HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html yamane-2009-2 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation yamane-2009-3 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation yamane-2009-4 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. bovin-mdbk Bos taurus mdbk Madin-Darby bovine kidney cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation yamane-2009-5 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. bovin-lb9.k Bos taurus lb9.k Bovine sarcoma cell line LB9.K fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation yamane-2009-1 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp MDBK cDNA J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation yamane-2009-6 Inhibition of sphingosine kinase by bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yamane D., Zahoor M.A., Mohamed Y.M., Azab W., Kato K., Tohya Y., Akashi H. Virus - Publications including interactions involving viral proteins sequence length of XP_876032 (392 aa) is not the same as in the paper (489 aa) akashih@mail.ecc.u-tokyo.ac.jp J. Biol. Chem. (0021-9258) 2009 imex curation Only protein-protein interactions imex curation sphk1_bovine_protein sphingosine kinase 1 isoform X6 protein protein bovin Bos taurus (Bovine) Bovine p19711-pro_0000038032 Serine protease NS3 protein protein bvdvn Bovine viral diarrhea virus (isolate NADL) GPAVCKKITEHEKCHINILDKLTAFFGIMPRGTTPRAPVRFPTSLLKVRRGLETAWAYTHQGGISSVDHVTAGKDLLVCDSMGRTRVVCQSNNRLTDETEYGVKTDSGCPDGARCYVLNPEAVNISGSKGAVVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEESKPTKIMSGIQTVSKNRADLTEMVKKITSMNRGDFKQITLATGAGKTTELPKAVIEEIGRHKRVLVLIPLRAAAESVYQYMRLKHPSISFNLRIGDMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSYIFLDEYHCATPEQLAIIGKIHRFSESIRVVAMTATPAGSVTTTGQKHPIEEFIAPEVMKGEDLGSQFLDIAGLKIPVDEMKGNMLVFVPTRNMAVEVAKKLKAKGYNSGYYYSGEDPANLRVVTSQSPYVIVATNAIESGVTLPDLDTVIDTGLKCEKRVRVSSKIPFIVTGLKRMAVTVGEQAQRRGRVGRVKPGRYYRSQETATGSKDYHYDLLQAQRYGIEDGINVTKSFREMNYDWSLYEEDSLLITQLEILNNLLISEDLPAAVKNIMARTDHPEPIQLAYNSYEVQVPVLFPKIRNGEVTDTYENYSFLNARKLGEDVPVYIYATEDEDLAVDLLGLDWPDPGNQQVVETGKALKQVTGL 1680 2362 p19711-pro_0000038030 Non-structural protein 2-3 protein protein bvdvn Bovine viral diarrhea virus (isolate NADL) DSGGQEYLGKIDLCFTTVVLIVIGLIIARRDPTIVPLVTIMAALRVTELTHQPGVDIAVAVMTITLLMVSYVTDYFRYKKWLQCILSLVSAVFLIRSLIYLGRIEMPEVTIPNWRPLTLILLYLISTTIVTRWKVDVAGLLLQCVPILLLVTTLWADFLTLILILPTYELVKLYYLKTVRTDTERSWLGGIDYTRVDSIYDVDESGEGVYLFPSRQKAQGNFSILLPLIKATLISCVSSKWQLIYMSYLTLDFMYYMHRKVIEEISGGTNIISRLVAALIELNWSMEEEESKGLKKFYLLSGRLRNLIIKHKVRNETVASWYGEEEVYGMPKIMTIIKASTLSKSRHCIICTVCEGREWKGGTCPKCGRHGKPITCGMSLADFEERHYKRIFIREGNFEGMCSRCQGKHRRFEMDREPKSARYCAECNRLHPAEEGDFWAESSMLGLKITYFALMDGKVYDITEWAGCQRVGISPDTHRVPCHISFGSRMPFRQEYNGFVQYTARGQLFLRNLPVLATKVKMLMVGNLGEEIGNLEHLGWILRGPAVCKKITEHEKCHINILDKLTAFFGIMPRGTTPRAPVRFPTSLLKVRRGLETAWAYTHQGGISSVDHVTAGKDLLVCDSMGRTRVVCQSNNRLTDETEYGVKTDSGCPDGARCYVLNPEAVNISGSKGAVVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEESKPTKIMSGIQTVSKNRADLTEMVKKITSMNRGDFKQITLATGAGKTTELPKAVIEEIGRHKRVLVLIPLRAAAESVYQYMRLKHPSISFNLRIGDMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSYIFLDEYHCATPEQLAIIGKIHRFSESIRVVAMTATPAGSVTTTGQKHPIEEFIAPEVMKGEDLGSQFLDIAGLKIPVDEMKGNMLVFVPTRNMAVEVAKKLKAKGYNSGYYYSGEDPANLRVVTSQSPYVIVATNAIESGVTLPDLDTVIDTGLKCEKRVRVSSKIPFIVTGLKRMAVTVGEQAQRRGRVGRVKPGRYYRSQETATGSKDYHYDLLQAQRYGIEDGINVTKSFREMNYDWSLYEEDSLLITQLEILNNLLISEDLPAAVKNIMARTDHPEPIQLAYNSYEVQVPVLFPKIRNGEVTDTYENYSFLNARKLGEDVPVYIYATEDEDLAVDLLGLDWPDPGNQQVVETGKALKQVTGL 1137 2362 ns3-sphk1-1 1540102 n/a 1540108 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1540109 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 1D and 4 ns2-3-sphk1-1 1540102 n/a 1540108 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1540110 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 1D ns3-sphk1-5 1540102 n/a 1540108 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1540109 unspecified role unspecified role prey prey n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3 ns2-3-sphk1-2 1540103 n/a 1540108 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1540110 unspecified role unspecified role bait bait physical association physical association false false false 1E ns3-sphk1-2 1540103 n/a 1540108 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1540109 unspecified role unspecified role bait bait physical association physical association false false false 1E ns3-sphk1-3 1540104 n/a 1540108 unspecified role unspecified role prey prey n/a 1540109 unspecified role unspecified role bait bait physical association physical association false false false 1F ns2-3-sphk1-3 1540104 n/a 1540108 unspecified role unspecified role prey prey n/a 1540110 unspecified role unspecified role bait bait physical association physical association false false false 1F ns2-3-sphk1-4 1540105 n/a 1540108 unspecified role unspecified role neutral component neutral component n/a 1540110 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2A ns3-sphk1-4 1540105 n/a 1540108 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1540109 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2A ns3-sphk1-7 1540106 n/a 1540108 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1540109 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1540153 1540151 physical association physical association false false false No figure ns3-sphk1-6 1540107 n/a 1540108 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1540109 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 5B