IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ estevez-2003-2 The roles of intersubunit interactions in exosome stability. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Estevez AM., Lehner B., Sanderson CM., Ruppert T., Clayton C. aestevez@ipb.csic.es J. Biol. Chem. (0021-9258) 2003 estevez-2003-1 The roles of intersubunit interactions in exosome stability. 9tryp Trypanosoma brucei tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Estevez AM., Lehner B., Sanderson CM., Ruppert T., Clayton C. aestevez@ipb.csic.es J. Biol. Chem. (0021-9258) 2003 q95z11_9tryp RRP45 protein protein 9tryp Trypanosoma brucei MQYQRAALVIASPALTQREVEFTKSAWLAGLRPDQRTAQQMREVSVEFPLLSRDVVVVRCGGSVVTAAIATALVEPTPQRPKHGLLHFSVRRLHTEKDSPAADGSTQKNLVAFLERLVRTGGVIDTAGLCVLPGQKVWSLTVNVTIMNDEGNCNDAAVWAVIALLMHHRRPELTVRGSSVIVHPPHEREPVPLSVQHTPLPFTFAITMAPTEREKALNHQRALSATGSSSGPQPSSGVGNSIDTASAFAIDGTSPQPLGVVVDPTTAESLAAASSIVVAINAEGQVCTVVKAEGCSIHLKDLNACIEAAGTLAPRVLDLIKVAMEAHDERRKTAMKGQFAWAQKRSGVGKVEQTCVKKIKTET q95z13_9tryp RRP41A protein protein 9tryp Trypanosoma brucei MPVIKEYINPAGLRLDGRRQNEPRRLALEFGKISGCDGCCTVVSGLSHVCATVYGPREVSNRLDGKYNEVTITCDVVVAAFAGERRREQQRYSRLSEDISASVLDVARSVVLLSQYPNSQIHICIEVLKQDGSDKAACINAACLALIDASIAMRDVVYTQTVGLIEGFDVVDLTTEEIHSQCPSICITVQGHDPTNIVWMESNSRLAPEVITRLVKVAQKASTDVFETALRGPLEEHAATVLRLQANLGNIE q95z09_9tryp RRP41B protein protein 9tryp Trypanosoma brucei MATISRRDGRTAREMRGKELRTSDLSQFDGSAWYSQGLTAVTVAINGPTAARQENYRRCTTCIHVNRAARIPQEGGTGRLIMMERRERERCEDGELKQFLTSLVEAIVCLDRFPRCVLEAHVTVLMDDGALFAVASNAIMCALLDAGVPCRSTIAAVSLIMCNPEASGNEGEPEIFLDPTAVEESTDEVRSYASATFVLTNTGGEAVLASRVQARPRSSAYSRPITPKDLSRMFALAAKAAETLFVFFRNCSMALE q8mpg3_9tryp Ribosomal RNA-processing protein 43 EAP2 protein protein 9tryp Trypanosoma brucei MSLPPATGAVELASFQQHVHQFLVQNKRLDGRPLDALREPHILRDSKGGDGLLASTLYTDNTGMCLNCTVSGVFGPPPSDQPDEGRLTVDVAAPFFQSHSTDQVAAALREVARFVRSTIISCMDLTELNIINGEACWVLQVELVILNADGGLRAAALHAVVAAIHNMLLPRSRLPNGDEVESRRLRFHTIPVAVTIGVYKLGASGVRFLMDTNAAEESVVDGLLTVVLSDENGIITFVQSGSYSISSLLLTGAVNEFALRSAALRAAILKPSVAAAKINGSKNSVTDPHPLKEG q8iew0_9tryp EAP4 protein protein 9tryp Trypanosoma brucei MERSHGRRSPDEVRYPHIVLNVLGQSYSSACIEVGSTRVVCAVHHPQQLIDEYRGSRGRVACTVRRSSRAQKHELAFRADVTPEKDLALALEGVVEQAVVLEKIPQLLVEVVVEILAEDGGLWDAIATGVCAALASGGFEMYDTFSACGAALLPDGSIVTDPSATEWNTAKATAVTCAMLNSGNMCFTAQRGSCEPTTALDLVTAALKGTLARKNVISSQLCEQFAF q4gyw4_tryb2 TbEAP1 TB927.1.2580 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MQFGPVVSEAEIRTVQDGIANGVRSDGRSLTQRRPFTVVTNRGSTTHFEGSSVEVHCDGTTVVAAATPSVVELDIDRGQAPRGSLFVNIDAVPAVVEHYAQSVRNNSARYRQAFLSFLATAIRQTFGAQGVTAEERQQQLGIAEAEIIADEGDDEQREDAFGGRGAAEEKPVEETAEQRVDCCGFPGEQLYIGGGYAFRIDVDAHVLQAHGGSLASIVALAVHAALKTVRLPSVTLHEASAGFSLEVNYSKPYATAVNWSRLPVLSVVHVSPTRHYVVDPTRLEELALPQQLRVAVNSFGQVFHMRFQQLPSRRGNAMRLIRSPREEAGKDNSNDNSERDVDGDDVDDSSRRGFDAGVKGTVGGMNMPDMAAVLHAAVHISQAVIAECDEALAGSSG q95z17_9tryp CSL4 protein