HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html watashi-2005-1 Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Watashi K., Ishii N., Hijikata M., Inoue D., Murata T., Miyanari Y., Shimotohno K. Virus - Publications including interactions involving viral proteins Blasting the primers given the resulting UniProt entry corresponds to W5RLT6 (100% match), but as this is a Trembl entry the next highest match (95%) reviewed entry Q9WMX2 is used. kshimoto@virus.kyoto-u.ac.jp Mol. Cell (1097-2765) 2005 imex curation Only protein-protein interactions imex curation watashi-2005-5 Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Watashi K., Ishii N., Hijikata M., Inoue D., Murata T., Miyanari Y., Shimotohno K. Virus - Publications including interactions involving viral proteins Blasting the primers given the resulting UniProt entry corresponds to W5RLT6 (100% match), but as this is a Trembl entry the next highest match (95%) reviewed entry Q9WMX2 is used. kshimoto@virus.kyoto-u.ac.jp Mol. Cell (1097-2765) 2005 imex curation Only protein-protein interactions imex curation watashi-2005-2 Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Watashi K., Ishii N., Hijikata M., Inoue D., Murata T., Miyanari Y., Shimotohno K. Virus - Publications including interactions involving viral proteins Experiments in figures 3B, 3F and 6B were done using MH14 cells, a derivative of the Huh7 cell line, which carries stable HCV subgenomic replicons. Blasting the primers given the resulting UniProt entry corresponds to W5RLT6 (100% match), but as this is a Trembl entry the next highest match (95%) reviewed entry Q9WMX2 is used. kshimoto@virus.kyoto-u.ac.jp Mol. Cell (1097-2765) 2005 imex curation Only protein-protein interactions imex curation watashi-2005-3 Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Watashi K., Ishii N., Hijikata M., Inoue D., Murata T., Miyanari Y., Shimotohno K. Virus - Publications including interactions involving viral proteins The MH14 cells used in this experiment are a derivative of the Huh7 cell line, which carries stable HCV subgenomic replicons. Blasting the primers given the resulting UniProt entry corresponds to W5RLT6 (100% match), but as this is a Trembl entry the next highest match (95%) reviewed entry Q9WMX2 is used. kshimoto@virus.kyoto-u.ac.jp Mol. Cell (1097-2765) 2005 imex curation Only protein-protein interactions imex curation watashi-2005-4 Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Watashi K., Ishii N., Hijikata M., Inoue D., Murata T., Miyanari Y., Shimotohno K. Virus - Publications including interactions involving viral proteins The MH14 cells used in this experiment are a derivative of the Huh7 cell line, which carries stable HCV subgenomic replicons. Blasting the primers given the resulting UniProt entry corresponds to W5RLT6 (100% match), but as this is a Trembl entry the next highest match (95%) reviewed entry Q9WMX2 is used. kshimoto@virus.kyoto-u.ac.jp Mol. Cell (1097-2765) 2005 imex curation Only protein-protein interactions imex curation q9wmx2-pro_0000037552 RNA-directed RNA polymerase protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SMSYTWTGALITPCAAEETKLPINALSNSLLRHHNLVYATTSRSASLRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSVEEACKLTPPHSARSKFGYGAKDVRNLSSKAVNHIRSVWKDLLEDTETPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSTLPQAVMGSSYGFQYSPGQRVEFLVNAWKAKKCPMGFAYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIRSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKAAAACRAAKLQDCTMLVCGDDLVVICESAGTQEDEASLRAFTEAMTRYSAPPGDPPKPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIQRLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYLFNWAVRTKLKLTPIPAASQLDLSSWFVAGYSGGDIYHSLSRARPRWFMWCLLLLSVGVGIYLLPNR 2420 3010 ppib_human Peptidyl-prolyl cis-trans isomerase B PPIB CYPB Cyclophilin B S-cyclophilin CYP-S1 Rotamase B protein protein human Homo sapiens Human MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE cyclosporin_a Cyclosporin A small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ppib-q9wmx2_pro-1 1562639 n/a 1562644 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1562645 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A, 3E, 3G and 6A cyclosporin A ppib-q9wmx2_pro-2 1562639 n/a 1562646 inhibitor inhibitor unspecified role unspecified role tolin Tolypocladium inflatum n/a 1562645 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1562644 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 3E cyclosporin A q9wmx2_pro-ppib-3 1562640 n/a 1562645 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P23284:p.[Arg95Ala;Phe100Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1562644 unspecified role unspecified role bait bait Q9WMX2-PRO_0000037552:p.Pro540Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B q9wmx2_pro-ppib-1 1562640 n/a 1562645 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562644 unspecified role unspecified role bait bait physical association physical association false false false 3F Cyclosporin a (CHEBI:4031) q9wmx2_pro-ppib-2 1562641 n/a 1562645 unspecified role unspecified role prey prey n/a 1562644 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9WMX2-PRO_0000037552:p.Pro540Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3D, 6B p27958_pro-ppib-2 1562642 n/a 1562645 unspecified role unspecified role prey prey n/a 1562644 unspecified role unspecified role bait bait physical association physical association false false false 3C p27958_pro-ppib-1 1562643 n/a 1562645 unspecified role unspecified role neutral component neutral component n/a 1562644 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4A