HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html derbigny-2000-2 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation derbigny-2000-4 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation derbigny-2000-3 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation derbigny-2000-5 mouse-lm mouse fibroblast L-M cells Mouse lm Murine fibroblast L-M cells anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation derbigny-2000-1 mouse-lm mouse fibroblast L-M cells Mouse lm Murine fibroblast L-M cells crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins dithiobis(succinimidylpropionate) used as cross linker DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation derbigny-2000-6 horse-tcc horse Horse Horse tcc transitional cell carcinoma cells confocal microscopy confocal microscopy immunostaining immunostaining Derbigny WA., Kim SK., Caughman GB., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins DOCALL@LSUMC.EDU DOCALL @LSUMC.EDU Journal of virology 2000 imex curation Only protein-protein interactions imex curation icp22_ehv1k Transcriptional regulator ICP22 homolog IR4 IR4 protein Immediate-early protein IE68 In vitro host-range factor ORF S1-2 protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MPHGQPCGACDGSCRMAQRGTPSTSPLIPSLTPSPPAGDPSPRSSQRIDAVRVPARLPGGSDHPEYGMPLSPRALRPYLARGPGAFCAPPWRPDVNRLAGDVNRLFRGISTSSIHVTEDSRTLRRALLDFYAMGYTHTRPTLECWQSLLQLLPEQSFPLRATLRALNSEDRYEQRFLEPPSDPPNTLFGEECDVSGDESPSEEEEEDEASGESSVSEFSPEEETASSEYDSFSDVGEDDSSCTGKWSSSESESDSESDAPTNNHHPTTRASAAKKRRKRQPPKGERPTKSARR icp4_ehv1k Major viral transcription factor IE 155 kDa immediate-early protein protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MASQRSDFAPDLYDFIESNDFGEDPLIRAASAAEEGFTQPAAPDLLYGSQNMFGVDDAPLSTPVVVIPPPSPAPEPRGGKAKRSPSAAGSGGPPTPAAAAQPASPAPSPAPGLAAMLKMVHSSVAPGNGRRATGSSSPGGGDAADPVALDSDTETCPGSPQPEFPSSASPGGGSPAPRVRSISISSSSSSSSSMDEDDQADGAGASSSSSSSSDDSDSDEGGEEETPRPRHSQNAAKTPSAAGSPGPSSGGDRPAAGAATPKSCRSGAASPGAPAPAPASAPAPSRPGGGLLPPGARILEYLEGVREANLAKTLERPEPPAGMASPPGRSPHRLPKDQRPKSALAGASKRKRANPRPRPQTQTQAPAEEAPQTAVWDLLDMNSSQATGAAAAAASAPAAASCAPGVYQREPLLTPSGDPWPGSDPPPMGRVRYGGTGDSRDGLWDDPEIVLAASRYAEAQAPVPVFVPEMGDSTKQYNALVRMVFESREAMSWLQNSKLSGQDQNLAQFCQKFIHAPRGHGSFITGSVANPLPHIGDAMAAGNALWALPHAAASVAMSRRYDRTQKSFILQSLRRAYADMAYPRDEAGRPDSLAAVAGCPAQAAAAAASQQQPEAPAPSVRVREAYTRVCAALGPRRKAAAAAAAPGTRAPRPSAFRLRELGDACVLACQAVFEALLRLRGGASAVPGLDPSEIPSPACPPEALCSNPAGLETAALSLYELRDLVERARLLGDSDPTHRLGSDELRLAVRAVLVVARTVAPLVRYNAEGARARASAWTVTQAVFSIPSLVGGMLGEAVSLLAPPTRSQQPSSSSPGGEPFSGSAAAEGSLQTLPPLWPTVPGKQSATVPSSHSQSPQHSQSGGGAGATTATCCRATQTNARSRGQQHQPQKARSPQAAASPAHLSQEAMPGSSSDDRAIHGRPRGKSGKRRSEPLEPAAQAGASASFSSSARGYDPSGPVDSPPAPKRRVATPGHQAPRALGPMPAEGPDRRGGFRRVPRGDCHTPRPSDAACAAYCPPELVAELIDNQLFPEAWRPALTFDPQALATIAARCSGPPARDGARLGELAASGPLRRRAAWMHQIPDPEDVKVVVLYSPLQDEDLLGGLPASRPGGSRREPLWSDLKGGLSALLAALGNRILTKRSHAWAGNWTGAPDVSALNAQGVLLLSTGDLAFTGCVEYLCLRLGSARRKLLVLDAVSTEDWPQDGPAISQYHIYMRAALTPRVACAVRWPGERHLSRAVLTSSTLFGPGLFARAEAAFARLYPDSAPLRLCRSSNVAYTVDTRAGERTRVPLAPREYRQRVLPDYDGCKDMRAQAEGLGFHDPDFEEGAAQSHRAANRWGLGAWLRPVYLACGRRGAGAVEPSELLIPELLSEFCRVALLEPDAEAEPLVLPITEAPRRRAPRVDWEPGFGSRSTSVLHMGATELCLPEPDDELEIDGAGDVELVVEHPGPSPGVAQALRRAPIKIEVVSDDEDGGDWCNPYLS pp11_yeast Serine/threonine-protein phosphatase PP1-1 SIT4 PPH1 YDL047W D2693 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSRGPDEWLETIKKCQALTENEMKQLCEMVKELLMEESNIQPVQTPVTVCGDIHGQFHDLLELFRTAGGFPDDINYIFLGDYVDRGYYSLETFTLLMCLKVKYPAKITLVRGNHESRQITQVYGFYEECLNKYGSTTVWKYCCQVFDFLTLAAIIDGKILCVHGGLSPEIRMLDQIRVLSRAQEVPHEGGFSDLLWSDPDNVEAWQVSPRGAGWLFGSKVAREFNHVNGLNLIARAHQLVMEGFKYHFPEKDVVTVWSAPNYCYRCGNVASVMKVDEDLEPTFKIFSAVPDDYIRESTANHNNQRAGYFL AF52BC65E4E715EC gac1_yeast Serine/threonine-protein phosphatase 1 regulatory subunit GAC1 YOR178C