IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mehra-1999-1 Negative regulation of male development in Caenorhabditis elegans by a protein-protein interaction between TRA-2A and FEM-3. in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Mehra A., Gaudet J., Heck L., Kuwabara PE., Spence AM. Fig 3 andrew.spence@utoronto.ca Genes Dev. (0890-9369) 1999 mehra-1999-2 Negative regulation of male development in Caenorhabditis elegans by a protein-protein interaction between TRA-2A and FEM-3. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mehra A., Gaudet J., Heck L., Kuwabara PE., Spence AM. Fig 3 andrew.spence@utoronto.ca Genes Dev. (0890-9369) 1999 tra2_caeel Sex-determining transformer protein 2 tra-2 C15F1.3 Ce-Tra-2 protein protein caeel Caenorhabditis elegans MKLKYNKLLVSVVIVTFVTFGLLLAECFGKSIDYQEKSIFPSFVSQGFFETRTNNEEYIIEKIAQTQENGVDMRSTLHFTQHGYLLNNISNLKIKFRQKTYTLNDVCFKPHITIFQQSSSSDQNEYPHYIQRLLLEMQRLSPCLIVTPLNCFYDIYRIHGEISNWNKNTDFLNRRLRNSYIEAIGENDERPYVKSNYGPSLIKSWADHMFDLPSKSFTNSTKDALFQKIKLWLLSIEPRQKTCAASIHSCDTPLDSEHYFNICTDMQSVDNFAEKKTKFKLEDVDEEFAMNLDCVDDQEQFIEWMQELEIRKMYSHVTEKPDYPNVVNQTCDKIFHDLNSTGIEFFDGSRSFSSTKSQFDTMQTEIVLLTPEMLLSAMQHSDFVNGFESIWTIEKAEELIHEFRLALKEETEKFKENRMSKMIRVTSRVLDNTVTTKLQSFSEKQTIHFVVNVHSLIVILFTIFVWSGAPLRSAFMFFVRDALTCLLFCFVCSTDGVIVLDTELIKYIIVLTLANLYFTTRSSFCTERLSRCIQREKRFPINSNFASLITVDTMTDSRQIQYFLSTVTKYQAAQDSYSNELFERFPKNWGCTSILIFPIVFVYWYFIDSNFDKICVSVLPSFCLAAGEELFAKNMFWKEREAMQAKQRLENEEQAESITGSSLEKLFAGNKPVSNTDKANIVKKSSIIRNQKPCLQDLSPGTYDVSNFMKYPHQASRIFREKIIGLYLRILKLRTLGVILCIPAILLIVISIGLLFIPVKRETLHTDSKQDDIFIEFEIFNFSTNWKIVNQNLKQFSEDIESIGTLYTISNWQKSFERFEQETNKNASAEWNILFKWINDEPINSAVTLFSEKSSGNQTIANPFKFRLRYGFDAKNETTVIEIVQKIDELLSKCSKNLSPKAVGVLYEHYHRIAVVWNLFAFNQLTTAGIFIILLSIITFIFAITPTIKATFLFSLLVVGTQIEVAALVHLFSLDHHQIYTNLALFAGFLAAWDPFCALLRYRRRILYKSETRRTPELASKRRVLLPIVATADIAQFFVLLITAFSILAIICSIVPELNIFFVPTVILIVIQIVAVFNSIIVSIATKQMFESEVRHYLHRDLRGSTTAVRVYNLVQKQRLASSLDEPQVELDEFSIKRSSPPCRYYAPPPKYSCKKRSRSSDEDEDSDPNQPGPSNRRSPKTGNKRVRGNGDNTELYIPNRYELIVSGKSVGGNTSAAWNGPGSTLEQNMNALEECFELGVDEYDFDEHDGDEGCELVQDMLDRERNLMNKRSTAQRRESRNIEKMKKSQENLDKEKSEEKISESKKNQDDSIESPNLPGTPANLPVDEPLPPVGRLYIVEHVLPEEYRRDPLTEPPSMEDCIRAHSDPNLPPHPRADQYPASFTRPMVEYCEDIYWTHRTGQLPPGLQVPRRPYDYYHITERTPPPEDLNWVPPAESPPIPIPQQAFDLLEERRRNHREQQDEAREGDLSDPEV fem3_caeel Sex-determination protein fem-3 Feminization of XX and XO animals protein 3 Ce-FEM-3 C01F6.4 fem-3 protein protein caeel Caenorhabditis elegans MEVDPGSDDVEADRETRAQKLKLKRNVKFRAQMRRFDEYCGVTNLTVDDLNWPLISGIPLQRQRLTGATYYDDSLLDQNPWDEFSIDRFLEITSIQLITAGAGYERNDEITRFVFQRTMKTIVTYCNFMYDLARRNGKVQITRFELQDLIHRDEFRFYMYFRQFLPNPDPNCTAFSNHYTSLLHTLYFNIPGMPQFWNNSQMYNYAATRGQRLVQNIAAFYPPEYFWNEDESKYHTTFVVPRGTEFSKFYARRFHEALGMPPLENEIITVLDWLAKLCILEIVYHTTIWCDITGFGGLPRIEHYRLAMENVEDIIFDLAIDDFSISRLQLQISPFEISRYSPLDVSGYYETIKRKKDIEEYQNRFYEVHYSDDVRIMNVYATDCSRKR fem3-tra2-2 interaction detected by Coimmunoprecipitation in C.elegans 143779 N/A 143781 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 143782 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag monoclonal western monoclonal antibody western blot n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro physical association physical association false false false Shows that the c-terminal domain of tra-2 can associate with fem-3. fem3-tra2-1 interaction detected Y2H in C.elegans 143780 N/A 143782 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 143781 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false A 141 AA region of tra-2 was able to interact with 1-343AA of fem-3. Deletion of AA 1-69 or 309-388 in fem-3 inhibited binding to tra-2.