DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ luo-2005-7 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr experimental particp experimental participant identification experimental particp Luo H., Wu D., Shen C., Chen K., Shen X., Jiang H. Coronavirus - Interactions investigated in the context of Coronavirus xshen@mail.shcnc.ac.cn, hljiang@mail.shcnc.ac.cn The international journal of biochemistry & cell biology 2005 Only protein-protein interactions imex curation imex curation Virus - Publications including interactions involving viral proteins luo-2005-6 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid experimental particp experimental participant identification experimental particp Luo H., Wu D., Shen C., Chen K., Shen X., Jiang H. Coronavirus - Interactions investigated in the context of Coronavirus xshen@mail.shcnc.ac.cn, hljiang@mail.shcnc.ac.cn The international journal of biochemistry & cell biology 2005 Only protein-protein interactions imex curation imex curation Virus - Publications including interactions involving viral proteins ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid vme1_sars Membrane protein M E1 glycoprotein Matrix glycoprotein Membrane glycoprotein 5 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MADNGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ n-m-12 3724433 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P59596:p.Leu218_Leu219delinsAlaAla L218L219-A218A219 mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false 102-221 binding region 102-221 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 8 n-m-13 3724433 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 102-221 binding region 102-221 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 6 n-m-14 3724433 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 197-221 binding region 197-221 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig 6 n-m-15 3724433 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 341-422 binding region 341-422 binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 102-221 binding region 102-221 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false HisTag HisTag hypro hydroxylated proline HyPro n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 4 n-m-16 3724433 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HisTag HisTag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 283-422 binding region 283-422 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 102-221 binding region 102-221 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false HisTag HisTag hypro hydroxylated proline HyPro n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 4 n-m-10 3724434 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 291-422 binding region 291-422 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 102-221 binding region 102-221 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast physical association physical association false false false Fig 2 n-m-11 3724434 N/A 3724435 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed yeast Saccharomyces cerevisiae Baker's yeast N/A 3724436 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 102-221 binding region 102-221 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 197-221 binding region 197-221 binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast physical association physical association false false false Fig 2