HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html rosnoblet-2012-1 Hepatitis C virus NS5B and host cyclophilin A share a common binding site on NS5A. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Rosnoblet C., Fritzinger B., Legrand D., Launay H., Wieruszeski J.M., Lippens G., Hanoulle X. Virus - Publications including interactions involving viral proteins xavier.hanoulle@univ-lille1.fr. xavier.hanoulle@univ-lille1.fr J. Biol. Chem. (0021-9258) 2012 imex curation Only protein-protein interactions imex curation rosnoblet-2012-2 Hepatitis C virus NS5B and host cyclophilin A share a common binding site on NS5A. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Rosnoblet C., Fritzinger B., Legrand D., Launay H., Wieruszeski J.M., Lippens G., Hanoulle X. Virus - Publications including interactions involving viral proteins xavier.hanoulle@univ-lille1.fr. xavier.hanoulle@univ-lille1.fr J. Biol. Chem. (0021-9258) 2012 imex curation Only protein-protein interactions imex curation q99ib8-pro_0000045603 RNA-directed RNA polymerase protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SMSYSWTGALITPCSPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRAKKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLEEACQLTPPHSARSKYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDPAKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQRVEYLLKAWAEKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPEEARTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKALAACKAAGIVAPTMLVCGDDLVVISESQGTEEDERNLRAFTEAMTRYSAPPGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWETVRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLNFEMYGSVYSVNPLDLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAVRASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEARLLDLSSWFTVGAGGGDIFHSVSRARPRSLLFGLLLLFVGVGLFLLPAR 2443 3033 q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 ppia_human Peptidyl-prolyl cis-trans isomerase A Cyclophilin A Cyclosporin A-binding protein PPIA CYPA Rotamase A protein protein human Homo sapiens Human MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE ns5a-ns5b-4 1445481 n/a NS5B 1445483 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a NS5A 1445484 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1A and 1C. Supp. data 2 and 5 ns5a-cypa-1 1445481 n/a NS5A 1445484 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gly304-pro323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gly337-pro341 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a CypA 1445485 unspecified role unspecified role unspecified role unspecified role ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1D ns5a-ns5b-5 1445481 n/a NS5A 1445484 unspecified role unspecified role unspecified role unspecified role 2h label 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a NS5B 1445483 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1445507 1445504 direct interaction direct interaction false false false Fig. 5A and 5B ns5a-ns5b-2 1445482 n/a NS5A 1445484 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a NS5B 1445483 unspecified role unspecified role unspecified role unspecified role region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1445512 1445510 direct interaction direct interaction false false false 1445482 Fig. 2