HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html chen-2010d-2 Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw J. Virol. (0022-538X) 2010 imex curation Only protein-protein interactions imex curation chen-2010d-1 Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw J. Virol. (0022-538X) 2010 imex curation Only protein-protein interactions imex curation chen-2010d-3 Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw J. Virol. (0022-538X) 2010 imex curation Only protein-protein interactions imex curation q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS ns5a-plk1-1 1455165 n/a NS5A 1455168 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a PLK1 1455169 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4B ns5a-plk1-2 1455165 n/a PLK1 1455169 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a NS5A 1455168 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4C ns5a-plk1-3 1455166 n/a NS5A 1455168 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a PLK1 1455169 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A plk1-ns5a-1 1455167 n/a PLK1 1455169 unspecified role unspecified role neutral component neutral component n/a NS5A 1455168 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4D