IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ savastano-2020-1 in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Nature communications 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus savastano-2020-2 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Nature communications 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus savastano-2020-3 in vitro In vitro frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Markus.Zweckstetter@dzne.de Nature communications 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus savastano-2020-4 in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Nature communications 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus savastano-2020-5 in vitro In vitro protein kinase assay protein kinase assay ms participant Identification by mass spectrometry ms participant Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Markus.Zweckstetter@dzne.de Nature communications 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA poly(uridylic acid) poly(uridylic acid) 5'-Uridylic acid, homopolymer Poly U poly(U) Polyuridylic acid Uracil polynucleotide small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) srpk1_human SRSF protein kinase 1 Serine/arginine-rich protein-specific kinase 1 SFRS protein kinase 1 SRPK1 protein protein human Homo sapiens Human MERKVLALQARKKRTKAKKDKAQRKSETQHRGSAPHSESDLPEQEEEILGSDDDEQEDPNDYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPNREMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS n-poly(uridylic acid)-1 3959208 N/A 3959213 unspecified role unspecified role neutral component neutral component purified purified region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3959214 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1B, 4C, Supplementary Fig. 1C At 50 µM protein concentration, the addition of 1 µM polyU, strongly increased turbidity. n-poly(uridylic acid)-2 3959209 N/A 3959213 unspecified role unspecified role neutral component neutral component purified purified region alexa label alexa label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3959214 unspecified role unspecified role neutral component neutral component region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1C /(D), 4D n-poly(uridylic acid)-3 3959210 N/A 3959213 unspecified role unspecified role neutral component neutral component purified purified region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3959214 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1E, 4E, Supplementary Fig. 1D Fluorescence did not fully recover (Fig. 1e), i.e., ~60% of NSARS-CoV-2 had transformed into an immobile species. n-poly(uridylic acid)-4 3959211 N/A 3959213 unspecified role unspecified role neutral component neutral component purified purified SR-rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Ser188 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3959214 unspecified role unspecified role neutral component neutral component region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig. 3d, 4b, Supplementary Fig. 4 n-srpk1-3 3959212 N/A 3959213 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Ser188 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3959215 enzyme enzyme neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 9inse 9inse direct interaction direct interaction false false false Supplementary Fig. 8 Supp. figure S8 shows the full length protein based on M. Wts shown. It is stated that:- " identified masses indicate 4 to 5 sites being phosphorylated";however it is not stated which of the many possible sites these were.