HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html li-2013-7 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com swine PAM Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-8 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-9 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-10 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-11 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-12 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunostaining immunostaining Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-13 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li D., Li S., Sun Y., Dong H., Li Y., Zhao B., Guo D., Weng C., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn, wengchji@163.com Journal of virology 2013 imex curation Only protein-protein interactions imex curation f2x8a1_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNETQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYAISSTNEIGLLGAGGLTTTWKEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYARTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPETQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQSKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVISGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTRVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNAHRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV q29328_pig protein protein pig Sus scrofa (Pig) Pig MADGFSLNDALSGSGKPNPXGXPGAWGNQP c17orf72_pig Proline-rich protein 29 protein protein pig Sus scrofa (Pig) Pig similar to P0C7W0 PRR29_HUMAN f1sb42_pig EZR protein protein pig Sus scrofa (Pig) Pig MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRARFYPEDVSEELIQDITQKLFFLQVKDGILSDEIYCPPETAVLLGSYAVQAKFGDYNKELHKSGYLGSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTRKAERELSDQIQRALQLEEERKRAQEEAERLEADRVAALRAKEELERQAVDQIKSQEQLATELAEYTAKIALLEEARRRKEDEVEEWQLRVSGGGGGEDEAGSAGTEPLVDSTRAVQPPKHPPSGASFKPHGVGAASNGHNPCGKPSCHSRRLVPTTRAQGDLALPLAVRQDGRPHLRASVSCKDATEVELTVAVPEPCSFLVV frih_pig Ferritin heavy chain FTH1 FTH protein protein pig Sus scrofa (Pig) Pig MTTSCSSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHGGRGHAEKLMKLQTQRGARIFLQDIMKPERDDWENGLTAMEFALHVVKNVYQSLLELHKLATDKNDPHLCDFIETHYLHEQVKAIKELGDHITNLHRMGAPEYGMAEYLFDKHTLGSSES a0a287abg2_pig Proliferating cell nuclear antigen PCNA protein protein pig Sus scrofa (Pig) Pig MPRRCDSTTMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSPTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS i3lec2_pig PCBP1 protein protein pig Sus scrofa (Pig) Pig MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGTPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS leg1_pig Galectin-1 LGALS1 Lectin galactoside-binding soluble 1 protein protein pig Sus scrofa (Pig) Pig MACGLVASNLNLKPGECLKVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFDMHGDINTIVCNSKDGGAWGAEQRESAFPFQPGSVVEVCISFGQTDLTIKLPDGYEFSFPNRLNLEAIEHLAADGDFKIKCVAFE f2x8a1_9flav-q29328_pig-1 2846079 N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false N/A 2846087 unspecified role unspecified role prey prey physical association physical association false false false table 2 f2x8a1_9flav-c17orf72_pig-1 2846079 N/A 2846088 unspecified role unspecified role prey prey N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false physical association physical association false false false table 2 f2x8a1_9flav-ezr-1 2846079 N/A 2846089 unspecified role unspecified role prey prey N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false physical association physical association false false false table 2 f2x8a1_9flav-fth1-1 2846079 N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false N/A 2846090 unspecified role unspecified role prey prey physical association physical association false false false table 2 f2x8a1_9flav-pcna-1 2846079 N/A 2846091 unspecified role unspecified role prey prey N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false physical association physical association false false false table 2 f2x8a1_9flav-pcbp1-1 2846079 N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false N/A 2846092 unspecified role unspecified role prey prey physical association physical association false false false table 2 f2x8a1_9flav-lgals1-1 2846079 N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false N/A 2846093 unspecified role unspecified role prey prey physical association physical association false false false table 2 f2x8a1_9flav-loc100152160_pig-1 2846079 N/A 2846088 unspecified role unspecified role prey prey N/A 2846086 unspecified role unspecified role bait bait npro binding region binding-associated region binding region range range range range false physical association physical association false false false table 2 npro-pcbp1-1 2846080 N/A 2846092 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 2846086 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false npro region sufficient to bind sufficient binding region sufficient to bind range range range range false 2846132 2846129 physical association physical association false false false 2A & 3 pcbp1-npro-1 2846081 N/A 2846092 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 2846086 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false npro region sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2B npro-pcbp1-2 2846082 N/A 2846092 unspecified role unspecified role bait bait N/A 2846086 unspecified role unspecified role prey prey npro region binding region binding-associated region binding region range range range range false physical association physical association false false false 2C pcbp1-npro-2 2846083 N/A 2846092 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2846086 unspecified role unspecified role neutral component neutral component myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false npro region binding region binding-associated region binding region range range range range false colocalization colocalization false false false 2D npro-pcbp1-3 2846084 N/A 2846092 unspecified role unspecified role neutral component neutral component N/A 2846086 unspecified role unspecified role neutral component neutral component npro region binding region binding-associated region binding region range range range range false colocalization colocalization false false false 2E npro-pcbp1-5 2846085 N/A 2846092 unspecified role unspecified role prey prey N/A 2846086 unspecified role unspecified role bait bait npro region binding region binding-associated region binding region range range range range false physical association physical association false false false 1