEEALLSANRLDDCKKFNSILQNFDPRQEKVSSLYLKVEEILLPTHPELAEVFLNFLLPAEAAEMGKFFEHFMICNATNFINKLNIYFSKQPAQIRKIYACLNELAEMPNVSMKKVENKIMPLLKGNQFLIDWFVQQFPQGKPPKRILSPVETINLHDVQNNTTGEYTETIQEFTDVPTPSKPGCHLKYINGRIFYGAKILLPAKLSFMAASIYNEQSHDVPPVSSDSINCAHGIRAQGEKLLSMASNTDSDDGADRSEEDDASRALIIDTTSDEQNSCSSIEMCDETGLRAHAIRLNPGLYTNSSFNNAATSTPNLGNSHQSHHPHAKKTAINFGEISPRKQSAFNNSGLSPLSAAPNSSPQVDKRKSPTKKIRSPLNQTQSRQRANNQPMVVIHESQPSSSPAIECAKRLRTLIDQESVRDAADTKAGIRIIAQAKQQPPNQCSSEAITKVEVVSLDESAENMPFCTNLKMDTDQSEEELEPLQLSAGNTPQHVITTQFDSDEDCKLDVVATLANCTTTEDRSESAAALPATTSHSESGSSHSAPWTREEDKVILIEMKIGARDREQLIRRMRAKLKNRSIEELRSRHQFLMDFLSKLQGK q24159_drome CycJ CG10308 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly FNFDTMEQKVAAEQNIFVVDRKLKKTCPQADVERLAKTHWLTDYARDIFLTMREQELSRRPLFYLSPQLNERRRMLQLLKLATSAHKLSRLALHLAVYYMDRFVDYYKIRPDKLLLVAITCLHIAAQIENTDAFIPRYSEMNRLVKNAYTAFEYKAVERKILCFLNFELIRPTTASFVELFACSFLTRSDFKNYIEMLDEYERIHHTQPYQRYISFEEMLSILAQLLLRMADYTLYISRFANDLPSLLAAACIAAVRQVSGVRRWSEYLVGLTSYTEAHVEPYMNVLTDYHYYHVIQADYGSPSVQTNQSLASPDSGFEESFTENTNLVVSDEVVTVETYNIITVQLQDPSPHSSTFLPKEQTNLKRSRFEDDTENQHPLKHAKVESVAKD cdk2_drome Cyclin-dependent kinase 2 Cdk2 Cell division control protein 2 cognate cdc2c CG10498 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV cg15610-cycd-1 Interaction detected by Y2H. 157310 N/A 157311 unspecified role unspecified role prey prey N/A 157312 unspecified role unspecified role bait bait physical association physical association false false false Interaction scored as growth on LEU and X-Gal activity. cdc2c-cyc5-1 Interaction detected by Y2H. 157310 N/A 157313 unspecified role unspecified role prey prey N/A 157314 unspecified role unspecified role bait bait physical association physical association false false false Interaction scored as growth on LEU and X-Gal activity.