protein 9tryp Trypanosoma brucei MAVIVKTGKCVSPGDVLYATDIYDAIGTIVVAASDTGAVSAGNHADDAIIAGTGCYSRVVSKPDGAVSSQIITTLLGIVQWSGNVVSVRQPTKRARDATTIEVVSLNALKDAGTCGDSATPRLSTPGEATSSVSETPASTAVSTAPKSLWISTSVFGPRMGDNVHLRVVRVSRSFAYGEIIAVNGTWCSNSGSNGGGGGALGGFRGVIRMEDIRPFKPRKDQLTPPPPADAFQDGDVVVATVLSQSDVRQYQLSTVAEHCGVVEAFTVLNSNGRERRVRLRHIPECRDIMKCPLSGNLHRRWCPLIRLV q95z14_9tryp RRP40 protein protein 9tryp Trypanosoma brucei MSETNPVVRGAVGVHELAPLCSHVCLPGESVLLLEPTAVVTLGSGLHPLQQDGSDDRKESFEASGEEGSTVVVSEFCGPVLRTAGPNHTQLYKLNGPAARRYMYAARDPVVAIVMKKNASYYSCYTGAASLAVLDALGFDGATKTNKPRLQEGDVVYAFVKGNADASSLDTKFDDGRVVVSSSDEVELSCMAAEVGLVARDWTSAEAVFGPLVGGTVVRVALPYARSLIDGPASRLLALLGDRVPYDVCVGVNGLVWVRGHPSAADPTAAARRTVAVAACVVEAQDAATLEEMEAHVRGYFPKE q95z16_9tryp RRP4 protein protein 9tryp Trypanosoma brucei MSVPTCITGDTICGSAGPSKLDYVEDHVFLRGYNTYEGNNPNGAAAAQEGGGEIIASINGMVEVTDRVVSVKGMSSRYVAEVGDIVVGRVKEVCGNRWRVDVGAFQDAVMPLANVTEPGGILRRRGRSDELTMRNIFDEGELVVAEVQRVSQDGLISLHTRSGEKYGKLSDFGLLVLISPALVKRVKHHFMCLEFIHVNLVLGTNGAIWVSPATATTATESAKSAYDRFDADIRRAVTRVANCIRVMGNARFPVFDKSIEAAVKASIDFVLGPFEILYPGNQNLITTTVLDTINTRKRTIY q95z10_9tryp RRP6 protein protein 9tryp Trypanosoma brucei MSGSLPQTKDLVNGAFTLVKEYTKLTADLAEEDYDYHMAFPAFRHQITRHSEAVVSLMDKFCQLLPAKRRVTLSSTGGKLTDPQRAAVMEAADSLLENVDGLLDELKGRRLSAKDQLSVKFGSELQGVVPSSSYSVFNAAGVSVLRPQLTFEHPVDNSPTPFRPVYYDEKGVRHVGEPGVHPFAERIKAVSVPSEQLLLKTETPYLSLVTCPLTFVDTVEDLEALVAVLLNETEIAVDLEHHDFYSYQGFTCLMQISTRTQDFIVDCLKVRANMYLMAPVFLQPNIVKVFHGAREDVRWLQKDFGLYIVNLFDTSIALQNLHMPHSLAFAVDHFCQVKLNKKYQTADWRVRPIPAEMVSYAQQDTHFLLYVYDRLKQLLLNCEARASVGNMLLHVFQESRLLSLERYEKPHLDPDVTYKQALGRSLGGLSSPQLQVAREIFNWRDMAAREADDSPSAVMHISCVLSIATKLPTSANEVLKCCSPVSVAVRTNVMKLLQIVKDAIGSADSLKDGANGDVKPKPLPNVGVALRYMGVHRSMTGTLPSIEQRADPAFTVEDAHVVVRTEPSAWFETMRNVAAILRRKPYYPIALPGHDVVTRMSAVKRAREVEEMKVKGEGEEEEKRVHVDAEEPTVERENSSERGEGMAGPCLQEGLEVKDEVEEIDVPTQKPLELKEGAISLHQQYGTGSANRKKARLGKKNKL rrp45-rrp41a-1 Identification of an interaction between RRP45 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role bait bait N/A 134451 unspecified role unspecified role prey prey physical association physical association false false false rrp45-rrp41a-2 Identification of an interaction between RRP45 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134451 unspecified role unspecified role bait bait N/A 134450 unspecified role unspecified role prey prey physical association physical association false false false rrp-45-rrp41b-1 Identification of an interaction between RRP45 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false rrp45-rrp41b-2 Identification of an interaction between RRP45 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role bait bait physical association physical association false false false eap2-41b-1 Identification of an interaction between EAP2 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134452 unspecified role