HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html huang-2013c-1 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. in vitro In vitro pull down pull down de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation huang-2013c-2 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation huang-2013c-4 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation huang-2013c-5 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation huang-2013c-6 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation huang-2013c-7 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw J. Virol. (0022-538X) 2013 imex curation Only protein-protein interactions imex curation q99ib8-pro_0000045603 RNA-directed RNA polymerase protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SMSYSWTGALITPCSPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRAKKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLEEACQLTPPHSARSKYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDPAKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQRVEYLLKAWAEKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPEEARTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKALAACKAAGIVAPTMLVCGDDLVVISESQGTEEDERNLRAFTEAMTRYSAPPGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWETVRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLNFEMYGSVYSVNPLDLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAVRASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEARLLDLSSWFTVGAGGGDIFHSVSRARPRSLLFGLLLLFVGVGLFLLPAR 2443 3033 fas_human Fatty acid synthase FASN FAS Type I fatty acid synthase protein protein human Homo sapiens Human MEEVVIAGMSGKLPESENLQEFWDNLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSRFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPDSLRGTHTGVWVGVSGSETSEALSRDPETLVGYSMVGCQRAMMANRLSFFFDFRGPSIALDTACSSSLMALQNAYQAIHSGQCPAAIVGGINVLLKPNTSVQFLRLGMLSPEGTCKAFDTAGNGYCRSEGVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDIQEQLIRSLYQSAGVAPESFEYIEAHGTGTKVGDPQELNGITRALCATRQEPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALLDGRLQVVDQPLPVRGGNVGINSFGFGGSNVHIILRPNTQPPPAPAPHATLPRLLRASGRTPEAVQKLLEQGLRHSQDLAFLSMLNDIAAVPATAMPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDLLSCMGLRPDGIVGHSLGEVACGYADGCLSQEEAVLAAYWRGQCIKEAHLPPGAMAAVGLSWEECKQRCPPGVVPACHNSKDTVTISGPQAPVFEFVEQLRKEGVFAKEVRTGGMAFHSYFMEAIAPPLLQELKKVIREPKPRSARWLSTSIPEAQWHSSLARTSSAEYNVNNLVSPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIIPLMKKDHRDNLEFFLAGIGRLHLSGIDANPNALFPPVEFPAPRGTPLISPLIKWDHSLAWDVPAAEDFPNGSGSPSAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGVEQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGKVYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQGILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHIDPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGNLQLELAQVLAQERPKLPEDPLLSGLLDSPALKACLDTAVENMPSLKMKVVEVLAGHGHLYSRIPGLLSPHPLLQLSYTATDRHPQALEAAQAELQQHDVAQGQWDPADPAPSALGSADLLVCNCAVAALGDPASALSNMVAALREGGFLLLHTLLRGHPLGDIVAFLTSTEPQYGQGILSQDAWESLFSRVSLRLVGLKKSFYGSTLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADEDSSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQKVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSIRWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTSQDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEEAASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHTGGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFLKNVTFHGVLLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRYMAQGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLGGFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTSNISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKPKYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAMERICEKRRHEGLPGLAVQWGAIGDVGILVETMSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG ns5b-fasn-3 1454895 n/a NS5B 1454901 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a FASN 1454902 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma physical association physical association false false false Fig. 1A ns5b-fasn-1 1454896 n/a NS5B 1454901 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a FASN 1454902 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma physical association physical association false false false Fig. 1B fasn-ns5b-1 1454897 n/a FASN 1454902 unspecified role unspecified role bait bait n/a NS5B 1454901 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B fasn-ns5b-2 1454898 n/a NS5B 1454901 unspecified role unspecified role prey prey n/a FASN 1454902 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3C ns5b-fasn-4 1454899 n/a FASN 1454902 unspecified role unspecified role neutral component neutral component n/a NS5B 1454901 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3D ns5b-fasn-2 1454900 n/a FASN 1454902 unspecified role unspecified role neutral component neutral component n/a NS5B 1454901 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3D