IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rascle-2003-2 L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rascle A., Stowers RS., Garza D., Lepesant JA., Hogness DS. anne.rascle@dnax.org Third instar larval library was used. Mech. Dev. (0925-4773) 2003 rascle-2003-1 L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rascle A., Stowers RS., Garza D., Lepesant JA., Hogness DS. anne.rascle@dnax.org Third instar larval library was used. Mech. Dev. (0925-4773) 2003 rascle-2003-3 L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom monoclonal western monoclonal antibody western blot monoclonal western Rascle A., Stowers RS., Garza D., Lepesant JA., Hogness DS. anne.rascle@dnax.org Third instar larval library was used. Mech. Dev. (0925-4773) 2003 rascle-2003-4 L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins. drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Rascle A., Stowers RS., Garza D., Lepesant JA., Hogness DS. Adult flies was used. anne.rascle@dnax.org Third instar larval library was used. Mech. Dev. (0925-4773) 2003 q8wti6_drome Pif2 PIF-2 BcDNA:AT26658 Dmel\CG31483 NEST:bs18a02 NEST:bs19e11 NEST:bs20e01 NEST:bs25a03 NEST:bs35g11 bs20e01.y1 pif2 Dmel_CG31483 CG31483 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCSPCCGSCCGPCCSPCCSPCCPPCCNDCCGSCCSPCCGPCCSPCCGPCCSPCCSPCCTPCCTPCCTPCCKCCTPCCVPCCTPCCTPCCTPCCTPCCSPCCGPCCSPCCSPCGGSKCK q7km03_drome Eip63E L63 Dmel\CG10579 EIP63E PFTAIRE Pftaire cdc2-63E Dmel_CG10579 CG10579 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAELISQPTKINNNNFGAGVTMREKKGGALQKLKKRLSHSFGRLTISREDGDESTHHHHHHHHHRGGHGGHHNHGNKVPYNGYNSEEYLDRLEPNGNIPADKTNCRYGAQESAKNDNNKPQDWRSTDSTDHHDRVQRQLSVSSDSKLLDEDIREEMKYHSHVVMRPKKPPRPKSEVFLNKQETHPRRKRFSAFGGDSPFGKQEAYVKLEPLGEGSYATVYKGFSKLTYQRVALKEIRLQEEEGAPFTAIREASLLKELKHSNIVTLHDIVHTRETLTFVFEYVNTDLSQYMEKHPGGLDHRNVRLFLFQLLRGLSYCHKRRVLHRDVKPQNLLISDCGELKLADFGLARAKSVPSHTYSHEVVTLWYRPPDVLLGSTEYSTSLDMWGVGCIFVEMVTGMPTFPGIRDTYDQLDKIFKLLGTPTEDTWPGVTHFPGYKPHKLGFYRPRKLGHNFPRLYDIIEGETIANGFLQLNPEQRLGADDALQHPYFAQLPKKLYELPDETSIFTVEGVQLYTEPNRQNK q8wti7_drome Pif1B PIF-1 CG33720 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MYMQHERHSDSDPNSRHQQTEASHMRDHWKPSKSQQSQQSDRVDPHDKPSQTAVSFLQKHHTPKNPESKANPDDGASCSCTAKRSKSADPPAVKAHTCACNTDPSPCGLGHQEKSSSRKPCSQKTSVTEPEKASSFKSAITACTKELLDPCTCGAHPPYWAGQSNLVTGPSYSRAMDPFSSTLAPLHRPGASKGSFGRSKSLASLPSKSNPPTQSGVTWLSALKDPESTCCSSKEDERPACTCNATQTNQDPSMSSLPQSHPNYRSMTSKSSNFRAPNPRNQPSFGPGDETSATTRSQSIVGQVTCLCASEHQPTKAFVSSLADRECPNEIQIAPCPSTRIHHPSGFAQFTDCQNSQGDPSTSFRSRKVSVKGQEEFCDCDASDNFSELSDPIQTSLHTHRSLSGGSCLCNDDADPPKEPEKATCVSKQCQCSSNDELQGMQSAQAGTGYQTPEDLCGNRAMACCSRKERILMLMEKLTSPGCDCGISRPCLTQQLFRELTLLLRSEKDDAVAPADEASPPCLTFDECCTAQHAGNGEGDPVEKPPSKRELQRVECFHQLEEYLCKCFLPPAEPQIPETDIYAFELDPDFPPEPEPKSPKENDNMQPCQCPEQFFDIGNALTCSGDGLDDDFFRDCEDDEEVKKFESKDDGKATTEPDLCQSLKAFIDKELQEILAEEANKEGEEVEEKTPSTVLREICERPTTVASPEEEEKPEQIECPFAGMVSCPPWTTQQTDWAAFPYNLDPCAQKESPSPAPRGPRTGLLPEGPLSPDMRNLCEQLLRKALKDCGLCGGDDVYSCEDDVCEECALNCEECPEDCPLEQCEECDDECPPEQQGCLCCHCRALICDEECKTVANTLRSGMCDPLCEMKYFIDSIIVDMHAMDCVLGNKKAKPKHLKATDSTNRGGPGDSFPVKIKSVWPLGNTALYVQWQLEDCRAVAGYEIYLDGHLTNRFYSFRHEAGVITNADVTNPHQVVVRAVAVGQEFPGEGPGKDATQECGCQAVALAHPELKAGAQRPWSPSLYYYDPNQLPPAEDVQPC l63-pif2-1 Interaction between deletion mutants of L63 with PIF2 detected by Y2H. 146501 N/A 146505 unspecified role unspecified role prey prey N/A 146506 unspecified role unspecified role bait bait physical association physical association false false false Residues 54-89 of L63 important for the interaction. l63-pif1b-2 Interaction of deletions of L63 with PIF1B detected by Y2H. 146501 N/A 146506 unspecified role unspecified role bait bait N/A 146507 unspecified role unspecified role prey prey physical association physical association false false false Residues 54-89 of L63 important for the interaction. l63-pif1b-3 Interaction of deletions of PIF1B with L63 detected by Y2H. 146501 N/A 146507 unspecified role unspecified role prey prey N/A 146506 unspecified role unspecified role bait bait physical association physical association false false false Region between AA 788-815 involved in interaction. l63-pif2-3 Interaction of L63 with PIF2 detected by Y2H. 146501 N/A 146505 unspecified role unspecified role prey prey N/A 146506 unspecified role unspecified role bait bait physical association physical association false false false AA 1-64 of PIF2 Involved in the interaction. The construct has 15 CCXZ repeats. l63-pif1b-1 Interaction detected by Y2H. 146502 N/A 146507 unspecified role unspecified role prey prey N/A 146506 unspecified role unspecified role bait bait physical association physical association false false false l63-pif2-2 Interaction of L63 with PIF2 detected by Y2H. 146502 N/A 146505 unspecified role unspecified role prey prey N/A 146506 unspecified role unspecified role bait bait physical association physical association false false false l63-pif1b-4 Interaction detected by affinity chromatography. 146503 N/A 146507 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 146506 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association false false false PIF1B is his tagged. AA 756-845 of PIF1B used for peptide affinity purification. l63-pif1b-5 Interaction detected by CoIP. 146504 N/A 146506 unspecified role unspecified role bait bait N/A 146507 unspecified role unspecified role prey prey physical association physical association false false false