IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jo-2020-1 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins FRET based assay, with substrate Dabcyl-KTSAVLQSGFRKME-Edans peptide. A complementary fluorophore (EDANS) and quencher (DABCYL) were attached to either end of a cleavable enzyme substrate (KTSAVLQSGFRKME). Upon enzymatic cleavage of the substrate, the fluorophore and quencher are separated and emission is restored, producing a signal that is directly correlated to the activity of the enzyme. dhshin55@ewha.ac.kr Journal of enzyme inhibition and medicinal chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jo-2020-2 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Jo S., Kim S., Kim DY., Kim MS., Shin DH. Virus - Publications including interactions involving viral proteins dhshin55@ewha.ac.kr Journal of enzyme inhibition and medicinal chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus peptide substrate peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KTSAVLQSGFRKME p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 baicalin baicalin 5,6,7-trihydroxyflavone 7-O-beta-D-glucuronide 5,6-dihydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranosiduronic acid 7-D-glucuronic acid-5,6-dihydroxyflavone Baicalein 7-O-glucuronide Baicalin 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 8-hydroxykaempferol 8-hydroxykaempferol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pectolinarin Pectolinarin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) peptide substrate-rep-3 3729719 N/A 3729721 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3729719 Figure 2 peptide substrate-rep-4 3729719 N/A 3729723 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729721 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3729719 Figure 2 8_hydroxykaempferol-peptide substrate-1 3729719 N/A 3729724 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729721 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3729719 Figure 2 pectolinarin-peptide substrate-1 3729719 N/A 3729725 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729721 enzyme target enzyme target substrate substrate neutral component neutral component EDANS fluorophore fluorophore c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Dabycl quencher dye labelled dye label dye labelled n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3729719 Figure 2 pectolinarin-peptide substrate-2 3729720 N/A 3729725 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3 8_hydroxykaempferol-peptide substrate-2 3729720 N/A 3729724 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3 baicalin-peptide substrate-1 3729720 N/A 3729723 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3729722 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3