HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html okamoto-2008-2 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation okamoto-2008-5 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation okamoto-2008-3 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation okamoto-2008-4 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation okamoto-2008-6 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation okamoto-2008-7 A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp J. Virol. (0022-538X) 2008 imex curation Only protein-protein interactions imex curation q9wmx2-pro_0000037551 Non-structural protein 5A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYKGVWRGDGIMQTTCPCGAQITGHVKNGSMRIVGPRTCSNTWHGTFPINAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFLVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFEPLQAEEDEREVSVPAEILRRSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLSESTVSSALAELATKTFGSSESSAVDSGTATASPDQPSDDGDAGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCC 1973 2419 fkbp8_human Peptidyl-prolyl cis-trans isomerase FKBP8 FK506-binding protein 8 38 kDa FK506-binding protein FKBPR38 FKBP8 FKBP38 Rotamase protein protein human Homo sapiens Human MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN ns5a-fkbp8-12 1477636 n/a 1477642 unspecified role unspecified role bait bait c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1477643 unspecified role unspecified role prey prey n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1B and 1C fkbp8-ns5a-14 1477636 n/a 1477642 unspecified role unspecified role prey prey c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1477643 unspecified role unspecified role bait bait n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1B and 1D ns5a-fkbp8-10 1477637 n/a 1477643 unspecified role unspecified role neutral component neutral component n/a 1477642 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection Q9WMX2-PRO_0000037551:p.Val121Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 3 fkbp8-ns5a-10 1477637 n/a 1477643 unspecified role unspecified role neutral component neutral component n/a 1477642 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 6C Cells contain an HCV subgenomic replicon ns5a-fkbp8-13 1477638 n/a 1477642 unspecified role unspecified role bait bait c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1477643 unspecified role unspecified role prey prey n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1477638 Fig. 1E ns5a-fkbp8-9 1477639 n/a 1477643 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1477642 unspecified role unspecified role bait bait Q9WMX2-PRO_0000037551:p.Val121Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2B and 2C fkbp8-ns5a-11 1477640 n/a 1477643 unspecified role unspecified role neutral component neutral component n/a 1477642 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7A fkbp8-ns5a-12 1477641 n/a 1477642 unspecified role unspecified role neutral component neutral component n/a 1477643 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7A