MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint holm-2002-1 Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Only protein-protein interactions imex curation imex curation holm-2002-2 Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. A directed yeast two hybrid assay was used in this study. Figure 1 (c). xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Only protein-protein interactions imex curation imex curation holm-2002-3 Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. allce Allium cepa (Onion) Onion fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. The FRET analysis was carried out in onion epidermis cells. Constructs were introduced into the cells by partical bombardment. Figure 2 (f). xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Only protein-protein interactions imex curation imex curation holm-2002-4 Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. Figure 8 (b). xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Only protein-protein interactions imex curation imex curation cop1_arath E3 ubiquitin-protein ligase COP1 COP1 Constitutive photomorphogenesis protein 1 RING-type E3 ubiquitin transferase COP1 T21L14.11 At2g32950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEISTDPVVPAVKPDPRTSSVGEGANRHENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLTNNQLYPNFLLDKLLKKTSARHVSKTASPLDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQPNSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRHGDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSPDMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAA q8w191-1 Transcription factor HY5-like HYH BZIP64 HY5 homolog bZIP transcription factor 64 MKP6.27 At3g17609 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLQRPNGNSSSSSSHKKHKTEESDEELLMVPDMEAAGSTCVLSSSADDGVNNPELDQTQNGVSTAKRRRGRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNENTMLRKMLINTRPKTDDNH hy5_arath Transcription factor HY5 HY5 BZIP56 At5g11260 F2I11_150 Protein LONG HYPOCOTYL 5 bZIP transcription factor 56 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL hyh_arath Transcription factor HY5-like HY5 homolog HYH BZIP64 bZIP transcription factor 64 MKP6.27 At3g17609 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLQRPNGNSSSSSSHKKHKTEESDEELLMVPDMEAAGSTCVLSSSADDGVNNPELDQTQNGVSTAKRRRGRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNENTMLRKMLINTRPKTDDNH cop1-hyh-3 Yeast 2 hybrid analysis reveals interaction between COP1 protein and the HYH protein 104375 N/A 104379 unspecified role unspecified role bait bait N/A 104380 unspecified role unspecified role prey prey physical association physical association false false false cop1-hyh-1 A directed yeast 2 hybrid analysis reveals interaction between COP1 protein and the HYH protein 104376 N/A 104379 unspecified role unspecified role bait bait P43254:p.Lys422Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P43254:p.Arg465Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P43254:p.Trp467Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P43254:p.Lys550Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P43254:p.Gly524Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 104380 unspecified role unspecified role prey prey Q8W191-1:p.[Val31Ala;Pro32Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false cop1-hyh-2 FRET analysis reveals interaction between COP1 protein and the HYH protein 104377 N/A 104379 unspecified role unspecified role neutral component neutral component gfp-tag blue version of GFP gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 104380 unspecified role unspecified role neutral component neutral component gfp-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 2F hy5-hyh-3 Anti tag COIP analysis reveals interaction between HY5 protein and the HYH protein 104378 N/A 104381 unspecified role unspecified role bait bait N/A 104382 unspecified role unspecified role prey prey physical association physical association false false false