HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html kim-2007m-3 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-2 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-5 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Human thymus cDNA J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-1 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-4 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-6 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Human thymus cDNA J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation kim-2007m-7 An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr J. Virol. (0022-538X) 2007 imex curation Only protein-protein interactions imex curation p27958-pro_0000037577 RNA-directed RNA polymerase protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) SMSYSWTGALVTPCAAEEQKLPINALSNSLLRHHNLVYSTTSRSACQRKKKVTFDRLQVLDSHYQDVLKEVKAAASKVKANLLSVEEACSLAPPHSAKSKFGYGAKDVRCHARKAVAHINSVWKDLLEDSVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSKLPLAVMGSSYGFQYSPGQRVEFLVQAWKSKKTPMGLSYDTRCFDSTVTESDIRTEEAIYQCCDLDPQARVAIKSLTERLYVGGPLTNSRGENCGYRRCRASRVLTTSCGNTLTRYIKARAACRAAGLQDCTMLVCGDDLVVICESAGVQEDAASLRAFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDGAGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMFAPTLWARMILMTHFFSVLIARDQLEQALNCEIYGACYSIEPLDLPPIIQRLHGLSAFSLHSYSPGEINRVAACLRKLGVPPLRAWRHRAWSVRARLLARGGKAAICGKYLFNWAVRTKLKLTPITAAGRLDLSGWFTAGYSGGDIYHSVSHARPRWFWFCLLLLAAGVGIYLLPNR 2421 3011 sept6_human Septin-6 SEPTIN6 KIAA0128 SEP2 SEPT6 protein protein human Homo sapiens Human MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE roa1_human Heterogeneous nuclear ribonucleoprotein A1 HNRNPA1 HNRPA1 Helix-destabilizing protein Single-strand RNA-binding protein hnRNP core protein A1 protein protein human Homo sapiens Human MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF sept2_human Septin-2 SEPTIN2 DIFF6 KIAA0158 NEDD5 Neural precursor cell expressed developmentally down-regulated protein 5 SEPT2 protein protein human Homo sapiens Human MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV 12CCBBE30806F92F if4a2_human Eukaryotic initiation factor 4A-II ATP-dependent RNA helicase eIF4A-2 EIF4A2 DDX2B EIF4F protein protein human Homo sapiens Human MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN rl6_human 60S ribosomal protein L6 RPL6 TXREB1 Tax-responsive enhancer element-binding protein 107 Neoplasm-related protein C140 Large ribosomal subunit protein eL6 protein protein human Homo sapiens Human MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF hnrnpa1-septin6-ns5b-septin2-1 1501052 n/a 1501059 unspecified role unspecified role prey prey n/a 1501060 unspecified role unspecified role bait bait n/a 1501061 unspecified role unspecified role prey prey n/a 1501062 unspecified role unspecified role prey prey association association false false false Fig. 3D hnrnpa1-ns5b-1 1501052 n/a 1501059 unspecified role unspecified role prey prey n/a 1501061 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1E sept6-hnrnpa1-septin2-1 1501052 n/a 1501061 unspecified role unspecified role prey prey n/a 1501060 unspecified role unspecified role bait bait n/a 1501062 unspecified role unspecified role prey prey association association false false false Fig. 3D ns5b-hnrnpa1-1 1501053 n/a 1501061 unspecified role unspecified role prey prey n/a 1501059 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1B and D hnrnpa1-septin6-1 1501053 n/a 1501061 unspecified role unspecified role prey prey n/a 1501060 unspecified role unspecified role bait bait egfp egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3Cii ns5b-hnrnpa1-2 1501054 n/a 1501059 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1501060 unspecified role unspecified role prey prey physical association physical association false false false Results ns5a-if4a2-1 1501054 n/a 1501063 unspecified role unspecified role prey prey n/a 1501059 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Results ns5b-multi-2 1501055 n/a 1501061 unspecified role unspecified role prey prey n/a 1501064 unspecified role unspecified role prey prey n/a 1501065 unspecified role unspecified role prey prey n/a 1501059 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false 1A and C p27958_pro-hnrnpa1-1 1501056 n/a 1501059 unspecified role unspecified role neutral component neutral component n/a 1501061 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2 ns5b-hnrnpa1-3 1501057 n/a 1501060 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1501059 unspecified role unspecified role bait bait physical association physical association false false false 3B ns5b-hnrnpa1-4 1501058 n/a 1501059 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1501060 unspecified role unspecified role prey prey egfp egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3Bii