unspecified role bait bait N/A 134453 unspecified role unspecified role prey prey physical association physical association false false false eap2-rrp41b-2 Identification of an interaction between EAP2 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134453 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false rrp41b-eap4-1 Identification of an interaction between EAP4 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role bait bait physical association physical association false false false rrp41b-eap4-2 Identification of an interaction between EAP4 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false eap1-eap4-1 Identification of an interaction between EAP1 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role prey prey N/A 134455 unspecified role unspecified role bait bait physical association physical association false false false eap1-eap4-2 Identification of an interaction between EAP1 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role bait bait N/A 134455 unspecified role unspecified role prey prey physical association physical association false false false csl4-rrp41a-1 Identification of an interaction between CSL4 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134451 unspecified role unspecified role bait bait N/A 134456 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey role arbitarily assigned since not clear in paper csl4-eap4-1 Identification of an interaction between CSL4 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134456 unspecified role unspecified role bait bait N/A 134454 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp40-rrp4-1 Identification of an interaction between RRP40 and RRP4 of Trypanosoma brucei by Y2H 134448 N/A 134457 unspecified role unspecified role bait bait N/A 134458 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp40-csl4-1 Identification of an interaction between RRP40 and CSL4 of Trypanosoma brucei by Y2H 134448 N/A 134457 unspecified role unspecified role bait bait N/A 134456 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp4-csl4 Identification of the components of the Trypanosoma brucei exosome by TAP 134449 N/A 134458 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 134459 unspecified role unspecified role prey prey N/A 134456 unspecified role unspecified role prey prey N/A 134457 unspecified role unspecified role prey prey N/A 134455 unspecified role unspecified role prey prey N/A 134450 unspecified role unspecified role prey prey N/A 134451 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role prey prey N/A 134454 unspecified role unspecified role prey prey N/A 134453 unspecified role unspecified role prey prey association association false false false An eleventh protein EAP3 (EMBL - AC091553) also seen but protein product not yet in UniProt