HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html gladue-2011-1 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov Swine primary macrophage Virology 2011 imex curation Only protein-protein interactions imex curation gladue-2011-2 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov Swine primary macrophage Virology 2011 imex curation Only protein-protein interactions imex curation k9iwf9_pig IQGAP1 protein protein pig Sus scrofa (Pig) Pig MSATDEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMGEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKIELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDHRIPADTFTALKNPNAMLVNLEEPLASTYQDVLYQAKQDKMTNAKNRTESSERERDVYEELLTQAEIQGNINKVNMIAALANVDQALEQGSALALFKILQSPALGLRGLQQQNSDWYLKQLLSDRQQKRESGQAEPLQKEELQSGVDAGNIAAQQYQRRLAAVAAINAAIQKGVAEKTVLELMNPEAQLPQVYPFAAELYQKELATLQQQSPEHSLTHPELSVAVEMLSSVALINRALESGDMNTVWRQLSSSVTGLTNIEEENYQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQTPAAKLEGVLAEVAQHYQDTLIRAKREKAQETQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVDSGDVSRTLGALRSPDVGLYGVIPECGQTYQSDLAEAKKKKLAAGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPDFVQNFMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQACCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVIKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMIVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMLNKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVRTRLDNSIRNMRAVTDKFLAAIISSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGEGSGNVNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQSGLKKLTELGTVDPKNRYQELINDIAKDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK p21530-pro_0000038063 Capsid protein C protein protein csfvb Classical swine fever virus (strain Brescia) Hog cholera virus SDDGASASKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVAA 169 267 core-iqgap1-1 2846309 N/A 2846311 unspecified role unspecified role prey prey N/A 2846312 unspecified role unspecified role bait bait P21530-PRO_0000038063:p.Val42_Glu44delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P21530-PRO_0000038063:p.Gly64_Asn68delinsAlaAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P21530-PRO_0000038063:p.Thr40_Ile41delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P21530-PRO_0000038063:p.Gly45_Lys47delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 & table 1 core-iqgap1-2 2846310 N/A 2846311 unspecified role unspecified role prey prey N/A 2846312 unspecified role unspecified role bait bait physical association physical association false false false results The IQGAP1 recovered from the library contained amino acids (502–704) of homo sapiens IQGAP1 (NCBI Reference Sequence: AAI39732) amino terminal fused to the GAL4-AD.