HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html zaichick-2013-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins VP1/2 also interact with Dynein but the specific Dynein intermediate chain could not be traced g-smith3@northwestern.edu Cell host & microbe 2013 imex curation Only protein-protein interactions imex curation zaichick-2013-1 chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zaichick SV., Bohannon KP., Hughes A., Sollars PJ., Pickard GE., Smith GA. Virus - Publications including interactions involving viral proteins g-smith3@northwestern.edu Cell host & microbe 2013 imex curation Only protein-protein interactions imex curation g3g960_9alph UL36 protein protein pseudorabies virus pseudorabies virus MTADAVVVGYRNQYDPDLGPGSGVSCLRSSLSFLRLVFTNGVEAALTADAVDACLREGQAWTGPGGGMCAISELPNKIVYEGAGGAALHCVFSRVHGECNFFTPPAEGFQSTQITARAFFDGLWAQRAESFTLVVVGAVGLGLYRRGDVVYVFDPHGHGQITQAFVARLRPADVYAHVAGYAASELRWAGAFVFFLPHDQFAAVPALYGVGETYLVDEPYVERAVPSVHPMALPPDPAAAAPPTRGPGGIPVLAVAPVPAVPVSAPGGAPLPPSPRVQKRPSMPKRRRPPWPASQEDLSSTHTVGGRPSRKFRPRTREPMGFAVRELELAPLDLGPLQAHADELEEHVVACAGRSLFPGRAGLLERFAELLFGHLQDFLVENGARTHGEAPCAVAALFEPILGALPERGPAGDFVAATHMVLTEARALLDVAGDDASAPVAKLALVAARVARPANEAMHRALDALESEGSYAQLSACFMDAAARDPGGVYDQTVVARARALFARARARESRESQRALERARDVSALEAALEAAHANFDAVDAGAPGAGGAAEPNTRDVKERAAKLPRKLALMVGDAIREYFLRGAQYSAKAMLMDRNGAQRFRVANAAVAGLRRLVDSLAAAEERLRALDAAPAAHDGALLAQLLEAGEDVSTDERLGAWFALLAEAQAHGHLERRELDTLTRDVTQINERAAKRASADADLARFDAMDAALAAAPDEETRVRAMEDLLRHIRAMGGHVLSQETRARLARRDAELRTLVQAAQQRAAEQNDLYMRLRALLGDFATLGPAVDLLAHITATPVPGTVLAALWRLVARYRELLTSDPGALVGAGQPLGALVNALADNPPITRFFLAHADAVAAALADADTAEKAARAAGVLRAAVEHPELGFLEPLARQCEERAAALRESERVAALQAALSAAVAAAVEMLGRLRAQPDEGTLVEARHLALEVARAAAVEEPQLQKMALAARQRVGELEAAVAAVEARRAAEVERAQRQLWARAADAALRRAEQHAFDAAEFVRLRDVAADRGYDAAELRPAAERALGANAETVTTALDAVFAFNPHLPENAGAAAGPSLQALQRLTWLDDYAVAAPVIGSLFGADTEPLSRLARIAGGVLARAAAGMGRFSYYDAVGFVGADMAHVPRLAKYVDFYRRGFPEYEAELAALGARRADVLQAGGSRGAEICRALEEVTYVRAAADAARALDEGVHLHVPSTELLRGAVTYLERFDFARFERTAYAEAMRALVRRDLAAARDGLAAAEADQAAATERATRLLREVVDAAAARDRDGALELANLKNLLRVTPPPASLNIDRAASAEELVTQAALLLGAVEEAPELDVQAVEWLHQARLIIDTHPLTTRVDGRGPMDEFAERIEALQTLRQEADTLRQRLTAAEVAWDEAWEDFGRARGRAGASEDEARLAEARARALQTATMEVLALRSDAVYPRLPAKFVGRIESKYSARAGALGDFFRDAGAVEEDVRELDRMAARIGPELRHDALRALAAAFDARAERLPRWIPPKYAAYRELLTLRLQFYAAYGGAGPTPARPRASTAPCARLKQRVAARLGDRDVICTVREARSDADAVFPTVFLDDNNVPVEYQVCYRAVGDKLAAMLCTDEGAELRPPLTEGLMETATVAGMRLMNEIIHLRLNYEKARDTGFATFARFVRHRRADWDSAAKAAAETYAAVLATTLSRTFGALDRVAHPPLEPAVRLALPDLMVAAVAGEPTHLFNFTRVDLAGQHEYMAKTLDAVLDRALDAVDTLDAGARPPRRPLRDGQALFTLRVADWTEGRFSETELWAPWAHTETGQRLARAVPGALATFTVLARMCIPPRLLSALWSALQPEANAAYDAAVTERLDAAAHVREAAALGAPAPGDLYAPVGTGMTFTVTGSPPSSATRVSAMDLAAAAVLVGAPLVIAMENLDVYSPGSGLTLAVTLCDLRDPALHRGVSTDLSSWGERLLALDDNPIENACLAPQLERLSALIAARPLAGEPACLVLVDSQLAVSRVLWAPPEPPRRPGFTLEDDPLLREIPFLETTEDQLPEMDPADPLFTSIIMGDARVDPATSEELYGRPSFRNLGDPFPYAVSATPTRPATPIPGLPPELAGEDDGFVPDDDFGANPWLDLLGDDLVASALPLPPSPPVDPPTLPPLAPAAPAAPAAPPPAKPAEPTPAAKLAPPAPPPAKPAEPTPAAKLAPPAPPPAKPVETTPLTKPQPQPQQGPPAAHKKPAAGTKAAAAPKQQPREPAPKPHSSPRKIQPSLKARIEPPPPVINPPYPATAPAPETAPPEAPQAQPPAAAKPTPQPQGPPPPPQPPSAQAPPAQKPPAQPAAAATAATATTAPKANPQTQPPTRAQTQTAPPPPSAATAAAQVPPQPPSSQPAAKPRGAPPAPPAPPPPSAQTTLPRPAAPPAPPPPSAPAATPTPPAPGPAPSAKKSDGDRIVEPKAGAPPDVRDAKFGGKVAGTASPGRPFDDERYKAGRADKCEHTVGPDQHRTRSPPGTSDSEPASASSSAASTPSREAASSSSSPAGAPSDGARGGPPSAAGAAARAPPGAPGSGGQRRGDGGPAADASRGDQPAGHLPAAPVAQPLPSAVLRTKARTSVGIRDHSRRKSMALVPRRPVSAPRQTGGLPPAAQPEGRPPGGLEPRPPQAQAQAPATAPTGPPAAAAAAAATAAVPAPGVGPGGPVRRAAGPRPPAGHLRLHIPPPQPVQLEGVVVPLPASPETPAPAQTQPPRSPLAAPTSLAAPSEIERPAASAVVAAATTTTTSSSSSATTSSAPAPPPSRPAPPPSRPAPPPSRPAPPPPAPPPSRPAPTPERHGEPPRLMFTPPPLPPQPSQQRPPEAPWTWPEPRDSAGPPALFTPPPSDPPLQFPGREEPPLPPPPPPQPLWASEGEAETPATPPRSPLERMPWPPPLEAPGTTPELPPEFAPPAPRSPLADPHQPPAWFDDLTALVPPAHPPPRRQQQRPPPPASPLGGLGRVWPTREDLTREPAEEAPEPDPWGLEDEEGDEDVSLAQELLPPRVVESDTLINRRYMRATGLGALALLIAACRLIARRLRETRTTLKGSARRFNVDLFQVRLILG dctn1_human Dynactin subunit 1 DCTN1 150 kDa dynein-associated polypeptide DAP-150 p150-glued p135 protein protein human Homo sapiens Human MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS dctn2_human Dynactin subunit 2 Dynactin complex 50 kDa subunit 50 kDa dynein-associated polypeptide p50 dynamitin DCTN2 DCTN50 protein protein human Homo sapiens Human MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK q83419_9alph UL25 protein protein pseudorabies virus pseudorabies virus MDRAWFAFEAAAIPGSARHFIAPPFPVGFWARPGFSEGLDARLALAHANARRRAAAAALDNAMAAGARLEAEVDEQLRPLERQVERVAEALVVLEETARAAEEADAARAAEEAPEATAAADEGREVQIAKNDVALAYDANLSLDFLAMVYAARGAASGVLFGTWYATLQATLVAERPQVSRAIDSRDGRMSRTFMGVTTTALQACGRLYVGNRHYSALESAALCLHLVHRVRQGAGAAGAAAPLGIADLLERVPEYLDALSQALAEGGRISYRYNYPRVPREQLHGRYALEGHSVLAALARLRVVPGANVGANEVDGAGFVDEVNRAAAAFLGRGQNLFLGEDAPLLRATVNTITGLLLLRRLLHNGNVYGDRLRNNFQLGALVPNAPPPRGASGDAPASRSGDGNLRFLLAHYVVVAYRADERTELTQLFPGLAALALDAHSIRARVQRHQLNLVRLVALELQNRQRVTAPVNEVIAAHDAVAVQYEEGLGLLLQQPHLRNAADKRLGQFGVSSDYDLLYFLCLGYIPQFAAA vp1/2-dynactin-1 1932903 N/A 1932905 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false s6 region required to bind necessary binding region required to bind range range certain certain sequence position certain false s6-7 region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 1932906 unspecified role unspecified role prey prey physical association physical association false false false 3 vp1/2-dynein-1 1932903 N/A 1932905 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false s6 region required to bind necessary binding region required to bind range range certain certain sequence position certain false s6-7 region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 1932907 unspecified role unspecified role prey prey physical association physical association false false false 3 vp1/2-ul25-3 1932904 N/A 1932908 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1932905 unspecified role unspecified role neutral component neutral component mcherry mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 1c