HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html kumar-2013-1 Human La protein interaction with GCAC near the initiator AUG enhances hepatitis C Virus RNA replication by promoting linkage between 5' and 3' untranslated regions. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kumar A., Ray U., Das S. Virus - Publications including interactions involving viral proteins sdas@mcbl.iisc.ernet.in J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation q99ib8-pro_0000045603 RNA-directed RNA polymerase protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SMSYSWTGALITPCSPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRAKKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLEEACQLTPPHSARSKYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDPAKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQRVEYLLKAWAEKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPEEARTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKALAACKAAGIVAPTMLVCGDDLVVISESQGTEEDERNLRAFTEAMTRYSAPPGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWETVRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLNFEMYGSVYSVNPLDLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAVRASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEARLLDLSSWFTVGAGGGDIFHSVSRARPRSLLFGLLLLFVGVGLFLLPAR 2443 3033 la_human Lupus La protein SSB Sjoegren syndrome type B antigen La ribonucleoprotein La autoantigen protein protein human Homo sapiens Human MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ q99ib8-pro_0000045599 Serine protease/helicase NS3 protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV APITAYAQQTRGLLGAIVVSMTGRDRTEQAGEVQILSTVSQSFLGTTISGVLWTVYHGAGNKTLAGLRGPVTQMYSSAEGDLVGWPSPPGTKSLEPCKCGAVDLYLVTRNADVIPARRRGDKRGALLSPRPISTLKGSSGGPVLCPRGHVVGLFRAAVCSRGVAKSIDFIPVETLDVVTRSPTFSDNSTPPAVPQTYQVGYLHAPTGSGKSTKVPVAYAAQGYKVLVLNPSVAATLGFGAYLSKAHGINPNIRTGVRTVMTGEAITYSTYGKFLADGGCASGAYDIIICDECHAVDATSILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHPDIEEVGLGREGEIPFYGRAIPLSCIKGGRHLIFCHSKKKCDELAAALRGMGLNAVAYYRGLDVSIIPAQGDVVVVATDALMTGYTGDFDSVIDCNVAVTQAVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRQGTYRYVSTGERASGMFDSVVLCECYDAGAAWYDLTPAETTVRLRAYFNTPGLPVCQDHLEFWEAVFTGLTHIDAHFLSQTKQAGENFAYLVAYQATVCARAKAPPPSWDAMWKCLARLKPTLAGPTPLLYRLGPITNEVTLTHPGTKYIATCMQADLEVMT 1031 1661 positive- sense rna HCV JFH-1 positive- sense RNA rna ribonucleic acid RNA rna hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV ns5b-la-1 1457364 n/a NS5B 1457365 unspecified role unspecified role prey prey n/a La 1457366 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5A Mutation in the GCAC motif (346-349) near the initiator AUG in the SLIV region of the HCV internal ribosomal entry site (IRES) decrease interaction ns5b-la-2 1457364 n/a La 1457366 unspecified role unspecified role prey prey n/a NS5B 1457365 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5B Mutation in the GCAC motif (346-349) near the initiator AUG in the SLIV region of the HCV internal ribosomal entry site (IRES) decrease interaction ns3-la-1 1457364 n/a NS3 1457367 unspecified role unspecified role prey prey n/a La 1457366 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7H Mutation in the GCAC motif (346-349) near the initiator AUG in the SLIV region of the HCV internal ribosomal entry site (IRES) decrease interaction rna-la-1 1457364 n/a 1457368 unspecified role unspecified role prey prey region mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a La 1457366 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3B and 5A Mutation in the GCAC motif (346-349) near the initiator AUG in the SLIV region of the HCV internal ribosomal entry site (IRES) decrease interaction