IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2020-9 in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Thermal shift of SARS-CoV-2 macro domain on binding with ADPR, ADP, and AMP binding was detected by intrinsic fluorescence from aromatic residues. andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-11 in vitro In vitro de-adp-ribosylation assay de-ADP-ribosylation assay tag visualisation tag visualisation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins PARP10 has been auto-mono-ADPribosylated with biotin-labelled ADP andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-13 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay antibody detection identification by antibody antibody detection Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-8 in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins DSF Assay (Differential scanning fluorimetry): SYPRO Orange binds nonspecifically to hydrophobic surfaces, and water strongly quenches its fluorescence. When the protein unfolds, the exposed hydrophobic surfaces bind the dye, resulting in an increase in fluorescence. andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-10 in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins Thermal transition profiles of the protein with or without ADPR were monitored by using Circular Dichroism. andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus lin-2020-12 in vitro In vitro adp ribosylase adp ribosylase assay adp ribosylase tag visualisation tag visualisation Lin MH., Chang SC., Chiu YC., Jiang BC., Wu TH., Hsu CH. Virus - Publications including interactions involving viral proteins andyhsu@ntu.edu.tw ACS infectious diseases 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus adp-d-ribose ADP-D-ribose (Rib5)ppA 5-(adenosine 5'-pyrophosphoryl)-D-ribose A5'pp5Rib adenosine 5'-(trihydrogen diphosphate), P'-5-ester with D-ribose adenosine 5'-diphosphoribose adenosine 5'-pyrophosphate, 5'-5-ester with D-ribofuranose adenosine diphosphate ribose Adenosine diphosphate ribose AdoPPRib ADP ribose ADP-D-ribose ADP-Rib ADP-riboses ADPribose D-ribofuranose, 5-5'-ester with adenosine 5'-(trihydrogen pyrophosphate) ribose adenosinediphosphate adenosine 5'-[3-(D-ribofuranos-5-O-yl) dihydrogen diphosphate] small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0dtc1-pro_0000449637 Papain-like protease nsp3 ORF1a polyprotein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) amp Adenosine monophosphate 5'-Adenosine monophosphate 5'-Adenylic acid 5'-AMP 5'-O-phosphonoadenosine adenosine 5'-(dihydrogen phosphate) Adenosine 5'-monophosphate Adenosine 5'-phosphate ADENOSINE MONOPHOSPHATE adenosine phosphate Adenosine-5'-monophosphoric acid adenosine-5'P adenosini phosphas Adenylate Adenylic acid Ado5'P AMP fosfato de adenosina pA PAdo phosphate d'adenosine 5'-adenylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) par10_human Protein mono-ADP-ribosyltransferase PARP10 Poly [ADP-ribose] polymerase 10 ADP-ribosyltransferase diphtheria toxin-like 10 PARP10 protein protein human Homo sapiens Human MVAMAEAEAGVAVEVRGLPPAVPDELLTLYFENRRRSGGGPVLSWQRLGCGGVLTFREPADAERVLAQADHELHGAQLSLRPAPPRAPARLLLQGLPPGTTPQRLEQHVQALLRASGLPVQPCCALASPRPDRALVQLPKPLSEADVRVLEEQAQNLGLEGTLVSLARVPQARAVRVVGDGASVDLLLLELYLENERRSGGGPLEDLQRLPGPLGTVASFQQWQVAERVLQQEHRLQGSELSLVPHYDILEPEELAENTSGGDHPSTQGPRATKHALLRTGGLVTALQGAGTVTMGSGEEPGQSGASLRTGPMVQGRGIMTTGSGQEPGQSGTSLRTGPMGSLGQAEQVSSMPMGSLEHEGLVSLRPVGLQEQEGPMSLGPVGSAGPVETSKGLLGQEGLVEIAMDSPEQEGLVGPMEITMGSLEKAGPVSPGCVKLAGQEGLVEMVLLMEPGAMRFLQLYHEDLLAGLGDVALLPLEGPDMTGFRLCGAQASCQAAEEFLRSLLGSISCHVLCLEHPGSARFLLGPEGQHLLQGLEAQFQCVFGTERLATATLDTGLEEVDPTEALPVLPGNAHTLWTPDSTGGDQEDVSLEEVRELLATLEGLDLDGEDWLPRELEEEGPQEQPEEEVTPGHEEEEPVAPSTVAPRWLEEEAALQLALHRSLEPQGQVAEQEEAAALRQALTLSLLEQPPLEAEEPPDGGTDGKAQLVVHSAFEQDVEELDRALRAALEVHVQEETVGPWRRTLPAELRARLERCHGVSVALRGDCTILRGFGAHPARAARHLVALLAGPWDQSLAFPLAASGPTLAGQTLKGPWNNLERLAENTGEFQEVVRAFYDTLDAARSSIRVVRVERVSHPLLQQQYELYRERLLQRCERRPVEQVLYHGTTAPAVPDICAHGFNRSFCGRNATVYGKGVYFARRASLSVQDRYSPPNADGHKAVFVARVLTGDYGQGRRGLRAPPLRGPGHVLLRYDSAVDCICQPSIFVIFHDTQALPTHLITCEHVPRASPDDPSGLPGRSPDT