IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ he-2004-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined He R., Dobie F., Ballantine M., Leeson A., Li Y., Bastien N., Cutts T., Andonov A., Cao J., Booth TF., Plummer FA., Tyler S., Baker L., Li X. Virus - Publications including interactions involving viral proteins Runtao_He@hc-sc.gc.ca Biochemical and biophysical research communications 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus he-2004-3 chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed gal4 vp16 complement gal4 vp16 complementation karyoplasmic interaction ion strategy KISS mammalian two hybrid gal4 vp16 complement predetermined predetermined participant predetermined He R., Dobie F., Ballantine M., Leeson A., Li Y., Bastien N., Cutts T., Andonov A., Cao J., Booth TF., Plummer FA., Tyler S., Baker L., Li X. Virus - Publications including interactions involving viral proteins Runtao_He@hc-sc.gc.ca Biochemical and biophysical research communications 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus he-2004-4 spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection density sedimentation cosedimentation through density gradient density sedimentation anti tag western anti tag western blot anti tag western He R., Dobie F., Ballantine M., Leeson A., Li Y., Bastien N., Cutts T., Andonov A., Cao J., Booth TF., Plummer FA., Tyler S., Baker L., Li X. Virus - Publications including interactions involving viral proteins Runtao_He@hc-sc.gc.ca Biochemical and biophysical research communications 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid n-n-10 3711130 N/A 3711133 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3711133 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 n-n-16 3711131 N/A 3711133 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3711133 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed SR-rich region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figs. 2, 4a-c n-19 3711132 N/A 3711133 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3711133 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig. 3