IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2005d-1 Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org J. Leukoc. Biol. (0741-5400) 2005 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease zhao-2005d-2 Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. human-monocyte Human mononuclear cells monocyte Monocyte, mononuclear cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org J. Leukoc. Biol. (0741-5400) 2005 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease kpcd_human Protein kinase C delta type nPKC-delta PRKCD Tyrosine-protein kinase PRKCD PKCD protein protein human Homo sapiens Human MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED ncf2_human Neutrophil cytosol factor 2 NOXA2 P67PHOX Neutrophil NADPH oxidase factor 2 NADPH oxidase activator 2 67 kDa neutrophil oxidase factor p67-phox NCF2 protein protein human Homo sapiens Human MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV pkctide Protein kinase C (PKC) consensus substrate peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ERMRPRKRQGSVRRRV ncf2-prkcd 1766292 n/a 1766294 enzyme enzyme neutral component neutral component spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. n/a 1766295 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false Fig. 4c Q05655 is required for phosphorylation of P19878 P19878 phosphorylation CHEBI:37671 increases binding of Q05655 to P19878 Q05655 increases activity of P19878 CHEBI:37671 indirectly increases activity of P19878 prkcd-1 1766292 n/a 1766294 putative self putative self putative self putative self spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation false true false Fig. 4c Q05655 is required for phosphorylation of Q05655 Q05655 phosphorylation pkctide-prkcd 1766292 n/a 1766296 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1766294 enzyme enzyme neutral component neutral component spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation false false false Fig. 4c EBI-10040557 ser-11 O-phospho-L-serine prkcd-ncf2 1766293 n/a 1766294 unspecified role unspecified role bait bait n/a 1766295 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3a zymosan (opsonised) (ZOP) (CHEBI:37671) No p67phox co-immunoprecipitated with anti-PKC-delta antibodies from cells not treated with ZOP. ncf2-prkcd-2 1766293 n/a 1766294 unspecified role unspecified role prey prey n/a 1766295 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3b zymosan (opsonised) (ZOP) (CHEBI:37671) No PKC-delta co-immunoprecipitated with anti-p67phox antibodies from cells not treated with ZOP.