UniProt UniProt http://www.uniprot.org henderson-1997-2 Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. in vitro In vitro pull down pull down weight silver stain molecular weight estimation by silver staining weight silver stain Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a J. Mol. Biol. (0022-2836) 1997 IMEx imex curation Only protein-protein interactions henderson-1997-4 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight silver stain molecular weight estimation by silver staining weight silver stain Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a J. Mol. Biol. (0022-2836) 1997 imex curation Only protein-protein interactions henderson-1997-1 Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Henderson B.R., Percipalle P. Virus - Publications including interactions involving viral proteins Mixed 35S-labelled Rev and unlabelled Rev (to provide excess of Rev over importin) have been used in a buffer containing 35S-labelled importin-alpha Origin of HIB-1 Rev according Zemmel et al, 1996, PubMed 8637008. Purification of importin alpha and beta according Percipalle et al, 1997, PubMed 9102465. n/a J. Mol. Biol. (0022-2836) 1997 IMEx imex curation Only protein-protein interactions ima1_human Importin subunit alpha-1 KPNA2 RCH1 SRP1 Karyopherin subunit alpha-2 SRP1-alpha RAG cohort protein 1 protein protein human Homo sapiens Human MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF rev_hv1br Protein Rev rev ART/TRS Anti-repression transactivator Regulator of expression of viral proteins protein protein hv1br Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI) HIV-1 MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE imb1_human Importin subunit beta-1 Karyopherin subunit beta-1 Nuclear factor p97 Importin-90 KPNB1 NTF97 Pore targeting complex 97 kDa subunit protein protein human Homo sapiens Human MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA rev-kpna2-1 1787870 n/a 1787873 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) n/a 1787874 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted physical association physical association false false false 4c Not changed by HIV-1 Rev mutant arg_arg38-39asp_leu rev-kpnb1-1 1787870 n/a 1787874 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P04620:p.Arg38_Arg39delinsAspLeu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted n/a 1787875 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false 5 and 7a and b tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound) kpnb1-rev-1 1787871 N/A 1787873 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) N/A 1787874 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted N/A 1787875 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) association association false false false 6 tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound) kpnb1-rev-2 1787871 N/A 1787874 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) a Rev arg38asp_arg39leu mutant also interacted N/A 1787875 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false 6 tRNA (in a twofold excess) KPNA2/importin-alpha IBB domain (InterPro; IPR00265) RAN (GTP-bound) rev-kpna2-2 1787872 n/a 1787873 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1787874 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 4a