IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ luo-2005-5 in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Luo H., Ye F., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins Cross-linking agent - glutaraldehyde xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Biochemistry 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus luo-2005-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Luo H., Ye F., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Biochemistry 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus luo-2005-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Luo H., Ye F., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Biochemistry 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus luo-2005-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Luo H., Ye F., Chen K., Shen X., Jiang H. Virus - Publications including interactions involving viral proteins xshen@mail.shcnc.ac.cn,hljiang@mail.shcnc.ac.cn Biochemistry 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid n-n-6 3709678 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figs. 4a, 6a The amount of multimers increased with the increase of the glutaraldehyde concentration. n-n-8 3709678 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4b The amount of multimers increased with the increase of the glutaraldehyde concentration. n-14 3709678 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4b The amount of dimers increased with the increase of the glutaraldehyde concentration n-n-13 3709678 N/A 3709682 inhibitor inhibitor neutral component neutral component purified purified SR rich peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6a n-16 3709678 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6a n-n-14 3709678 N/A 3709682 inhibitor inhibitor neutral component neutral component purified purified SR rich peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6a n-17 3709678 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 direct interaction direct interaction false false false Fig. 4b The amount of trimers increased with the increase of the glutaraldehyde concentration n-n-9 3709679 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 5b n-n-11 3709679 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6b n-n-12 3709679 N/A 3709682 inhibitor inhibitor neutral component neutral component purified purified SR rich peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N10 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6b n-n-15 3709679 N/A 3709682 inhibitor inhibitor neutral component neutral component purified purified SR rich peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6c n-18 3709679 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N11 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Figs. 5b, 6c n-n-7 3709679 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N12 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3709682 unspecified role unspecified role neutral component neutral component purified purified N12 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 3b n-n-4 3709680 N/A 3709682 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed delN5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3709682 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Nc region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1b n-n-5 3709681 N/A 3709682 unspecified role unspecified role prey prey purified purified SR rich peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3709682 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2a