HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html sklan-2007-2 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins jeffrey.glenn@stanford.edu J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation sklan-2007-6 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins jeffrey.glenn@stanford.edu J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation sklan-2007-1 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins jeffrey.glenn@stanford.edu Human liver cDNA J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation sklan-2007-3 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins Cross-linking was performed using Dithiobis(succinimidylpropionate) followed by immunoprecipitation. jeffrey.glenn@stanford.edu J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation sklan-2007-5 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins jeffrey.glenn@stanford.edu J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation sklan-2007-7 A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Sklan E.H., Staschke K., Oakes T.M., Elazar M., Winters M., Aroeti B., Danieli T., Glenn J.S. Virus - Publications including interactions involving viral proteins jeffrey.glenn@stanford.edu J. Virol. (0022-538X) 2007 Only protein-protein interactions imex curation p27958-pro_0000037576 Non-structural protein 5A protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) SGSWLRDIWDWICEVLSDFKTWLKAKLMPQLPGIPFVSCQRGYRGVWRGDGIMHTRCHCGAEITGHVKNGTMRIVGPRTCKNMWSGTFFINAYTTGPCTPLPAPNYKFALWRVSAEEYVEIRRVGDFHYVSGMTTDNLKCPCQIPSPEFFTELDGVRLHRFAPPCKPLLREEVSFRVGLHEYPVGSQLPCEPEPDVAVLTSMLTDPSHITAEAAGRRLARGSPPSMASSSASQLSAPSLKATCTANHDSPDAELIEANLLWRQEMGGNITRVESENKVVILDSFDPLVAEEDEREVSVPAEILRKSRRFAPALPVWARPDYNPLLVETWKKPDYEPPVVHGCPLPPPRSPPVPPPRKKRTVVLTESTLPTALAELATKSFGSSSTSGITGDNTTTSSEPAPSGCPPDSDVESYSSMPPLEGEPGDPDLSDGSWSTVSSGADTEDVVCC 1973 2420 tbc20_human TBC1 domain family member 20 TBC1D20 C20orf140 protein protein human Homo sapiens Human MALRSAQGDGPTSGHWDGGAEKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRRKVWPKLLNVNANDPPPISGKNLRQMSKDYQQVLLDVRRSLRRFPPGMPEEQREGLQEELIDIILLILERNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLSDFRHVVRLYDFFLACHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQQAERTAASTFKDFELASAQQRPDMVLRQRFRGLLRPEDRTKDVLTKPRTNRFVKLAVMGLTVALGAAALAVVKSALEWAPKFQLQLFP ns5a-tbc1d20-3 1511857 n/a 1511863 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1511864 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1B ns5a-tbc1d20 1511857 n/a 1511864 unspecified role unspecified role prey prey n/a 1511863 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A tbc1d20-ns5a 1511857 n/a 1511864 unspecified role unspecified role bait bait n/a 1511863 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A tbc1d20-ns5a-1 1511858 n/a 1511864 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1511863 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2B tbc1d20_ns5a 1511858 n/a 1511863 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1511864 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig. 2C ns5a-tbc1d20-2 1511859 n/a 1511863 unspecified role unspecified role bait bait n/a 1511864 unspecified role unspecified role prey prey physical association physical association false false false No figure ns5a-tbc1d20-1 1511860 n/a 1511863 unspecified role unspecified role neutral component neutral component n/a 1511864 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1C tbc1d20-ns5a 1511861 n/a 1511864 unspecified role unspecified role bait bait n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro n/a 1511863 unspecified role unspecified role prey prey P27958-PRO_0000037576:p.[Ile8Asp;Ile12Glu;Phe19Asp] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 1D tbc1d20-ns5a-2 1511862 n/a 1511864 unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1511863 unspecified role unspecified role neutral component neutral component c-terminal rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig. 2