HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html zahoor-2010-3 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation zahoor-2010-1 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp Bovine Madin–Darby bovine kidney (MDBK) cDNA J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation zahoor-2010-2 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation zahoor-2010-4 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation zahoor-2010-5 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation zahoor-2010-6 Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K confocal microscopy confocal microscopy immunostaining immunostaining Zahoor M.A., Yamane D., Mohamed Y.M., Suda Y., Kobayashi K., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins akashih@mail.ecc.u-tokyo.ac.jp J. Gen. Virol. (0022-1317) 2010 imex curation Only protein-protein interactions imex curation tppc9_bovin Trafficking protein particle complex subunit 9 TRAPPC9 NIBP NIK- and IKBKB-binding protein protein protein bovin Bos taurus (Bovine) Bovine MSVPDYMQCAEDHQTLLVVVQPVGIVSEENFFRIYKRISSVSQISVRDSQRALYIRYRHHYPPENSEWGDFQTHRKVVGLITITDCFSAKDWPQIFEKFHVQKEIYGSTLYDSRLFVFGLQGEIAEQPRTDVAFYPSYEDCATVEKRIEDFVESLFIVLESKRLDRATDKSGDKIPLLCVPFEKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGMLQDSLVHYHMSVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTGGKAGARRFPGSALPAEAANRHRPGALTTNGINADTSTEIGRAKNCLSPEDIIEKYKEAISYYSKYKNAGVIELEACVKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILSELYELIGFHRKSAFFKRVAAMQCVAPSISEPGWRACYKLLLETLPGYSLSLDPQDFNKGTHRGWAAVQMRLLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVTQSLENYTSKCPGTMELLTLPDGLTLPPVPFTKLPIVRRVKLLDLPASLRPQKMKSSLGPSVSAKSPFIYSPIIAHSRGEERSKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVSGYHTTVFGVFSDCLLDSLPGIKTSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDKISTNVSVQLYNGETQQLVVRLENIGMEPLEKLEVTSKILTTKEKLYGDFLSWKLEDTLAQFPLQPGKVATFTISIKVKLDFSCQENLLQDLSDDGISVSGFPLCSPFRQVVRPRVESKPVNAAEGGRPGEPCHVKTLEAVLNFKYSGGPGHVEGYYRNLALGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNATEHELSVGARSDEELILHAGECQRMAIQVDKFNFESFPESPADKGQFANSKHLEEERQEARGLEINSKLDIHWTIPSLKRTGEASVEGLLNQLVLEHLQLAPLLWDVLVDGQPCDCQAAACRVGDPVRLEVRLTNRSPRSVGPFALSVVPFQDHQNGVHSYDLRHAVSFVGSGTFYLDKVQPSGQAACLGALLFLYTGDFFLHIRFHEDGPSKELPPSWFCLPSVHVRALEAQA p19711-pro_0000038035 Non-structural protein 5A protein protein bvdvn Bovine viral diarrhea virus (isolate NADL) SGNYILDLIYGLHKQINRGLKKMVLGWAPAPFSCDWTPSDERIRLPTDNYLRVETRCPCGYEMKAFKNVGGKLTKVEESGPFLCRNRPGRGPVNYRVTKYYDDNLREIKPVAKLEGQVEHYYKGVTAKIDYSKGKMLLATDKWEVEHGVITRLAKRYTGVGFNGAYLGDEPNHRALVERDCATITKNTVQFLKMKKGCAFTYDLTISNLTRLIELVHRNNLEEKEIPTATVTTWLAYTFVNEDVGTIKPVLGERVIPDPVVDINLQPEVQVDTSEVGITIIGRETLMTTGVTPVLEKVEPDASDNQNSVKIGLDEGNYPGPGIQTHTLTEEIHNRDARPFIMILGSRNSISNRAKTARNINLYTGNDPREIRDLMAAGRMLVVALRDVDPELSEMVDFKGTFLDREALEALSLGQPKPKQVTKEAVRNLIEQKKDVEIPNWFASDDPVFLEVALKNDKYYLVGDVGELKDQAKALGATDQTRIIKEVGSRTYAMKL 2774 3269 ns5a-nibp-2 1538310 n/a 1538316 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1538317 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 2A ns5a-trappc9-2 1538310 n/a 1538316 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1538317 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 2A ns5a-nibp-5 1538311 n/a 1538317 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1538316 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false No figure ns5a-nibp-1 1538312 n/a 1538317 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1538316 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 ns5a-trappc9-1 1538313 n/a 1538317 unspecified role unspecified role prey prey n/a 1538316 unspecified role unspecified role bait bait physical association physical association false false false 2B ns5a-nibp-3 1538314 n/a 1538316 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1538317 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3C ns5a-nibp-4 1538315 n/a 1538316 unspecified role unspecified role neutral component neutral component ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1538317 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4A