IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ irene-2020-2 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Irene Lui, Xin X. Zhou, Shion A. Lim, Susanna K. Elledge, Paige Solomon, Nicholas J. Rettko, Beth Shoshana Zha, Lisa L. Kirkemo, Josef A. Gramespacher, Jia Liu, Frauke Muecksch, Julio Cesar Cetrulo Lorenzi, Fabian Schmidt, Yiska Weisblum, Davide F. Robbiani, Michel C. Nussenzweig, Theodora Hatziioannou, Paul D. Bieniasz, Oren S. Rosenburg, Kevin K. Leung and James A. Wells Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review Biotinylated Spike or Spike RBD protein were immobilized on the streptavidin biosensor. After blocking with biotin, purified ACE2 proteins in solution was used as the analyte Spike-RBD and ACE2 constructs have an AviTag™ BirA biotinylation acceptor sequence, leading to enzymatic biotinylation by BirA Expi293 cells. jim.wells@ucsf.edu Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus irene-2020-3 in vitro In vitro split luciferase complementation split luciferase complementation predetermined predetermined participant predetermined Irene Lui, Xin X. Zhou, Shion A. Lim, Susanna K. Elledge, Paige Solomon, Nicholas J. Rettko, Beth Shoshana Zha, Lisa L. Kirkemo, Josef A. Gramespacher, Jia Liu, Frauke Muecksch, Julio Cesar Cetrulo Lorenzi, Fabian Schmidt, Yiska Weisblum, Davide F. Robbiani, Michel C. Nussenzweig, Theodora Hatziioannou, Paul D. Bieniasz, Oren S. Rosenburg, Kevin K. Leung and James A. Wells Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review Spike-RBD and ACE2 constructs have an AviTag™ BirA biotinylation acceptor sequence, leading to enzymatic biotinylation by BirA expressing Expi293 cells. jim.wells@ucsf.edu Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus irene-2020-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Irene Lui, Xin X. Zhou, Shion A. Lim, Susanna K. Elledge, Paige Solomon, Nicholas J. Rettko, Beth Shoshana Zha, Lisa L. Kirkemo, Josef A. Gramespacher, Jia Liu, Frauke Muecksch, Julio Cesar Cetrulo Lorenzi, Fabian Schmidt, Yiska Weisblum, Davide F. Robbiani, Michel C. Nussenzweig, Theodora Hatziioannou, Paul D. Bieniasz, Oren S. Rosenburg, Kevin K. Leung and James A. Wells Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review jim.wells@ucsf.edu Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2-s-52 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 1.0 N/A 3729888 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 1.0 direct interaction direct interaction false false false 3729884 Fig. 2a s-ace2-30 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 1.0 N/A 3729888 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 direct interaction direct interaction false false false 3729884 Fig. 2b s-ace2-31 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 N/A 3729888 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 1.0 direct interaction direct interaction false false false 3729884 Fig. 2d s-ace2-32 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 N/A 3729888 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2e s-ace2-33 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 N/A 3729888 unspecified role unspecified role bait bait purified purified ectodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC2:p.Arg682_Arg685delinsAla mutation mutation certain certain sequence position certain certain certain sequence position certain false P0DTC2:p.Lys986_Val987delinsProPro mutation mutation certain certain sequence position certain certain certain sequence position certain false T4 foldon trimerization domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 3.0 direct interaction direct interaction false false false 3729884 Fig. 2f ACE2-Fc concentration range:100 nM to 3.125 nM s-ace2-35 3729884 N/A 3729887 unspecified role unspecified role prey prey purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 N/A 3729888 unspecified role unspecified role bait bait purified purified ectodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC2:p.Arg682_Arg685delinsAla mutation mutation certain certain sequence position certain certain certain sequence position certain false P0DTC2:p.Lys986_Val987delinsProPro mutation mutation certain certain sequence position certain certain certain sequence position certain false T4 foldon trimerization domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 3.0 direct interaction direct interaction false false false 3729884 Supplementary Figure 3a ACE2-Fc concentration range: 100 nM to 3.125 nM at 2-fold dilutions s-ace2-34 3729885 N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false smBIT tag luciferase tag luciferase tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lgBIT luciferase tag luciferase tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729888 unspecified role unspecified role ancillary ancillary purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f physical association physical association false false false Fig. 3e The Spike protein is being pulled-down by biotin tag ace2-ace2-2 3729885 N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false smBIT tag luciferase tag luciferase tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lgBIT luciferase tag luciferase tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729888 unspecified role unspecified role ancillary ancillary purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f physical association physical association false false false Supplementary Figure 4a-b ace2-ace2-3 3729885 N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false smBIT tag luciferase tag luciferase tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729887 unspecified role unspecified role neutral component neutral component purified purified protease domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lgBIT luciferase tag luciferase tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3729888 unspecified role unspecified role ancillary ancillary purified purified ectodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 foldon trimerization domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC2:p.Lys986_Val987delinsProPro mutation mutation certain certain sequence position certain certain certain sequence position certain false P0DTC2:p.Arg682_Arg685delinsAla mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f physical association physical association false false false Fig. 3f s-34 3729886 N/A 3729888 unspecified role unspecified role neutral component neutral component purified purified T4 foldon trimerization domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ectodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC2:p.Lys986_Val987delinsProPro mutation mutation certain certain sequence position certain certain certain sequence position certain false P0DTC2:p.Arg682_Arg685delinsAla mutation mutation certain certain sequence position certain certain certain sequence position certain false c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 3.0 direct interaction direct interaction false false false Fig. 1d