IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ helbig-2011-2 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Helbig KJ., Eyre NS., Yip E., Narayana S., Li K., Fiches G., McCartney EM., Jangra RK., Lemon SM., Beard MR. Virus - Publications including interactions involving viral proteins According to Ref14, in fig 2D the NNeo3-5B cells contains the sequence of a HCV replicon with GenBank accession AF139594. (UniProt Q9WPH5) michael.beard@adelaide.edu.au Hepatology (Baltimore, Md.) 2011 imex curation Only protein-protein interactions helbig-2011-3 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret antibody detection identification by antibody antibody detection Helbig KJ., Eyre NS., Yip E., Narayana S., Li K., Fiches G., McCartney EM., Jangra RK., Lemon SM., Beard MR. Virus - Publications including interactions involving viral proteins Secondary antibodies were tagged with fluorophores- rather than the directly interacting molecules . michael.beard@adelaide.edu.au Hepatology (Baltimore, Md.) 2011 imex curation Only protein-protein interactions helbig-2011-4 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Helbig KJ., Eyre NS., Yip E., Narayana S., Li K., Fiches G., McCartney EM., Jangra RK., Lemon SM., Beard MR. Virus - Publications including interactions involving viral proteins NNeoC-5B cells were used: according to Ref 14 these harbor an HCV replicon with the complete HCV genome, GenBank AF139594 (UniProt Q9WPH5). michael.beard@adelaide.edu.au Hepatology (Baltimore, Md.) 2011 imex curation Only protein-protein interactions q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 rsad2_human Radical S-adenosyl methionine domain-containing protein 2 RSAD2 CIG5 Cytomegalovirus-induced gene 5 protein Viperin Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible protein protein human Homo sapiens Human MWVLTPAAFAGKLLSVFRQPLSSLWRSLVPLFCWLRATFWLLATKRRKQQLVLRGPDETKEEEEDPPLPTTPTSVNYHFTRQCNYKCGFCFHTAKTSFVLPLEEAKRGLLLLKEAGMEKINFSGGEPFLQDRGEYLGKLVRFCKVELRLPSVSIVSNGSLIRERWFQNYGEYLDILAISCDSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW q9wph5_9hepc Core protein precursor Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein Gp32 Hepacivirin NS1 NS3P NS5B Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase gp35 gp68 gp70 p21 p27 p68 p8 Mature core protein NS3 helicase NS3 protease Protease NS2 Serine protease/helicase NS3 Viroporin p7 Viroporin p70 p23 p56/58 protein protein hepatitis c virus subtype 1b Hepatitis C virus subtype 1b MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRAIRKTSERSQPRGRRQPIPKARRPEGRAWAQPGYPWPLYGNEGMGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGLADLMGYIPLVGGPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTVPASAHEVRNASGVYHVTNDCSNSSIVFEAADLIMHTPGCVPCVREGNSSRCWVALTPTLAARNATIPTTTIRHHVDLLVGAAALCSAMYVGDLCGSVFLVSQLFTFSPRRHATLQDCNCSIYPGHASGHRMAWDMMMNWSPTTALVVSQLLRIPQAVIDMVAGAHWGVLAGLAYYSMAGNWAKVLIVMLLFAGVDGHTLTTGGHAARLTSGFAGLFTPGPSQRIQLINTNGSWHINRTALNCNDSLQTGFLAALFYAHRFNSSGCPERMASCRSIDKFDQGWGPITYAEPTKDPDQRPYCWHYPPQQCGIVPASQVCGPVYCFTPSPVVVGTTDRLGNPTYSWGENDTDVLLLNNTRPPQGNWFGCTWMNSTGFTKTCGAPPCNIGGVGNNTLTCPTDCFRKHPEATYSKCGSGPWLTPRCMVDYPYRLWHYPCTVNFSIFKVRMYVGGVEHRLNAACNWTRGERCNLDDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGIGSAVVSFAIKWEYVVLLFLLLADARVCACLWMMLLIAQAEAALENLVALNAASVAGAHGILSFLVFFCAAWYIKGRLVPGAAYAFYGAWPLLLLLLTLPPRAYAMDREMAASCGGAVFVGLALLTLSPYYKVFLARLLWWLQYLITRAEAHLHVWVPPLNVRGGRDAIILLTCAVHPELIFDITKLLIAILGPLMVLQAGITRVPYFVRAQGLIRACMLVRKVAGGHYVQMAFMRLGALTGTYVYNHLTPLRDWAHAGLRDLAVAVEPVVFSDMETKIITWGADTAACGDIILGLPVSARRGREILLGPADSLVGQGWRLLAPITAYSQQTRGLLGCIITSLTGRDKNQVEGEVQVVSTATQSFLATCVNGVCWTVYHGAGSKTLAGPKGPIAQMYTNVDQDLVGWPAPPGARSLTPCTCGSSDLYLVTRHADVIPVRRRGDNRGSLLSPRPVSYLKGSSGGPLLCPSGHAVGVFRAAVCTRGVAKAVDFVPVESMETTMRSPVFTDNSSPPAVPQTFQVAHLHAPTGSGKSTRVPAAYAAQGYKVLVLNPSVAATLGFGAYMSKAHGTDPNIRTGVRTITTGAPITYSTYGKFLADGGCSGGAYDIIMCDECHSTDSTTILGIGTVLDQAETAGARLVVLATATPPGSVTVPHPNIEEVALSNTGEIPFYGKAIPIEAIKGGRHLIFCHSKKKCDELAAKLSGLGINAVAYYRGLDVSVIPTSGDVVVVATDALMTGYTGDFDSVIDCNTCVTQTVDFSLDPTFTIETTTVPQDAVSRSQRRGRTGRGRGGIYRFVTPGERPSGMFDSSVLCECYDAGCAWYELTPAETSVRLRAYLNTPGLPVCQDHLEFWESVFTGLTHIDAHFLSQTKQAGDNFPYLVAYQATVCARAQAPPPSWDQMWKCLIRLKPTLHGPTPLLYRLGAVQNEVTLTHPITKYIMACMSADLEVVTSTWVLVGGVLAALAAYCLTTGSVVIVGRIILSGRPAVVPDREVLYREFDEMEECASHLPYIEQGMQLAEQFKQKALGLLQTATKQAEAAAPVVESKWRALETFWAKHMWNFISGIQYLAGLSTLPGNPAIASLMAFTASITSPLTTQNTLLFNILGGWVAAQLAPPSAASAFVGAGIAGAAVGSIGLGKVLVDILAGYGAGVAGALVAFKVMSGEAPSAEDLINLLPAILSPGALVVGVVCAAILRRHVGPGEGAVQWMNRLIAFASRGNHVSPTHYVPESDAAARVTQVLSSLTITQLLKRLHQWINEDCSTPCSGSWLRDVWDWVCTVLSDFKTWLQSKLLPRLPGVPFLSCQRGYKGVWRGDGIMHTTCPCGAQIAGHVKNGSMRIIGPKTCSNTWHGTFPINAYTTGPCTPSPAPNYSKALWRVAAEEYVEVTRVGDFHYVTGITTDNVKCPCQVPAPEFFTEVDGVRLHRYAPVCKPLLRDEVVFQVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAEAAKRRLARGSPPSLASSSASQLSAPSLRATCTTHSSYNLDSPDVDLIEANLLWRQEMGGNITRVESENKVVVLDSFEPLRAEGDENEISIAAEILRKSKKFPAAIPIWARPDYNPPLLESWKNPDYVPPVVHGCPLPPVKAPPIPPPRRKRTVVLTDSTVSSVLAELATKTFGSSELSAADSGTATAPPDQTSDNGGKDSDAESCSSMPPLEGEPGDPDLSDGSWSTVSEEAGESVVCCSMSYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSAGLRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSIEEACRLTPPHSAKSKFGYGAKDVRNLSSRAINHIRSVWEDLLEDTVTPIDTTVMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSTLPQAVMGSSYGFQYSPGQRVEFLVKAWKSKKNPMGFSYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIKSLTERLYIGGPLTNSKGQSCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQDCTMLVNGDDLVVICESAGTQEDAASLRVFTEAMTRYSAPPGDLPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPIARAAWETARHTPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRAKLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASQLDLSGWFVAGYSGGDIYHSLSRARPRWFMLCLLLLSVGVGIYLLPNR q99ib8-pro_0000045593 Mature core protein protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV STNPKPQRKTKRNTNRRPEDVKFPGGGQIVGGVYLLPRRGPRLGVRTTRKTSERSQPRGRRQPIPKDRRSTGKAWGKPGRPWPLYGNEGLGWAGWLLSPRGSRPSWGPTDPRHRSRNVGKVIDTLTCGFADLMGYIPVVGAPLSGAARAVAHGVRVLEDGVNYATGNLPGFPFSIF 2 177 vapa_human Vesicle-associated membrane protein-associated protein A VAPA VAP33 33 kDa VAMP-associated protein protein protein human Homo sapiens Human MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL 234qwrawer-1 2871557 N/A 2871560 unspecified role unspecified role neutral component neutral component N/A 2871561 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1D, 2 A/B q99ib8_pro_0000045602-rsad2-1 2871557 N/A 2871562 unspecified role unspecified role neutral component neutral component NS5A_protein sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 2871561 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2C q99ib8_pro_0000045602-rsad2-2 2871557 N/A 2871563 unspecified role unspecified role neutral component neutral component N/A 2871561 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2A rsad2-vapa-1 2871557 N/A 2871564 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2871561 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 7A core-rsad2-1 2871558 N/A 2871563 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A 2871561 unspecified role unspecified role fluorescence donor fluorescence donor proximity proximity close-contact colocalization neighbourhood interaction false false false 3B q99ib8_pro_0000045602-rsad2-3 2871558 N/A 2871560 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A 2871561 unspecified role unspecified role fluorescence donor fluorescence donor proximity proximity close-contact colocalization neighbourhood interaction false false false 3C rsad2-pro_0000045593-1 2871558 N/A 2871563 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A 2871561 unspecified role unspecified role fluorescence donor fluorescence donor c_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 5B rsad2-q99ib8_pro_0000045602-1 2871558 N/A 2871560 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A 2871561 unspecified role unspecified role fluorescence donor fluorescence donor c_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 5A rsad2-q9wph5_9hepc-1 2871559 N/A 2871564 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2871561 unspecified role unspecified role fluorescence donor fluorescence donor region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false 7B