IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ miknis-2009-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Journal of virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus miknis-2009-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Journal of virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus miknis-2009-4 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Journal of virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus p0c6u8-pro_0000338263 Non-structural protein 9 ORF1a polyprotein 1a protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus NNELSPVALRQMSCAAGTTQTACTDDNALAYYNNSKGGRFVLALLSDHQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ 4118 4230 pro_0000338263-1 3712672 N/A 3712675 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0C6U8-PRO_0000338263:p.Gly104Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 2.6Å Rwork: 0.216 Rfree: 0.268 pro_0000338263-2 3712673 N/A 3712675 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0C6U8-PRO_0000338263:p.Gly104Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6U8-PRO_0000338263:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6U8-PRO_0000338263:p.Gly104Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 2 Dimerization occurs at protein concentrations between 40 and 105 M // A weak dimerization constant of 0.871 mM was determined for G104V pro_0000338263-4 3712674 N/A 3712675 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0C6U8-PRO_0000338263:p.Gly104Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6U8-PRO_0000338263:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6U8-PRO_0000338263:p.Gly104Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 2 Dimerization occurs at protein concentrations between 40 and 105 M // A weak dimerization constant of 0.871 mM was determined for G104V