GAC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVIQTATTLSPAKARPSFPHNDLIKSMSDSLISRPTHPPIRKLKSSLKISHPEPISRSKSEIFCTSPEKNVRFAIELTTVKRFDKNAEPSSISNENSPTLSPVDSNTAADDVQLFNNEDCWFNDSSLVTNLLKNEKKFRYMNSLNNMFKLDLYDSEDEDDIDEHINSQAEYGYTYNSLSTRGKTSENKSATSSLATQATNICDWKLHCTDLVPFKIAPPLFTKTLSASDLQGQLTKYLNGQNVKLHSLTQLGDDSSKITGLVYVKNLSFEKYLEIKFTFNSWRDIHYVTANFNRTINSNVDEFKFTIDLNSLKYILLIKRIITMEKNTSSCPLNIELCCRYDVNNETYYDNNNGKNYHLFMTTFKKGGETKEKIPVVVEPASQTDAAMSPKEMKARFVSSNPTLSRFLPQSRKFSEDTDYYNTSPLKHLYHNDTTSWVKPKRLNVVLDKLENATPPPPSSALANDTARTGKITKDKNNVLAPPTASNSIDLPILGSQHQSLYSGSSSYSSSSSSISSSLSFASSNNSSTNSSSASCSFPLTELDNFDYANLYEPNDTFTTANLFNHSLNSLMPEISTPSFFGGFRNENTINNNDSKNLVTSLEDSYEDKQSVITDTTMDENNKTSTINNSTDTLIKPSKENGTVKENKSSANSTSAPSSSQNRASTILNDHSNGKSDLKYVNYQSLLDSHCFYNHPSSPNLQSTSFSSAAPFSGISQASDIFDYENEDSDSNQIAGEIDNNSFPPHFYLDEDDKSACLSDDALIDHHRNTNPFINTFSSSPPILSQEVDRWRL 1880E966A6480D80 icp22-icp22-2 1938376 N/A 1938382 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 1938382 unspecified role unspecified role bait bait physical association physical association false false false table 1 & 2 icp22-ie-1 1938376 N/A 1938383 unspecified role unspecified role prey prey N/A 1938382 unspecified role unspecified role bait bait physical association physical association false false false table 1 & 2 ie-icp22-2 1938376 N/A 1938383 unspecified role unspecified role bait bait N/A 1938382 unspecified role unspecified role prey prey physical association physical association false false false table 1 ie-ir4-1 1938376 N/A 1938383 unspecified role unspecified role prey prey N/A 1938383 unspecified role unspecified role bait bait physical association physical association false false false table 1 gac1-sit4-1 1938376 N/A 1938384 unspecified role unspecified role prey prey N/A 1938385 unspecified role unspecified role bait bait physical association physical association false false false table 1 sit4-gac1-1 1938376 N/A 1938384 unspecified role unspecified role bait bait N/A 1938385 unspecified role unspecified role prey prey physical association physical association false false false table 1 ie-ir4-3 1938377 N/A 1938383 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ir2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 1938382 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1938411 1938408 physical association physical association false false false 3A & 8 ir4-ir4-1 1938377 N/A 1938382 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1938382 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A & 7 ir4-ie-1 1938377 N/A 1938382 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1938382 competitor competitor competitor unspecified role unspecified role N/A 1938383 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 8B icp22-ie-2 1938378 N/A 1938383 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1938382 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2 ie-ir4-2 1938378 N/A 1938383 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1938382 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2 ir4-ie-2 1938379 N/A 1938383 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1938382 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4 ie-ir4-4 1938379 N/A 1938383 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1938382 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4 icp22-icp22-1 1938380 N/A 1938382 unspecified role unspecified role neutral component neutral component N/A 1938382 unspecified role unspecified role neutral component neutral component physical association physical association false false false 1 ir4-ie-3 1938381 N/A 1938383 unspecified role unspecified role neutral component neutral component N/A 1938382 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5