IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nishimune-1998-3 NSF binding to GluR2 regulates synaptic transmission. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nishimune A., Isaac JT., Molnar E., Noel J., Nash SR., Tagaya M., Collingridge GL., Nakanishi S., Henley JM. Synapse - Interactions of proteins with an established role in the presynapse. 3 No email provided. Dr Henley :Department of Pharmacological Sciences, University of Newcastle upon Tyne, the Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. rat brain cDNA library was used Neuron (0896-6273) 1998 Only protein-protein interactions imex curation nishimune-1998-2 NSF binding to GluR2 regulates synaptic transmission. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nishimune A., Isaac JT., Molnar E., Noel J., Nash SR., Tagaya M., Collingridge GL., Nakanishi S., Henley JM. Synapse - Interactions of proteins with an established role in the presynapse. The specificity of gria2-nsf binding was tested by transforming yeast containing NSF with the c-terminal domains of subunits GluR1, GluR2, GluR3, GluR4, GluR6 and NMDAR1. Activation of the LacZ reporter was determined in liquid beta-galactosidase assays. 1a and 1d No email provided. Dr Henley :Department of Pharmacological Sciences, University of Newcastle upon Tyne, the Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. rat brain cDNA library was used Neuron (0896-6273) 1998 Only protein-protein interactions imex curation nishimune-1998-4 NSF binding to GluR2 regulates synaptic transmission. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nishimune A., Isaac JT., Molnar E., Noel J., Nash SR., Tagaya M., Collingridge GL., Nakanishi S., Henley JM. Synapse - Interactions of proteins with an established role in the presynapse. 2b and c No email provided. Dr Henley :Department of Pharmacological Sciences, University of Newcastle upon Tyne, the Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. rat brain cDNA library was used Neuron (0896-6273) 1998 Only protein-protein interactions imex curation nishimune-1998-1 NSF binding to GluR2 regulates synaptic transmission. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Nishimune A., Isaac JT., Molnar E., Noel J., Nash SR., Tagaya M., Collingridge GL., Nakanishi S., Henley JM. Synapse - Interactions of proteins with an established role in the presynapse. No email provided. Dr Henley :Department of Pharmacological Sciences, University of Newcastle upon Tyne, the Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. rat brain cDNA library was used Neuron (0896-6273) 1998 Only protein-protein interactions imex curation nsf_crigr Vesicle-fusing ATPase NSF N-ethylmaleimide-sensitive fusion protein Vesicular-fusion protein NSF protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAGRSMQAARCPTDELSLSNCAVVSEKDYQSGQHVIVRTSPNHKYIFTLRTHPSVVPGSVAFSLPQRKWAGLSIGQEIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD gria2_rat Glutamate receptor 2 Gria2 Glur2 GluR-B GluR-K2 Glutamate receptor ionotropic, AMPA 2 AMPA-selective glutamate receptor 2 protein protein rat Rattus norvegicus (Rat) Rat MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI gria3_rat Glutamate receptor 3 Gria3 Glur3 GluR-K3 Glutamate receptor ionotropic, AMPA 3 AMPA-selective glutamate receptor 3 GluR-C protein protein rat Rattus norvegicus (Rat) Rat MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLSYYKWEKFVYLYDTERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMVQQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSSSSENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI nsf_rat Vesicle-fusing ATPase Nsf Erg1 N-ethylmaleimide-sensitive fusion protein Vesicular-fusion protein NSF protein protein rat Rattus norvegicus (Rat) Rat MAGRTMQAARCPTDELSLSNCAVVNEKDYQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNHQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD gria2-nsf-3 biochemical verification of the interaction between GluR2 and NSF, by affinity chromatography. 370494 N/A 370498 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 370499 unspecified role unspecified role bait bait c-terminal region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false The amount of NSF retained by GluR2 sepharose decreased markedly with sequential washes, suggesting that the interaction is readily reversible under the conditions used. Synthetic peoptides GluR2 (KRMKVAKNPQ) and GluR2m (KRMKVAKNAQ)prevented binding of NSF to GRIA2. gria3-nsf-2 biochemical verification of the interaction between GluR2 and NSF, by affinity chromatography. 370494 N/A 370498 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 370500 unspecified role unspecified role bait bait c-terminal region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. low - observed only under relatively mild wash conditiions. low - observed only under relatively mild wash conditiions. gria2-nsf-4 Biochemical verification of the interaction between GluR2 and NSF, by affinity chromatography. 370494 N/A 370499 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 370501 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association false false false Under these experimental conditions, the proportion of NSF retained by GluR2 is low, suggesting that other protein(s) may be involved in the in vivo interaction. gria2-nsf-2 Filter beta-galactosidase assay for detection of interaction between ct-GluR2 and NSF 370495 N/A 370499 unspecified role unspecified role unspecified role unspecified role c-terminal region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 370501 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false all domains of the NSF protein were found to be necessary for binding to NSF. gria3-nsf-1 weak interaction between ct-GluR3 and NSF detected by beta galactosidase assay in yeast 370495 N/A 370500 unspecified role unspecified role unspecified role unspecified role c-terminal region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 370501 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false not observed using liquid beta gal assay gria2-nsf-5 Analysis of NSF interaction with deletion mutants of ct-GluR2 370496 N/A 370501 unspecified role unspecified role prey prey N/A 370499 unspecified role unspecified role bait bait c-terminal region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false gria2-nsf-6 Analysis of NSF interaction with deletion mutatns of ct-GluR2 370496 N/A 370501 unspecified role unspecified role prey prey N/A 370499 unspecified role unspecified role bait bait c-terminal region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false gria2-nsf-1 Detection of the Interaction between ct-GluR2 and NSF, by yeast two hybrid. 370497 N/A 370499 unspecified role unspecified role bait bait c-terminus region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 370501 unspecified role unspecified role prey prey physical association physical association false false false