HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html jeyaseelan-2000-1 in vitro In vitro solid phase assay solid phase assay western blot western blot Immuno blot Immuno blot Jeyaseelan S., Hsuan SL., Kannan MS., Walcheck B., Wang JF., Kehrli ME., Lally ET., Sieck GC., Maheswaran SK. Virus - Publications including interactions involving viral proteins Polystyrene beads coated with Lkt or BSA were incubated with lysates from the different cells. Bound proteins eluted from the beads were separated by SDS-PAGE under nonreducing conditions, transferred to a polyvinylidene difluoride membrane, and probed with MAbs against CD11a, and CD18. mahes001@maroon.tc.umn.edu Infection and immunity 2000 imex curation Only protein-protein interactions imex curation itb2_bovin Integrin beta-2 Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta Complement receptor C3 subunit beta ITGB2 CD18 protein protein bovin Bos taurus (Bovine) Bovine MLRQRPQLLLLAGLLALQSVLSQECTNYKVSTCRDCIESGPGCAWCQKLNFTGQGEPDSIRCDTRAELLSKGCPADDIMEPKSLAETRDSQAGSRKQLSPQEVTLYLRPGQAVAFNVTFRRAKGYPIDLYYLMDLSYSMVDDLVNVKKLGGDLLRALNGITESGRIGFGSFVDKTVLPFVNTHPEKLRNPCPNKEKECQPPFAFRHVLKLTDNSKQFETEVGKQLISGNLDAPEGGLDAMMQVAACPEEIGWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKSSNEFDYPSVGQLAHKLAESNIQPIFAVTKKMVKTYEKLTEIIPKSAVGELSEDSRNVVELIKNAYNKLSSRVFLDHSTLPDTLKVTYDSFCSNGKSQVDQPRGDCDGVQINVPITFQVKVTATECIQQQSFTIRALGFTDTVTVRVLPQCECQCRDASRDGSICGGRGSMECGVCRCDAGYIGKNCECQTQGRSSQELEGSCRKDNSSIICSGLGDCICGQCVCHTSDVPNKKIYGQFCECDNVNCERYDGQVCGGEKRGLCFCGTCRCDEQYEGSACQCLKSTQGCLNLDGVECSGRGRCRCNVCQCDPGYQPPLCSECPGCPVPCAGFAPCTECLKFDKGPFAKNCSAACGQTKLLSSPVPGRKCKERDSEGCWMTYTLVQRDGRDRYDVHVDDMLECVKGPNIAAIVGGTVGGVVLVGILLLVIWKALTHLSDLREYHRFEKEKLKSQWNNDNPLFKSATTTVMNPKFAES lka1b_manha Leukotoxin lktA protein protein manha manha Pasteurella haemolytica MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKIILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTERGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDEALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGDKLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITKAVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERFKKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSGITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMKFLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKADKLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRVDSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGTTEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALVGNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDTIDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIITDSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKATKDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLDKLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA ital_bovin Integrin alpha-L ITGAL CD11 antigen-like family member A Leukocyte adhesion glycoprotein LFA-1 alpha chain Leukocyte function-associated molecule 1 alpha chain protein protein bovin Bos taurus (Bovine) Bovine MNSCIIVLRLLLSGPFVFAPAWSYNLDVRHVQNFSFPLAGRHFGYRVLQVGNGVVVGAPSEGNSMGNLYQCQPETGDCLPVTLSSNYTSKYLGMTLATDPTSDNLLACDPGLSRTCDQNIYLSGLCYLIHENLRGPVLQGHPGYQECIKGNVDLVFLFDGSMSLQQDEFEKIVDFMKDVMKKLSNSSYQFAAVQFSTYFRTEFTFLDYIRQKDPDALLAGVKHMRLLTNTFGAINYVAKEVFRPDLGARPDATKVLIIITDGEATDEHNIDAAKDIIRYIIGIGKNFKTKESQEALHQFASKPVEEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSEGHGVVGAVGAKDWAGGFLDLKADLKSSTFVGNEPLTVESRAGYLGYTVTWLPSRGTMSLLATGAPRYQHVGRVLLFQQPKRGGPWSQIQEIDGIQIGSYFGGELCGVDVDRDGETELLLIAAPLYYGEQRGGRVFIYQKIQLEFQMVSELQGETGYPLGRFGAAIAALTDINGDELTDVAVGAPLEEQGAVYIFNGQQGGLSPRPSQRIEGTQMFSGIQWFGRSIHGVKDLGGDGLADVAVGAEGQVIVLSSRPVVDIITSVSFSPAEIPVHEVECSYSTSNQKKEGVNLTVCFQVKSLISTFQGHLVANLTYTLQLDGHRTRSRGLFPGGKHKLIGNTAVTPVKSCFVFWFHFPICIQDLISPINVSLSYSLWEEEGTPRDPRALDRDIPPILKPSPHLETKEIPFEKNCGEDKNCEADLKLAFSDMRSKILRLTPSASLSVRLTLRNTAEDAYWVQVTLSFPQGLSFRKVEILKPHSHVPVGCEELPEEAVVHSRALSCNVSSPIFGEDSMVDIQVMFNTLQKGSWGDFIELQANVSCNNEDSSLLEDNSATTSIPVMYPINVLTKDQENSTLYISFTPKSPKIHHVKHIYQVRIQPSNYDNMPPLEALVRVPRVHSEGLITHKWSIQMEPPVNCSPRNLESPSDEAESCSFGTEFRCPIDFRQEILVQVNGMVELRGTIKASSMLSLCSSLAISFNSSKHFHLYGRNASMAQVVMKVDLVYEKEMLYLYVLSGIGGLLLLFLIFIALYKVGFFKRNLKEKMEANVDASSEIPGEDAGQPELEKECKDPGCLEPLQKTDEDGSGGD lkt-cd18-2 1923185 N/A 1923186 unspecified role unspecified role prey prey bovin-neutrophil bovin neutrophil Bovine neutrophil Neutrophil [Neutrophilic granulocyte] N/A 1923187 unspecified role unspecified role bait bait physical association physical association false false false 2A and table 2 neutrophils from calves with bovine leukocyte adhesion deficiency (BLAD) syndrome are also used for interaction assay lkta-cd11a-1 1923185 N/A 1923188 unspecified role unspecified role prey prey bovin-neutrophil bovin neutrophil Bovine neutrophil Neutrophil [Neutrophilic granulocyte] N/A 1923187 unspecified role unspecified role bait bait physical association physical association false false false 2B and table 2 neutrophils from calves with bovine leukocyte adhesion deficiency (BLAD) syndrome are also used for interaction assay lkta-cd18-1 1923185 N/A 1923186 unspecified role unspecified role prey prey bovin-alveolar macrophages bovin alveolar macrophages Bovine alveolar_macrophage Alveolar macrophage N/A 1923187 unspecified role unspecified role bait bait physical association physical association false false false 2A and table 2 lkta-cd11a-2 1923185 N/A 1923188 unspecified role unspecified role prey prey bovin-alveolar macrophages bovin alveolar macrophages Bovine alveolar_macrophage Alveolar macrophage N/A 1923187 unspecified role unspecified role bait bait physical association physical association false false false 2B and table 2