poa1_yeast ADP-ribose 1''-phosphate phosphatase POA1 [Protein ADP-ribosylglutamate] hydrolase YBR0304 YBR022W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNITYVKGNILKPKSYARILIHSCNCNGSWGGGIAYQLALRYPKAEKDYVEVCEKYGSNLLGKCILLPSYENSDLLICCLFTSSFGGSSHGEKQSILNYTKLALDKLKTFREAKDKTRTSEDSIGDYLNGHIKYPIGEYKLEMPQINSGIFGVPWKETERVLEEFSGDMSFTVYQL poly(adpribose) Poly(ADPribose) Poly(ADPribose) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) chd1l_human Chromodomain-helicase-DNA-binding protein 1-like CHD1L ALC1 Amplified in liver cancer protein 1 protein protein human Homo sapiens Human MERAGATSRGGQAPGFLLRLHTEGRAEAARVQEQDLRQWGLTGIHLRSYQLEGVNWLAQRFHCQNGCILGDEMGLGKTCQTIALFIYLAGRLNDEGPFLILCPLSVLSNWKEEMQRFAPGLSCVTYAGDKEERACLQQDLKQESRFHVLLTTYEICLKDASFLKSFPWSVLVVDEAHRLKNQSSLLHKTLSEFSVVFSLLLTGTPIQNSLQELYSLLSFVEPDLFSKEEVGDFIQRYQDIEKESESASELHKLLQPFLLRRVKAEVATELPKKTEVVIYHGMSALQKKYYKAILMKDLDAFENETAKKVKLQNILSQLRKCVDHPYLFDGVEPEPFEVGDHLTEASGKLHLLDKLLAFLYSGGHRVLLFSQMTQMLDILQDYMDYRGYSYERVDGSVRGEERHLAIKNFGQQPIFVFLLSTRAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAHRIGQNKSVKVIRLIGRDTVEEIVYRKAASKLQLTNMIIEGGHFTLGAQKPAADADLQLSEILKFGLDKLLASEGSTMDEIDLESILGETKDGQWVSDALPAAEGGSRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLLEKASQEGRSLRNKGSVLIPGLVEGSTKRKRVLSPEELEDRQKKRQEAAAKRRRLIEEKKRQKEEAEHKKKMAWWESNNYQSFCLPSEESEPEDLENGEESSAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSGHWGRGGLFTALEKRSAEPRKIYELAGKMKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLSGIKMAALEEGLKKIFLAAKKKKASVHLPRIGHATKGFNWYGTERLIRKHLAARGIPTYIYYFPRSKSAVLHSQSSSSSSRQLVP nad+ nicotinamide adenine dinucleotide - oxidised form small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adp_d_ribose-pro_0000449637-1 3933050 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3d adp-pro_0000449637-1 3933050 N/A 3933060 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3d amp-pro_0000449637-1 3933050 N/A 3933061 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3d adp_d_ribose-rep-6 3933051 N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 direct interaction direct interaction false false false Table 2 adp_d_ribose-p0dtc1_pro_0000449637-1 3933051 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false Table 2 parp10-pro_0000449637-1 3933052 N/A 3933062 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 3933059 enzyme enzyme neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction false false false Fig. 4a-b parp10-pro_0000449637-2 3933052 N/A 3933062 enzyme target enzyme target substrate substrate neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3933063 enzyme enzyme neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind range range range range false de-adp-ribosylation reaction de-ADP-ribosylation reaction false false false Fig. 4a-b Positive control adp_d_ribose-pro_0000449637-4 3933053 N/A 3933064 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role bait bait purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4c chd1l-adp_d_ribose-1 3933053 N/A 3933065 unspecified role unspecified role bait bait purified purified macro domain sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 3933064 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig. 4c adp_d_ribose-rep-7 3933054 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC1-PRO_0000449637:p.[Val228_Glu229delinsIleGln;Phe360Asn] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 3933054 Table 1, Fig. 3a, Supp. 4B. adp_d_ribose-pro_0000449637-2 3933055 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3933055 Table 1, Fig. 3c adp_d_ribose-pro_0000449637-3 3933056 N/A 3933058 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933059 unspecified role unspecified role neutral component neutral component purified purified macro domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2b biotin-parp10-1 3933057 N/A 3933066 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3933062 putative self putative self neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind range range range range false adp ribosylation adp ribosylation reaction adp ribosylation false false false Fig. 4a