IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2021-4 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins FRET based assay:On cleavage of the peptide by the protease, the quencher (DNP) and fluorophore (MCA) are separated resulting in a large increase in MCA fluorescence. 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus li-2021-3 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins Fluorescence quenching was carried out at various temperatures. 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus mca-avlqsgfr-lys(dnp)-lys-nh2 MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AVLQSGFR p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gc-376 GC-376 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) heparin-rep-2 4110881 N/A 4110883 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110884 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 4110885 inhibitor inhibitor neutral component neutral component purified purified pig-intestinal mucosa pig Pig intestinal mucosa direct interaction direct interaction false false false 4110881 4110881 3 C/D, 4 A-D heparin-mca_avlqsgfr_lys(dnp)_lys_nh2-1 4110881 N/A 4110883 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110884 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4110881 4110881 3 A/B, 4 gc_376-mca_avlqsgfr_lys(dnp)_lys_nh2-1 4110881 N/A 4110883 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110884 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 4110886 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 4110881 4 E/F heparin-rep-1 4110882 N/A 4110884 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 4110885 unspecified role unspecified role neutral component neutral component purified purified pig-intestinal mucosa pig Pig intestinal mucosa direct interaction direct interaction false false false 2 In the text the Dissociation constants are given as 1.66 uM at 25 deg.C and and 3.16 at 35 deg.C -average 2.41 uM. However results in Table 1 give X10 higher values for Kd at these temperatures. A higher concentration of Sodium chloride did not significantly influence the interaction of heparin with Mpro.