IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mor-2008-2 Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 Synapse - Interactions of proteins with an established role in the presynapse. mor-2008-3 Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 Synapse - Interactions of proteins with an established role in the presynapse. mor-2008-1 Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 Synapse - Interactions of proteins with an established role in the presynapse. acetylcholine Acetylcholine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) aces_human Acetylcholinesterase ACHE protein protein human Homo sapiens Human MRPPQCLLHTPSLASPLLLLLLWLLGGGVGAEGREDAELLVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL enoa_human Alpha-enolase ENO1 ENO1L1 MBPB1 MPB1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 Phosphopyruvate hydratase C-myc promoter-binding protein MBP-1 MPB-1 Plasminogen-binding protein protein protein human Homo sapiens Human MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK 2-phospho-d-glyceric acid 2-phospho-D-glyceric acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) human_arp26_peptide ARP26 peptide based on the C-terminal sequence of the AChE-R isoform of human acetylcholinesterase peptide peptide polypeptide oligopeptide oligopeptide polypeptide human Homo sapiens Human GMQGPAGSGWEEGSGSPPGVTPLFSP An amino acid sequence which is not represented in UniProt rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR rack1_mouse Receptor of activated protein C kinase 1 p205 Receptor of activated protein kinase C 1 12-3 Gnb2-rs1 Gnb2l1 Guanine nucleotide-binding protein subunit beta-2-like 1 Rack1 Receptor for activated C kinase protein protein mouse Mus musculus Mouse MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR enoa_mouse Alpha-enolase Eno1 Eno-1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 protein protein mouse Mus musculus Mouse MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLAK q9jjp2-1 Tumor protein p73 Tp73 P73 Trp73 p53-like transcription factor p53-related protein Alpha protein protein mouse Mus musculus Mouse MAQTSSSSSSTFEHLWSSLEPDSTYFDLPQPSQGTSEASGSEESNMDVFHLQGMAQFNLLSSAMDQMGSRAAPASPYTPEHAASAPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLAQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILVIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESTTKNGAASKRAFKQSPPAIPALGTNVKKRRHGDEDMFYMHVRGRENFEILMKVKESLELMELVPQPLVDSYRQQQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAAGPNLGPMGSGMLNSHGHSMPANGEMNGGHSSQTMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIECFTSQGLQSIYHLQNLTIEDLGALKVPDQYRMTIWRGLQDLKQSHDCGQQLLRSSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGAGAVTGPDEWADFGFDLPDCKSRKQPIKEEFTETESH q9jjp2-2 Tumor protein p73 Tp73 P73 Trp73 p53-like transcription factor p53-related protein dN-Alpha protein protein mouse Mus musculus Mouse MLYVGDPMRHLATAQFNLLSSAMDQMGSRAAPASPYTPEHAASAPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLAQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILVIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESTTKNGAASKRAFKQSPPAIPALGTNVKKRRHGDEDMFYMHVRGRENFEILMKVKESLELMELVPQPLVDSYRQQQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAAGPNLGPMGSGMLNSHGHSMPANGEMNGGHSSQTMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIECFTSQGLQSIYHLQNLTIEDLGALKVPDQYRMTIWRGLQDLKQSHDCGQQLLRSSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGAGAVTGPDEWADFGFDLPDCKSRKQPIKEEFTETESH Produced by alternative promoter usage. ache-acetylcholine ACHE (AChE-R) displays acetylcholinesterase activity in vitro 734535 N/A 734538 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 734539 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana N/A 734540 stimulator stimulator unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 1 C Incubation with enolase-alpha increased ACHE's acetylcholinesterase activity. Km = 4.6 E-5 M; Km (in presence of enolase-alpha = 5.5 E-5. Vmax = 12.6 arbitrary units/min; Vmax (in presence of enolase-alpha) = 15 arbitrary units/min. eno1-2_phospho_d Enolase-alpha enolase activity is enhanced by a peptide based on acetylcholinesterase 734535 N/A 734539 stimulator stimulator neutral component neutral component N/A 734540 enzyme enzyme unspecified role unspecified role N/A 734541 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 734542 stimulator stimulator neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 4 B The enolase-alpha activity of enolase-alpha was stimulated by the peptide ARP26 (GMQGPAGSGWEEGSGSPPGVTPLFSP), which is at the C-terminus of the form of ACHE referred to by the authors as AChE-R. The enolase-alpha activity of enolase-alpha was not stimulated by the peptide ASP23 (WSSYMVHWKQFDHYSKQDRCSDL), which is at the C-terminus of the form of ACHE referred to by the authors as AChE-S. ache-acetylcholine-1 ACHE (AChE-R) displays acetylcholinesterase activity in vitro 734535 N/A 734538 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 734539 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana N/A 734543 stimulator stimulator unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 1 C Incubation with GNB2L1 increased ACHE's acetylcholinesterase activity. Km = 4.6 E-5 M; Km (in presence of GNB2L1) = 4.8 E-5 M. Vmax = 12.6 arbitrary units/min; Vmax (in presence of GNB2L1) = 15 arbitrary units/min. ache-acetylcholine-2 ACHE (AChE-R) displays acetylcholinesterase activity in vitro 734535 N/A 734538 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 734539 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 1 C Km = 4.6 E-5 M. Vmax = 12.6 arbitrary units/min. ache-gnb2l1-2 ACHE (AChE-R) coimmunoprecipitates Gnb2l1 (RACK1) in mouse testicular homogenates 734536 N/A 734539 unspecified role unspecified role bait bait N/A 734544 unspecified role unspecified role prey prey physical association physical association false false false 1 D This interaction occurred in testicular homogenates from TgR mice, which overexpress human ACHE (hAChE-R). P22303 overexpressed eno1-ache Enolase-alpha coimmunoprecipitates ACHE (AChE-R) in mouse testicular homogenates 734536 N/A 734545 unspecified role unspecified role bait bait N/A 734539 unspecified role unspecified role prey prey physical association physical association false false false 1 D P22303 overexpressed This interaction occurred in testicular homogenates from TgR mice, which overexpress human ACHE (hAChE-R). gnb2l1-trp53 Gnb2l1 (RACK1) coimmunoprecipitates Trp73 (p73) and DeltaNp73 in mouse testicular homogenates 734536 n/a 734546 unspecified role unspecified role prey prey n/a 734547 unspecified role unspecified role prey prey N/A 734544 unspecified role unspecified role bait bait association association false false false 3 D ache-gnb2l1 ACHE (AChE-R) interacts with GNB2L1 (RACK1) in a yeast two-hybrid assay 734537 N/A 734543 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 734539 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 A, B ache-eno1-1 ACHE (AChE-R) interacts with enolase-alpha in a yeast two-hybrid assay 734537 N/A 734540 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 734539 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 A, B